Comparative proteomic analysis of hepatopancreas in Macrobrachium rosenbergii responded to Poly (I:C)

Saray, Pheng; Roytrakul, Sittiruk; Pangeson, Tanapat; Phetrungnapha, Amnat

doi:10.1016/j.fsi.2018.02.008

Cited by 10 publications

(3 citation statements)

References 59 publications

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“…This could be due to increased energy requirements by enzymes involved in BCAA/glutathione synthesis and transsulphuration pathways, glycolysis and the cellular nicotinamide adenine dinucleotide phosphate oxidase complex immune response. In aquatic invertebrates, poly (I:C) and viral infections enhanced energy requirements (Li et al ., ; Saray et al ., ). These results revealed that poly (I:C) induces Warburg‐like metabolic effects in treated fish, similar to the conditions observed during intracellular viral replication (Eisenreich et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Metabolic and immune responses of Chinook salmon (Oncorhynchus tshawytscha) smolts to a short‐term poly (I:C) challenge

Lulijwa

Mérien

Burdass

et al. 2020

Journal of Fish Biology

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Polyinosinic:polycytidylic acid [poly (I:C)] was administered in vivo to Chinook salmon (Oncorhynchus tshawytscha) post‐smolts to determine the immune responses on haematological and cellular functional parameters, including spleen (SP), head kidney (HK) and red blood cell (RBC) cytokine expression, as well as serum metabolomics. Poly (I:C) in vivo (24 h exposure) did not affect fish haematological parameters, leucocyte phagocytic activity and phagocytic index, reactive oxygen species and nitric oxide production. Gas chromatography–mass spectrometry–based metabolomics revealed that poly (I:C) significantly altered the serum biochemistry profile of 25 metabolites. Metabolites involved in the branched‐chain amino acid/glutathione and transsulphuration pathways and phospholipid metabolism accumulated in poly (I:C)–treated fish, whereas those involved in the glycolytic and energy metabolism pathways were downregulated. At cytokine transcript level, poly (I:C) induced a significant upregulation of antiviral ifnγ in HK and Mx1 protein in HK, SP and RBCs. This study provides evidence for poly (I:C)–induced, immune‐related biomarkers at metabolic and molecular levels in farmed O. tshawytscha in vivo. These findings provide insights into short‐term effects of poly (I:C) at haematological, innate and adaptive immunity and metabolic levels, setting the stage for future studies.

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Section: Discussionmentioning

confidence: 97%

Metabolic and immune responses of Chinook salmon (Oncorhynchus tshawytscha) smolts to a short‐term poly (I:C) challenge

Lulijwa

Mérien

Burdass

et al. 2020

Journal of Fish Biology

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“…In recent years, the application of proteomics analysis in aquaculture has been heating up ( 17 , 40 ). Based on KEGG enrichment analyses, the present study identified that TTO significantly regulated fatty acid biosynthesis and metabolism and glycerophospholipid metabolism pathways.…”

Section: Discussionmentioning

confidence: 99%

“…The label-free quantification proteomic approach allows simultaneous identification and quantification and applies to samples from any source ( 14 – 16 ). Existing studies have used proteomics sequencing to screen the differentially expressed proteins (DEPs) in the hepatopancreas and then explore the interrelationships among DEPs on M. rosenbergii and Eriocheir sinensis after feed additives intervention ( 17 , 18 ), which demonstrates the feasibility of this technique in spineless animals.…”

Section: Introductionmentioning

confidence: 99%

Comparative Proteomic Analysis Revealed the Mechanism of Tea Tree Oil Targeting Lipid Metabolism and Antioxidant System to Protect Hepatopancreatic Health in Macrobrachium rosenbergii

Sun

Zheng

et al. 2022

Front. Immunol.

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Tea tree oil (TTO) is a pure natural plant essential oil. The studies evaluated the hepatopancreas lipid metabolism and antioxidant efficacy of Macrobrachium rosenbergii fed with 0 (CT group) and 100 mg/kg TTO (TT group) by label-free quantification proteomic analysis. Compared to the CT group, the TT group improved growth performance and increased the survival rate after stress. Dietary TTO also decreased hemolymph AST and ALT activities and decreased hepatopancreatic vacuolation. At the same time, hepatopancreas lipids droplets and hemolymph lipids (TG, TC, LDL-C) were decreased, and the peroxidation products content (MDA, LPO, 4-HNE) was also decreased. In addition, the levels of hepatopancreas antioxidant enzymes (T-AOC, CAT, and SOD) were increased in the TT group. With proteomic analysis, a total of 151 differentially expressed proteins (DEPs) (99 up-regulated and 52 down-regulated) were identified in the hepatopancreas. Kyoto Encyclopedia of Genes and Genomes (KEGG) and protein-protein interaction analysis showed that the 16 DEPs have interactions, which are mainly involved in the pathways related to lipid metabolism (fatty acid biosynthesis, fatty acid metabolism, glycerophospholipid metabolism) and redox reaction (cytochrome P450 enzyme systems). Furthermore, the mRNA expression of 15 proteins followed the proteomic analysis with qRT-PCR validation. Pearson correlation analysis showed that fatty acids and glycerophospholipid metabolism-related proteins were highly correlated to peroxide content, glycerophospholipid metabolism, and cytochrome P450 system-related proteins (CYP1A1, GSTT1, GPX4) were highly correlated to AST and ALT. Additionally, GPX4 is closely related to peroxide content and antioxidant enzyme activity. Our results revealed that TTO plays a protective role in the hepatopancreas targeting the critical enzymes and antioxidant reactions in lipid metabolism. Provides a new perspective to elucidate the action path of TTO in protecting invertebrate hepatopancreas, highlights the influence of lipid metabolism on hepatopancreas health and the interaction between lipid metabolism and antioxidant system in the regulation of TTO.

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Polyinosinic:polycytidylic acid in vivo enhances Chinook salmon (Oncorhynchus tshawytscha) immunity and alters the fish metabolome

et al. 2020

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Comparative proteomic analysis of hepatopancreas in Macrobrachium rosenbergii responded to Poly (I:C)

Cited by 10 publications

References 59 publications

Metabolic and immune responses of Chinook salmon (Oncorhynchus tshawytscha) smolts to a short‐term poly (I:C) challenge

Metabolic and immune responses of Chinook salmon (Oncorhynchus tshawytscha) smolts to a short‐term poly (I:C) challenge

Comparative Proteomic Analysis Revealed the Mechanism of Tea Tree Oil Targeting Lipid Metabolism and Antioxidant System to Protect Hepatopancreatic Health in Macrobrachium rosenbergii

Polyinosinic:polycytidylic acid in vivo enhances Chinook salmon (Oncorhynchus tshawytscha) immunity and alters the fish metabolome

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