Comparative proteomic analysis of canola leaves under salinity stress

Bandehagh, Ali; Salekdeh, Ghasem Hosseini; Toorchi, Mahmoud; Mohammadi, Seyed Abolghasem; Komatsu, Setsuko

doi:10.1002/pmic.201000564

Cited by 107 publications

(102 citation statements)

References 42 publications

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“…The increase in soluble protein contents in rice plants by exogenous application of plant growth regulators under water stress may be due to their possible involvement in water stress adaptation process (Tuna et al, 2008). The plants produce proteins which are involved in decontamination of free radicals and thus play important role in plants adaptation under stress conditions (Witzel et al, 2009;Bandehagh et al, 2011). It is well documented that the cellular proteins contents of plants pass through variations under stress conditions (Guo and Song, 2009;Mohammadkhani and Heidari, 2008).…”

Section: Discussionmentioning

confidence: 99%

Exogenously Applied Gibberellic Acid, Indole Acetic Acid and Kinetin as Potential Regulators of Source-Sink Relationship, Physiological and Yield Attributes in Rice (Oryza sativa) Genotypes under Water Deficit Conditions

Khan¹,

Gurmani²,

Jalaluddin³

et al. 2015

IJAB

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To cite this paper: Khan, S.U., A.R. Gurmani, Jalal-Ud-Din, A. Qayyum, K.S. Abbasi, M. Liaquat and Z. Ahmad, 2016. Exogenously applied gibberellic acid, indole acetic acid and kinetin as potential regulators of source-sink relationship, physiological and yield attributes in rice (Oryza sativa) genotypes under water deficit conditions. AbstractRice is one of the important cereal crops drastically affected by water scarcity in rice ecosystems. In a pot study, the impact of externally applied gibberellic acid (GA3), indole acetic acid (IAA) and kinetin was investigated on assimilates mobilization and yield parameters of rice under water stress. Twenty five days seedlings of two rice genotypes KS-133 and Basmati-2000 were transplanted in soil filled pots and sprayed with GA3, IAA and kinetin each of 10 -5 M concentration at panicle initiation stage. Water stress was imposed to plants 80 days after transplanting for 10 days. Water stress (p≤ 0.05) reduced leaf chlorophyll content and yield of rice but increased the concentration of proline, leaf soluble protein and enhanced mobility of assimilates from leaves to grains. Application of GA3 and kinetin significantly (p≤ 0.05) increased chlorophyll content, growth and yield attributes in both genotypes under water stress conditions. However, IAA application did not improve the grain yield in rice genotypes under water stress. The positive impact of GA3 and kinetin spray was imitated in the form of enhanced solute accumulation; enhanced growth and greater grain assimilate deposition. This may be concluded that GA3 and kinetin can be applied exogenously prior to flower initiation in rice to reduce panicle sterility by increasing assimilates mobility under water deficit for optimal economic yield.

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Section: Discussionmentioning

confidence: 99%

Exogenously Applied Gibberellic Acid, Indole Acetic Acid and Kinetin as Potential Regulators of Source-Sink Relationship, Physiological and Yield Attributes in Rice (Oryza sativa) Genotypes under Water Deficit Conditions

Khan¹,

Gurmani²,

Jalaluddin³

et al. 2015

IJAB

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“…It also makes the investigation of dynamic expression of proteins possible (Guo et al, 2012). Bandehagh et al (2011), studied salt tolerance in canola by extracting proteins from seedling´s leaves. The results indicated that Na accumulation in sensitive cultivars is higher than tolerant cultivars.…”

Section: Discussionmentioning

confidence: 99%

“…There, reduction in abundance of RubisCo large/small subunit, chloroplast precursor (OEE), Oxygen-evolving enhancer protein 2, carbonic anhydrases as well as Rubisco's activity leads to short assimilation. Fall in assimilation has been reported for many glycophyte plants subjected to salinity stress (Aghaei, 2008; Pang et al, 2010;Sobhanian et al, 2010;Chatlopadhyay 2011 andBandehagh et al, 2011). However, in some sensitive plants, including durum wheat, an increase in activity of RubisCo is thought to be a response rendered to support the carboxylase activities of RubisCo (Caruso et al, 2008).…”

Section: Protein Identificationmentioning

confidence: 99%

“…Proteins with more than one occurrence have previously been 364 detected by other researchers (Caruso et al, 2008;Wang et al 2008;Gao et al, 2011 andBandehagh et al, 2011) the reason for which has been explained as existing protein isoforms, posttranslation modifications, alternative splicing of mRNA and protein breakage. Post-translation modification such as glycosylation and phosphorylation can change molecular weight and isoelectric focusing of the proteins (Caruso et al, 2008).…”

Section: Protein Identificationmentioning

confidence: 99%

See 1 more Smart Citation

Canola Seedling Response to NaCl Stress – a Proteomic Approach

Alagoz

Toorchi

Bandehagh

2016

Not Bot Horti Agrobo

Self Cite

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Salinity is a major abiotic stress worldwide, which causes a tremendous loss in the quality and quantity of agricultural products. In order to identify defense mechanisms to salinity-induced stress, a two-dimensional electrophoresis approach was used to investigate proteins achieved from canola cultivar 'Option 500' seedlings, after plants had been treated with NaCl. The 300 mmol NaCl treatment caused a significant decrease in fresh and dry weight as well as plant height and increment of proline content compared with control group. There were no significant changes in the quality of catalase and peroxidase activity. Out of 110 repeatable protein spots, 44 were registering change, according to induction factor index. 7 spots were recognized significant at 1% probability level statistically. These proteins were involved in energy production, ROS scavenging and suppressor, and those involved in photosynthesis. Production of chloroplastic fructose-bisphosphate aldolase enzyme along with carbohydrate metabolisms of Kelvin cycle and glycolysis increases under salinity condition. The amount of Cu/Zn SOD in chloroplast reduces in response to salinity which results from an increase in OH -radicals. Expression of photosynthesis proteins have been reduced probably as a consequence of the drop in osmotic pressure resulted from water scarcity and stomata closure, which reduces the availability of Co2.

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“…Gadallah [18] found that salinity decreased the soluble proteins content of Vicia faba L. exposed to salt stress. Under salinity the plants produce stress-responsive proteins that are involved in detoxification of ROS and thus play a role in adaptation to stress [6]. According to the plant species or cultivar the stress conditions resulted in changes in soluble protein contents due to cell structural modifications [49].…”

Section: Acta Biologica Hungarica 66 2015mentioning

confidence: 99%

Morphological and physiological changes in esterase and lipid peroxidation of two bean cultivars pre-soaked with potassium nitrate under salt stress

Shaddad

El-Baki

Mostafa

et al. 2015

Acta Biologica Hungarica

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Two broad bean cultivars (Vicia faba CV Nobaria3 and Vicia faba CV Sakha3) were obtained from Mallwi Agriculture Research Center, El Minia Governorate, Egypt. The seeds were divided into two groups, the first group soaked with distilled water, while the second group were soaked with 3 mM KNO 3 , respectively, for 4 hours. Seeds were sown and left to grow for 3 weeks then treated with different concentrations of NaCl (0.0, 40, 80, 120 and 160 mM) by top irrigation, then they left to grow further for 65 days from sowing. Plant samples were collected for some measurements: leaf area, plant height, root length, fresh and dry weight, photosynthetic pigments, carotenoids, soluble sugars, soluble proteins, total free amino acids, esterase enzyme, as well as MDA (malondialdehyde) content. Salinity reduced both fresh and dry weight in two broad bean cultivars, this reduction were more pronounced in Sakha3 than Nobaria3. Seed pre-soaking with KNO 3 resulted in enhancement of fresh and dry weight production in both cultivars especially at 40 mM NaCl. Photosynthetic pigments were substantially affected by salt treatment while the carotenoids were increased, seed pre-soaking with KNO 3 improved these components. The soluble sugars, amino acids as well as soluble proteins showed various responses with increasing salinity in the cultivars, seed pre-soaking with KNO 3 has improved these parameters to some extent. The shoots of two cultivars exhibited significant accumulation of MDA, compared to roots exposed to the highest salinity levels. Pre-soaking seeds with KNO 3 did not improve MDA in shoots but enhanced it in roots, however, in most cases still lower than the absolute control. The assessment of the esterase isozyme profiles on 7.5% native polyacrylamide gel revealed the presence of 13 isoforms in two faba bean plants in response to KNO 3 pre-soaking and treatments with different concentrations of NaCl.

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Comparative proteomic analysis of canola leaves under salinity stress

Cited by 107 publications

References 42 publications

Exogenously Applied Gibberellic Acid, Indole Acetic Acid and Kinetin as Potential Regulators of Source-Sink Relationship, Physiological and Yield Attributes in Rice (Oryza sativa) Genotypes under Water Deficit Conditions

Exogenously Applied Gibberellic Acid, Indole Acetic Acid and Kinetin as Potential Regulators of Source-Sink Relationship, Physiological and Yield Attributes in Rice (Oryza sativa) Genotypes under Water Deficit Conditions

Canola Seedling Response to NaCl Stress – a Proteomic Approach

Morphological and physiological changes in esterase and lipid peroxidation of two bean cultivars pre-soaked with potassium nitrate under salt stress

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