Comparative proteomic analysis of biofilm and planktonic cells of <i>Neisseria meningitidis</i>

Alen, Tessa van; Claus, Heike; Zahedi, René P.; Groh, Janos; Blazyca, Heinrich; Lappann, Martin; Sickmann, Albert; Vogel, Ulrich

doi:10.1002/pmic.201000267

Cited by 25 publications

(23 citation statements)

References 53 publications

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“…These genes include oxyR, prx, gor (24), and trxB (34) in gonococcus and mntC (13,35) in meningococcus and gonococcus. Thioredoxins are oxidoreductase enzymes that play a role in reactive oxygen species response.…”

Section: Discussionmentioning

confidence: 99%

“…Several recent studies have attempted to identify genes involved in the colonization of either gonococcus or meningococcus by transcriptomic or proteomic comparison of biofilm versus planktonic growth. All of these studies have been conducted on inert substratum under a continuous flow of medium (13)(14)(15)30). In gonococcus, genes found to be highly upregulated in biofilms included nitrite reductase gene (aniA) and nitric oxide reductase gene (norB), which are both part of the NarP regulon (15).…”

Section: Discussionmentioning

confidence: 99%

“…More recently, it has been evidenced that wild-type (WT) encapsulated N. meningitidis was able to form biofilms on human tissue culture cells in a flow chamber (8). In addition, proteomic and transcriptomic studies comparing planktonic versus biofilm grown meningococcus or gonococcus over inert surface have highlighted the role of enzymes involved in denitrification and adaptation to reactive oxygen species (13)(14)(15).…”

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confidence: 99%

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Identification of Genes Involved in Neisseria meningitidis Colonization

et al. 2013

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Neisseria meningitidis is a worldwide cause of meningitis and septicemia leading at least to 50,000 deaths every year. Nevertheless, N. meningitidis is also a commensal bacterium that asymptomatically colonizes the epithelial cells of the nasopharynx of 10 to 30% of healthy individuals. Occasionally, N. meningitidis crosses the nasopharyngeal barrier and enters the bloodstream. During bacteremia, N. meningitidis may adhere to endothelial cells of brain vessels and invade meninges. To identify the genes required for meningococcal host colonization, we screened a signature-tagged transposon mutagenesis library using an innovative in vitro colonization model in order to identify mutants displaying decreased capacity to colonize human epithelial cells. Approximately 1,600 defined insertion mutants of invasive serogroup C strain NEM8013 were screened. Candidate mutants were tested individually for quantification of bacterial biomass with confocal microscope and COMSTAT software. Five mutants were demonstrated to exhibit significantly decreased colonization ability. The identified genes, including narP and estD, appeared to be involved in adaptation to hypoxic conditions and stress resistance. Interestingly, the genes fadD1, nnrS, and NMV_2034 (encoding a putative thioredoxin), prior to this study, had not been shown to be involved in colonization. Therefore, we provide here insights into the meningococcal functions necessary for the bacterium to adapt to growth on host cells.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Identification of Genes Involved in Neisseria meningitidis Colonization

et al. 2013

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“…Of note, expression of NMB0586 (designated MntC by van Alen et al [63] or ZnuA by Stork et al [59]) and NMB1475 was upregulated in biofilms, and deletion of nmb0586 reduced biofilm formation (63). Extenuated biofilm formation has previously also been seen upon deletion of znuA in gonococci (30) and nmb0587 (znuB) in E. coli (19).…”

Section: Zinc-responsive Regulon Of Meningococcimentioning

confidence: 99%

The Zinc-Responsive Regulon of Neisseria meningitidis Comprises 17 Genes under Control of a Zur Element

et al. 2012

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Zinc is a bivalent cation essential for bacterial growth and metabolism. The human pathogenNeisseria meningitidisexpresses a homologue of the Zinc uptake regulator Zur, which has been postulated to repress the putative zinc uptake protein ZnuD. In this study, we elucidated the transcriptome of meningococci in response to zinc by microarrays and quantitative real-time PCR (qRT-PCR). We identified 15 genes that were repressed and two genes that were activated upon zinc addition. All transcription units (genes and operons) harbored a putative Zur binding motif in their promoter regions. A meningococcal Zur binding consensus motif (Zur box) was deducedin silico, which harbors a conserved central palindrome consisting of hexameric inverted repeats separated by three nucleotides (TGTTATDNHATAACA).In vitrobinding of recombinant meningococcal Zur to this Zur box was shown for the first time using electrophoretic mobility shift assays. Zur binding to DNA depended specifically on the presence of zinc and was sensitive to mutations in the palindromic sequence. The Zur regulon among genes of unknown function comprised genes involved in zinc uptake, tRNA modification, and ribosomal assembly. In summary, this is the first study of the transcriptional response to zinc in meningococci.

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“…Oxidative DNA damage is increased and is associated with the increased genetic mutability in several species (Boles and Singh 2008;Ryder et al 2012;Allegrucci and Sauer 2007) (Arce Miranda et al 2011). Although discrepant results have been reported, several transcriptomic and proteomic studies reveal an upregulated response to oxidative stress and expression of antioxidant defenses (Resch et al 2005;Patrauchan et al 2007;van Alen et al 2010;Kim et al 2006;Tremoulet et al 2002). This oxidative stress-rich environment can promote antibiotic resistance and tolerance to biofilm cells through several mechanisms.…”

Section: Oxidative Stressmentioning

confidence: 94%

Antibiotic Resistance and Tolerance in Bacterial Biofilms

McKay

Nguyen

2014

Handbook of Antimicrobial Resistance

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Bacteria can grow as multicellular communities called biofilms, and this sessile lifestyle is distinct from planktonic growth. While microbial biofilms are ubiquitous in the natural and industrial environment, their importance in human infections has only been fully recognized in the past few decades. Biofilm-associated bacteria typically cause subacute and chronic infections. Many bacterial pathogens, such as Staphylococcus aureus, readily form biofilms, and Pseudomonas aeruginosa, which causes chronic airway infections in patients with cystic fibrosis, is an important model organism for biofilm studies. They are clinically significant due to their persistence despite sustained antimicrobial treatments and adequate host defenses. Biofilm bacteria are highly resistant to a wide range of antimicrobial compounds and disinfectants, and the mechanisms underlying this resistance are likely multifactorial. This chapter will review the cellular processes and pathways implicated in antibiotic resistance and tolerance of bacterial biofilms.

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Comparative proteomic analysis of biofilm and planktonic cells of Neisseria meningitidis

Cited by 25 publications

References 53 publications

Identification of Genes Involved in Neisseria meningitidis Colonization

Identification of Genes Involved in Neisseria meningitidis Colonization

The Zinc-Responsive Regulon of Neisseria meningitidis Comprises 17 Genes under Control of a Zur Element

Antibiotic Resistance and Tolerance in Bacterial Biofilms

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