Comparative proteome analyses of rhizomania resistant transgenic sugar beets based on RNA silencing mechanism

Hejri, Sara; Salimi, Azam; Malboobi, Mohammad Ali; Fatehi, Foad

doi:10.1080/21645698.2021.1954467

Cited by 8 publications

(5 citation statements)

References 60 publications

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“…This study presented data for investigating the substantial equivalence of the third generations of two homozygous GM sugar beets in comparison with their parental plant grown in the same conditions by measuring the levels of free amino acids and some key metabolites. Having confirmed the presence of transgene in the chosen events ( Hejri et al, 2021 ), half of the plants at six leaf stages were challenged with the virus for two months. The viral propagation inhibition by RNA-silencing mechanism against P21 of BNYVV in the transgenic events was reaffirmed by comparative RT-PCR.…”

Section: Resultsmentioning

confidence: 99%

Investigation of Possible Changes Induced by RNA Silencing in Some Leaf Metabolites of Transgenic Sugar Beet Events

Hejri

Salimi

Malboobi

et al. 2022

Food Chemistry: Molecular Sciences

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Section: Resultsmentioning

confidence: 99%

Investigation of Possible Changes Induced by RNA Silencing in Some Leaf Metabolites of Transgenic Sugar Beet Events

Hejri

Salimi

Malboobi

et al. 2022

Food Chemistry: Molecular Sciences

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“…However, little was known about the omics studies of hybrid progeny for gene drift assessment. Combined transcriptome and metabolome analysis of herbicide-resistant maize found that the influence of the environment was often more significant than that of exogenous gene insertion . PCA indicated that the protein composition of transgenic soybean was similar to that of its non-transgenic parent line, which is consistent with the previous study on the unintended effect of herbicide-resistant soybean …”

Section: Discussionmentioning

confidence: 99%

“…Combined transcriptome and metabolome analysis of herbicide-resistant maize found that the influence of the environment was often more significant than that of exogenous gene insertion. 48 PCA indicated that the protein composition of transgenic soybean was similar to that of its non-transgenic parent line, which is consistent with the previous study on the unintended effect of herbicide-resistant soybean. 49 Genomic findings on wild soybeans have found that some alleles of the wild soybean genome have been lost during the evolution of cultivated soybean.…”

Section: ■ Discussionmentioning

confidence: 99%

In Situ Proteomic Analysis of Herbicide-Resistant Soybean and Hybrid Seeds via Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging

Yin

Ren

Zhang

et al. 2023

J. Agric. Food Chem.

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Transgenic soybean is the commercial crop with the largest cultivation area worldwide. During transgenic soybean cultivation, exogenous genes may be transferred to wild relatives through gene flow, posing unpredictable ecological risks. Accordingly, an environmental risk assessment should focus on fitness changes and underlying mechanisms in hybrids between transgenic and wild soybeans (Glycine soja). Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) was used for in situ detection and imaging of protein changes in the seeds of transgenic herbicide-resistant soybean harboring epsps and pat genes, non-transgenic soybean, wild soybean, and their F 2 hybrid. Protein data clearly distinguished wild soybeans, while the F 2 seeds had protein characteristics of both parents and were distinguished from wild soybean seeds. Using UPLC-Q-TOF-MS, 22 differentially expressed proteins (DEPs) were identified, including 13 specific to wild soybean. Sucrose synthase and stress responserelated DEPs were differentially expressed in parental and offspring. Differences in these may underpin the greater adaptability of the latter. MSI revealed DEP distribution in transgenic, wild, and F 2 seeds. Identifying DEPs related to fitness may elucidate mechanisms underlying fitness differences among the studied varieties. Our study shows that MALDI-MSI has the potential to become a visual method for transgenic soybean analysis.

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“…Aluminum-activated malate transporter, which belongs to the anion channels, is located in the membranes of different tissues and has a various and fundamental range of physiological functions such as aluminum resistance, signaling, anion homoeostasis, osmotic adjustment, stomata regulation, and abiotic stress tolerance via transporting malate or inorganic anions ( Palmer et al., 2016 ; Ramesh et al., 2018 ; Hejri et al., 2021 ). Maintaining sufficient amounts of water is essential for plant growth and development ( Pasaribu et al., 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Proteomics approach to investigating osmotic stress effects on pistachio

et al. 2023

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Osmotic stress can occur due to some stresses such as salinity and drought, threatening plant survival. To investigate the mechanism governing the pistachio response to this stress, the biochemical alterations and protein profile of PEG-treated plants was monitored. Also, we selected two differentially abundant proteins to validate via Real-Time PCR. Biochemical results displayed that in treated plants, proline and phenolic content was elevated, photosynthetic pigments except carotenoid decreased and MDA concentration were not altered. Our findings identified a number of proteins using 2DE-MS, involved in mitigating osmotic stress in pistachio. A total of 180 protein spots were identified, of which 25 spots were altered in response to osmotic stress. Four spots that had photosynthetic activities were down-regulated, and the remaining spots were up-regulated. The biological functional analysis of protein spots exhibited that most of them are associated with the photosynthesis and metabolism (36%) followed by stress response (24%). Results of Real-Time PCR indicated that two of the representative genes illustrated a positive correlation among transcript level and protein expression and had a similar trend in regulation of gene and protein. Osmotic stress set changes in the proteins associated with photosynthesis and stress tolerance, proteins associated with the cell wall, changes in the expression of proteins involved in DNA and RNA processing occur. Findings of this research will introduce possible proteins and pathways that contribute to osmotic stress and can be considered for improving osmotic tolerance in pistachio.

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Comparative proteome analyses of rhizomania resistant transgenic sugar beets based on RNA silencing mechanism

Abstract: Fatehi (2021): Comparative proteome analyses of rhizomania resistant transgenic sugar beets based on RNA silencing mechanism, GM Crops & Food,

Cited by 8 publications

References 60 publications

Investigation of Possible Changes Induced by RNA Silencing in Some Leaf Metabolites of Transgenic Sugar Beet Events

Investigation of Possible Changes Induced by RNA Silencing in Some Leaf Metabolites of Transgenic Sugar Beet Events

In Situ Proteomic Analysis of Herbicide-Resistant Soybean and Hybrid Seeds via Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging

Proteomics approach to investigating osmotic stress effects on pistachio

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