Comparative proteomic analysis in pea treated with microbial consortia of beneficial microbes reveals changes in the protein network to enhance resistance against Sclerotinia sclerotiorum

Chrysophanol is involved in the biofertilization and biocontrol activities of Trichoderma

“…They proposed that the effect of defense gene expression on the resistance of Trichoderma-treated tomato plants against B. cinerea infection is complex and involves both systemic acquired resistance (SAR) and induced systemic resistance (ISR), probably with the salicylic acid (SA)-responsive genes contributing to the defense reactions at the early stage (24 h post-infection), and the jasmonic acid (JA)-responsive genes being more effective at the later stage (48 h post-infection). Similar phenomenon were also reported by Jain et al [27] who pretreated pea with T. harzianum followed by a challenge with Sclerotinia sclerotiorum and observed that increments in protein production were downregulated compared with control plants. Moreover, they mentioned that plants treated with beneficial microorganisms revealed an increase in their primary metabolism, such as photosynthesis, upon pathogen infection.…”

Insight into the Interaction between Plants and Associated FluorescentPseudomonasspp.

“…Recent studies by our group on pea plant treated with consortia of fluorescent P. aeruginosa, B. subtilis, and T. harzianum and challenged with S. sclerotiorum under greenhouse conditions showed decrease in plant mortality and increase in activities of defense-related and antioxidant enzymes and phenols and suppression of oxalic acid induced cell death [6,12,62,67]. Also, proteome level study using 2D gel electrophoresis on the same system showed 30 differential protein spots, 25 of which were identified by MALDI TOF MS/MS to be involved in photosynthesis, respiration, disease resistance, and stress alleviation [63]. Such reports support the beneficial effect of fluorescent Pseudomonas in consortia mode with other microorganisms in root colonization, increasing plant growth and activities of defense-related enzymes, and induction of phenylpropanoid pathway and therefore it may also be expected to alter the production and composition of secondary metabolites in plants [10].…”

“…Fluorescent P. aeruginosa PJHU15 was found to produce IAA and solubilise phosphate in vitro using plate assays [62]. The same fluorescent P. aeruginosa in consortium with Trichoderma harzianum and Bacillus subtilis also showed improved plant health, induction of systemic resistance, and proteome level changes upon challenge with Sclerotinia sclerotiorum [6,12,62,63]. The same combination of microbes also modulated nutritional and antioxidant quality of pea seeds and pericarp [64].…”

Bacillus: A Biological Tool for Crop Improvement through Bio-Molecular Changes in Adverse Environments

“…Bacillus spp. mitigate pathogen-induced biotic stress via physiological changes (Table 2 ) in the photosynthetic and respiratory pathways and the regulation of carbohydrate, phenyl-propanoid and N metabolism and defense-related proteins in diseased plants (Jain et al, 2015 ). Gene expression patterns in plants are also altered during infection by pathogenic fungi, and a number of dependent genes are activated to protect the plant from biotic stresses.…”