Comparative proteome analysis of glutenin synthesis and accumulation in developing grains between superior and poor quality bread wheat cultivars

Abstract

The high accumulation level of HMW-GSs and LMW-GSs as well as 1Bx13 + 1By16 and 1Dx4 + 1Dy12 subunits contributed to the superior gluten quality of Jimai 20. Two highly expressed and 16 specifically expressed LMW glutenin subunits in Jimain 20 had positive effects on dough quality, while 17 specifically expressed subunits in Zhoumai 16 and Jing 411 appeared to have negative effects on gluten quality.

Synthesis and accumulation of glutenin subunits during grain development in bread wheat as revealed by reversed-phase ultra-performance liquid chromatography (RP-UPLC)

“…Previous reports demonstrated some differences for the synthesis and accumulation of storage proteins, possibly resulted from different materials used and their growth environments. HMW-GS, LMW-GS and gliadins occurred concurrently at as early as 7 DPA (Gupta et al 1996;Liu et al 2012). But in the report of Panozzo et al (2001), LMW-GS was synthesized later than HMW-GS and negligible concentrations could be detected before 14 DPA.…”

“…Grain samples were ground into fine powder using a mortar and glutenin proteins were extracted according to Liu et al (2012). All samples were used for SDS-PAGE and RP-UPLC analyses within 24 h of extraction.…”

“…To date, various methods have been developed to study the synthesis and accumulation patterns of wheat storage proteins, including the mostly used traditional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and reversed-phase (RP) and size-exclusion (SE) high-performance liquid chromatography (HPLC) (Gupta et al 1993;Liu et al 2012). Glutenins can be detected from as early as 7 days post anthesis (DPA) by using SE-HPLC (Gupta et al 1993) and RP-HPLC (Liu et al 2012).…”

“…Glutenins can be detected from as early as 7 days post anthesis (DPA) by using SE-HPLC (Gupta et al 1993) and RP-HPLC (Liu et al 2012). SDS-PAGE can detect glutenin synthesis at 7-14 DPA (Ng et al 1990;Liu et al 2012) depend on the materials used and their growth environments.…”

“…Glutenins can be detected from as early as 7 days post anthesis (DPA) by using SE-HPLC (Gupta et al 1993) and RP-HPLC (Liu et al 2012). SDS-PAGE can detect glutenin synthesis at 7-14 DPA (Ng et al 1990;Liu et al 2012) depend on the materials used and their growth environments. Glutenins of the cultivars with 1Dx5 + 1Dy10 subunits were found to accumulate larger polymers more quickly than with 1Dx2 + 1Dy12 at early grain development stage (Gupta et al 1993).…”