Comparative properties of untreated andN-nitrosobenzylmethyl-amine-transformed rat esophageal epithelial cell lines

Abstract

A culture system utilizing rat esophageal epithelial cells has been developed. Four normal and eight N-nitrosobenzylmethylamine-treated lines were compared with respect to chromosome number, anchorage-independent growth in agarose, and tumorigenic potential in syngeneic rats. All cell lines were aneuploid with nine in the near-tetraploid range and three in the near-diploid range. No relation between tumorigenic potential and chromosome number or structure was apparent. Similarly, anchorage-independent growth in agarose did not correlate with tumorigenic potential. Three of the 12 immortalized lines (two carcinogen-treated and 1 untreated) induced well-differentiated squamous cell carcinomas in syngeneic rats. These tumors had weak metastatic potentials suggesting that tumorigenic potential and metastatic ability are separately controlled. These cell lines will be useful for the investigation of factors involved in the conversion of immortalized rat esophageal epithelial cell lines to lines of high metastatic potential.

Characterization of a monoclonal antibody reactive with a glycolipid antigen expressed by tumorigenic and certain immortalized, non-tumorigenic rat esophageal epithelial cell lines

“…Tumorigenic rat esophageal epithelial cell lines RE-35,B2, RE-35,B2T, RE-35,C 1 T, and non-tumorigenic, immortalized rat esophageal epithelial cell lines RE79, RE149 and RE282 were established and characterized by Stoner et al [25,26]. For simplicity, these cell lines are referred to as B2, B2T, C 1T, RE79, RE149 and RE282 respectively.…”

“…For simplicity, these cell lines are referred to as B2, B2T, C 1T, RE79, RE149 and RE282 respectively. The immortalized epithelial cell lines (RE79, RE149, and RE282), although non-tumorigenic, are aneuploid and exhibit other abnormalities [26]. In addition, four tumorigenic rat tracheal epithelial cell lines, BP2, BP3 [11], RTE2-2, RTE2-12 [15], one tumorigenic rat lung cell line, MCA7 [11], one tumorigenic rat urinary bladder epithelial cell line, AY-27 [4], and several non-tumorigenic, immortalized rat epithelial cell lines were included in this study.…”

“…Heterologous cell lines, used as controls, included two human esophageal carcinoma cell lines, TE-1 and TE-2 [18], one simian virus 40 T antigen immortalized human esophageal cell line, HET-1A [27], a human epidermoid carcinoma cell line, A431 [6], a tumorigenic mouse forestomach epithelial cell line, DEN3 [12] and a tumorigenic mouse lung epithelial cell line, fine 1 [34]. All rat cell lines were maintained in PFMR-4 medium (Biofluids) as described previously [25,26]. All heterologous cell lines, except HET-1A, were propagated in Dulbecco's modified Eagle medium (DMEM) or in McCoy's medium (Gibco) supplemented with 10% fetal bovine serum.…”

A Monoclonal Antibody Produced Against a Rat Esophageal Carcinoma Cell Line Reacts with an Integrin-Like Molecule Expressed by Rat Epithelial Cells

“…For simplicity, these cell lines are referred to as B-2, B-2T, C-1T, RE-79, RE-149 and RE-282, respectively. The origin and biological properties of these lines have been described (4,6). Briefly, cell line, B-2, was developed from an epithelial cell outgrowth of an expiant culture of rat esophagus that had been treated jn vitro with N-nitroscbenzylmethylamine (4,6).…”

“…To address this problem, we prepared a monoclonal antibody (MAb-5A) to a tumorigenic rat esophageal epithelial cell line, designated B-2T. The biological properties of this cell line in vivo and jn vitro have been reported (4). B-2T cells, upon injection into syngeneic newborn rats, produce well differentiated squamous cell carcinomas (SCO) histologically similar to SCO of the human esophagus (5).…”