Comparative proteome analysis of abdominal adipose tissues between fat and lean broilers

Wu, Chunyan; Liu, Chun-Dong; Wang, Yuxiang; Wei, Na; Wang, Ning; Li, Hui

doi:10.1186/s12953-016-0100-2

Cited by 6 publications

(5 citation statements)

References 47 publications

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“…Phosphorylated HSP27 promotes autophagy and hepatic lipid clearance via autophagy-lysosome pathway in human hepatic cells [ 34 ]. We previously found that HSP27 protein was down-regulated in the abdominal adipose tissue of fat birds [ 12 , 13 ], and here we found that HSP27 was down-regulated in the liver tissue of fat birds at E7 and E12. Taken together, our data strongly suggest that HSP27 might be important for hepatic lipid metabolism in chickens.…”

Section: Discussionsupporting

confidence: 72%

“…However, no transcriptional difference for ApoA-I in the embryonic liver was found between the fat and lean broilers at E17, suggesting the ApoA-I may be regulated at the translational level. We previously examined ApoA-I and its association with fat deposition using genetics, gene expression and proteomics methods in adipose tissues [ 12 , 13 ]. ApoA-I is a major component of high-density lipoprotein (HDL) in the plasma [ 26 ], and can promote cholesterol efflux from peripheral tissues to the liver to keep body cholesterol in balance [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

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Embryonic transcriptome and proteome analyses on hepatic lipid metabolism in chickens divergently selected for abdominal fat content

Wei

Gong

et al. 2018

BMC Genomics

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BackgroundIn avian species, liver is the main site of de novo lipogenesis, and hepatic lipid metabolism relates closely to adipose fat deposition. Using our fat and lean chicken lines of striking differences in abdominal fat content, post-hatch lipid metabolism in both liver and adipose tissues has been studied extensively. However, whether molecular discrepancy for hepatic lipid metabolism exists in chicken embryos remains obscure.ResultsWe performed transcriptome and proteome profiling on chicken livers at five embryonic stages (E7, E12, E14, E17 and E21) between the fat and lean chicken lines. At each stage, 521, 141, 882, 979 and 169 differentially expressed genes were found by the digital gene expression, respectively, which were significantly enriched in the metabolic, PPAR signaling and fatty acid metabolism pathways. Quantitative proteomics analysis found 20 differentially expressed proteins related to lipid metabolism, PPAR signaling, fat digestion and absorption, and oxidative phosphorylation pathways. Combined analysis showed that genes and proteins related to lipid transport (intestinal fatty acid-binding protein, nucleoside diphosphate kinase, and apolipoprotein A-I), lipid clearance (heat shock protein beta-1) and energy metabolism (NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 and succinate dehydrogenase flavoprotein subunit) were significantly differentially expressed between the two lines.ConclusionsFor hepatic lipid metabolism at embryonic stages, molecular differences related to lipid transport, lipid clearance and energy metabolism exist between the fat and lean chicken lines, which might contribute to the striking differences of abdominal fat deposition at post-hatch stages.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4776-9) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Embryonic transcriptome and proteome analyses on hepatic lipid metabolism in chickens divergently selected for abdominal fat content

Wei

Gong

et al. 2018

BMC Genomics

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“…Since 1996, we established two broilers lines based on divergent selection on abdominal fat percentage and plasma very low-density lipoprotein (VLDL) concentration (NEAUHLF) [3], which is an ideal model for studying the molecular basis of adipose tissue growth and development. As a result, we have discovered a number of key genes underlying fat deposition through microarray [4][5] and two-dimensional gel electrophoresis technologies, such as adipocyte fatty acid binding protein (A-FABP) and Apolipoprotein A-I (Apo-AI) [6][7]. However, the molecular mechanism for abdominal fat deposition differences between fat and lean broiler lines remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Integrated transcriptome and proteome analysis reveals potential mechanisms for differential abdominal fat deposition between divergently selected chicken lines

Wang

Leng

Ding

et al. 2021

Journal of Proteomics

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Background: Genetic selection for meat production performance of broilers concomitantly causes excessive abdominal fat deposition, accompanied by several adverse effects, such as the reduction of feed conversion e ciency and reproduction performance. Our previous studies have identi ed important genes regulating chicken fat deposition, using the Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF) as an animal model. However, the molecular mechanism underlying fat deposition differences between fat and lean broilers remains largely unknown.Results: Here, we integrated the transcriptome (RNA-Seq) and quantitative proteome (isobaric tags for relative and absolute quantitation, iTRAQ) pro ling analyses on abdominal fat tissues from NEAUHLF chicken lines. Differentially expressed genes (466 DEGs) and proteins (231 DEPs) were identi ed, and enriched in pathways related to fatty acid metabolism, fatty acid biosynthesis, glycerophospholipid metabolism, and PPAR signaling, and in pathways mainly involved in protein processing, endocytosis and lipid metabolism, respectively. Moreover, several key DEGs and DEPs involved in long-chain fatty acid uptake, in situ lipogenesis (fatty acid and cholesterol synthesis), and lipid droplets accumulation were discovered, and most of them were up-regulated in the fat line, after integrated transcriptome and proteome analysis.Conclusions: Together, our ndings provided a novel insight into abdominal fat content discrepancy between the fat and lean chicken lines.

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“…The deposition of fat in the body has a precise temporal and spatial sequence (Gri n et al 1987). The measurement results show that the sequence of fat deposition sites in broilers is abdominal organs, subcutaneous tissues, muscle bundles, and nally, muscle bers (Wang et al 2009;Wu et al 2016). The high-intensity breeding selection causes the growth speed of the broiler rapidly, almost reaching its physiological limit, leading to a major reduction in the quality of the meat.…”

Section: Discussionmentioning

confidence: 93%

Proteomics Analysis and Identification of Critical Proteins and Network Interactions That Regulate the Specific Deposition of IMF of Jingyuan Chicken

Huang

Zhang

et al. 2020

Preprint

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Background:Improving the production efficiency and quality of broiler chickens has become a hot topic in molecular breeding of the broiler industry. There are several factors, among which intramuscular fat (IMF) content is one of the essential factors determining chicken quality. The chicken carcass weight of 40-50% consisted of the leg muscles and breast muscles, and the research on chicken meat quality was performed on that part. Jingyuan chicken is one of the high-quality local chicken species in China. However, the molecular mechanism of IMF specific deposition in different parts of Jingyuan chicken is still unclear. In order to screen and identify the proteins that regulate IMF specific deposition in different parts of Jingyuan chicken and analyze network interaction, the present study selected 180-day-old Jingyuan chicken as the research material, using proteomic techniques and PRM experimental methods to carry out basic research on leg muscle and breast muscle. Results:Three key signaling pathways and ten essential functional proteins regulating IMF specific deposition of Jingyuan chicken were identified, while the molecular mechanism of the deposition of proteins was explained, and analyzed the network interaction between these key functional proteins. Conclusions:The results of this study are of great scientific value and theoretical significance not only for improving chicken quality but also for molecular breeding of high-quality local chickens.

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Comparative proteome analysis of abdominal adipose tissues between fat and lean broilers

Cited by 6 publications

References 47 publications

Embryonic transcriptome and proteome analyses on hepatic lipid metabolism in chickens divergently selected for abdominal fat content

Embryonic transcriptome and proteome analyses on hepatic lipid metabolism in chickens divergently selected for abdominal fat content

Integrated transcriptome and proteome analysis reveals potential mechanisms for differential abdominal fat deposition between divergently selected chicken lines

Proteomics Analysis and Identification of Critical Proteins and Network Interactions That Regulate the Specific Deposition of IMF of Jingyuan Chicken

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