Comparative Proteomic Analysis of Antibiotic Resistant Strains of Vibrio CholeraeIsolated from Marine Fish

Abstract

Cholera is an infectious disease prevalent in developing countries like India. Poor sanitation conditions in slums causes release of contaminated water in the environment. Naturally inhabitant organisms become resistant to the antibiotics due to pharma industrial effluent, domestic waste and usage of antibiotics in aquaculture. One such natural inhabitant bacteria present in the marine fishes is Vibrio cholerae. In our study, we have isolated Vibrio cholerae from marine fish to show flow of antimicrobial resistance proteins in the aquatic environment using one of the commonly consumed fish (Rastrelliger kanagurta) in India. Biochemical tests were used to characterize the organism. In order to study drug resistance, Minimum Inhibitory Concentration (MIC) breakpoint assay was employed for common antibiotics like Ampicillin, Kanamycin, Streptomycin and Chloramphenicol. The growth pattern was studied by optical density and dry cell mass of the isolate grown in presence and absence of each antibiotics as well as in combination. Effect of these antibiotics and mechanism of cause of resistance was studies from the proteomic point of view using SDS-PAGE based comparative studies that yielded in increased and reduced expression of various proteins in the whole cell protein analysis, prominently under multi-drug treatment. Comparative analysis of these proteins with enlisted proteins in Uniport suggests the drug-resistance pattern in Vibrio cholerae.