Comparative proteome analysis reveals pathogen specific outer membrane proteins of Leptospira

Abstract

Proteomes of pathogenic Leptospira interrogans and L. borgpetersenii and the saprophytic L. biflexa were filtered through computational tools to identify Outer Membrane Proteins (OMPs) that satisfy the required biophysical parameters for their presence on the outer membrane. A total of 133, 130, and 144 OMPs were identified in L. interrogans, L. borgpetersenii, and L. biflexa, respectively, which forms approximately 4% of proteomes. A holistic analysis of transporting and pathogenic characteristics of OMPs together with Clusters of Orthologous Groups (COGs) among the OMPs and their distribution across 3 species was made and put forward a set of 21 candidate OMPs specific to pathogenic leptospires. It is also found that proteins homologous to the candidate OMPs were also present in other pathogenic species of leptospires. Six OMPs from L. interrogans and 2 from L. borgpetersenii observed to have similar COGs while those were not found in any intermediate or saprophytic forms. These OMPs appears to have role in infection and pathogenesis and useful for anti-leptospiral strategies.

Enhancement of clinical signs in C3H/HeJ mice vaccinated with a highly immunogenicLeptospiramethyl-accepting chemotaxis protein following challenge

“…Continuous improvements in bioinformatics tools provide the opportunity to identify proteins based on their potential structures, biological function, and capacity to induce protective humoral and cellular immune responses (23)(24)(25). The in-silico prediction of potential immunogenic Leptospira proteins can be used to effectively identify target molecules that can generate both humoral and/or cell mediated immune responses (26)(27)(28)(29).…”

“…Since many of the vaccine candidates studied have not provided effective and reproducible protection against acute and chronic disease (14), new approaches to select Leptospira vaccine candidates are needed. Continuous improvements in bioinformatics tools provide the opportunity to identify proteins based on their potential structures, biological function, and capacity to induce protective humoral and cellular immune responses (23-25). The in-silico prediction of potential immunogenic Leptospira proteins can be used to effectively identify target molecules that can generate both humoral and/or cell mediated immune responses (26-29).…”

Surface?proteomics?of?Leptospira?interrogans?serovar?Pomona

“…13). outer membrane was predicted by all 3 tools (OM3), outer membrane (OM), periplasm Previous studies in pathogenic Leptospira focused on OMPs that are highly abundant proteins (14,15,67,70), host ligand-binding proteins (94), and highly antigenic and conserved proteins (96,97). Reverse vaccinology has been used for highthroughput screening of leptospiral OMPs/PSEs as new vaccine candidates.…”