Comparative proteomic analysis of Arabidopsis thaliana roots between wild type and its salt-tolerant mutant

Abstract

Two-dimensional electrophoresis (2-DE) showed the variation expression of Arabidopsis thaliana root proteins between wild type and its salt-tolerant mutant obtained from cobalt-60 g ray radiation. Forty-six differential root protein spots were reproducibly presented on 2-DE maps, and 29 spots were identified by matrix assisted laser desorption ionization-time of flight/time of flight mass spectrometry (MS). Fifteen protein spots corresponding to 10 proteins, and 14 protein spots corresponding to 9 proteins were constitutively up-regulated and downregulated in the salt-tolerant mutant root. Bioinformatic analysis indicated that those differential proteins might be involved in the regulation of redox homeostasis, nucleotide metabolism, signal transduction, stress response and defense, carbohydrate metabolism, and cell wall metabolism. Peroxidase 22 might be a versatile enzyme and might play dual roles in both cell wall metabolism and regulation of redox homeostasis. Our work provides not only new insights into salt-responsive proteins in root, but also the potential salt-tolerant targets for further dissection of molecular mechanism adapted by plants during salt stress.

In-depth investigation on abiotic stress-responsive differentially expressed genes in Arabidopsis roots through GEO database

“…The analysis of PPI interaction networks was performed by the online STRING (search tool for recurring instances of neighboring genes) database (Version 11.0, released January 19, 2019) (http://string-db.org/). Growth conditions and harvest of Arabidopsis root According to previous publications (Guo et al 2014a;Kilian et al 2007), the ecotype Col-0 Arabidopsis seeds were germinated on the normal medium plate with 22/20°C day/night, 8/16 h light/dark cycle, 60 μmol•m −2 s −1 light intensity. After 7 days, the A. thaliana seedlings separately underwent the following stresses: the cold-stress condition (8°C), the osmotic stress condition (200 mmol•L −1 mannitol), the salt stress condition (150 mmol•L −1 NaCl), the wounding stress condition by punctuation of the leaves with needles, the drought stress condition by removing the lid of dish and exposing to a stream of air for 15 min, the UV stress condition by removing the lid of dish and irradiated with UV light for 15 min, the heat stress Figure 1.…”

“…In our previous work, we focused on the salt-responsive molecules using A. thaliana as the model plant. The results indicated the differential molecules were involved in cell wall metabolisms and reactive oxygen species (ROS) scavenging (Guo et al 2014a). More importantly, the complicated cross-tolerances to differential stresses such as osmosis, water deprivation, heat, UV-light and wounding has been shown in A. thaliana (Guo et al 2019).…”

Integrated bioinformatics analysis reveals the response of Arabidopsis roots to salt stress through multiple gene expression omnibus datasets

“…According to the previous publication (Guo et al 2014b), the ecotype Col-0 Arabidopsis thaliana seeds were germinated on the normal medium plate with 22/20°C day/night, 8/16 h light/dark cycle, 60 μmol•m-2s-1 light intensity. After 7 days,…”

“…In order to demonstrate the mechanisms of saltresponse, many researches have been performed with the model plant Arabidopsis (Guo et al 2019). Two-dimensional electrophoresis has been used to discover the differential Arabidopsis root proteins between wild type and salt-tolerance mutant, which identified 19 differential proteins related to signal transduction, redox homeostasis, and cell wall metabolism (Guo et al 2014b). Furthermore, phosphorylation variations of proteins from Arabidopsis root under salt stress also have been determined by Pro-Q Diamond stain.…”

Role of Proteomics in Crop Stress Tolerance

“… Guo et al (2013) showed that 17 unique proteins differentially changed in abundance in response to NaCl in Arabidopsis roots. These identified proteins were believed to be involved in binding catalysts, signal transduction, transport, metabolism of cell wall and energy, and ROS scavenging and defense.…”