Comparative Proteomic Analysis of <i>Arabidopsis</i> Mature Pollen and Germinated Pollen

Zou, Junjie; Song, Lian-Fen; Zhang, Wenzheng; Wang, Yi; Ruan, Songlin; Wu, Weihua

doi:10.1111/j.1744-7909.2009.00823.x

Cited by 73 publications

(58 citation statements)

References 65 publications

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“…However, the fern spore germination happens in the medium, therefore, it is somewhat more complex and affected by various environmental factors, such as light, gravity, calcium, phytohormone, and temperature (6). The protein functional skew in fern spores and pollen grains revealed from this study and recent proteomics studies on germinating pollen from kiwifruit (Actinidia deliciosa) (71,72), Nicotiana tabacum (73), rice (Oryza sativa) (26), Brassica napus (74), Pinus strobus (75), Picea meyeri (76,77), Picea wilsonii (78) and Arabidopsis (79,80) presented new evidence for the differentiation of fern spores and spermatophyte pollen (Fig. 10).…”

Section: Heterotrophic and Autotrophic Metabolisms Are Active Inmentioning

confidence: 72%

“…No photosynthetic proteins were found in pollen. This highlights that the remodeling of pollen tube cell wall, the loosening and hydrolyzing stigma surface cell and transmitting track of pistil, The data of germinating pollen were summarized and reclassified based on pollen proteomic studies on Oryza sativa (26), Actinidia deliciosa (71,72), Nicotiana tabacum (73), Brassica napus (74), Pinus strobus (75), Picea meyeri (76,77), Picea wilsonii (78), and Arabidopsis thaliana (79,80). The y axis represents the reclassified functional categories, and the x axis shows the percentage of identified proteins.…”

Section: Heterotrophic and Autotrophic Metabolisms Are Active Inmentioning

confidence: 99%

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Cytological and Proteomic Analyses of Osmunda cinnamomea Germinating Spores Reveal Characteristics of Fern Spore Germination and Rhizoid Tip Growth*

Suo

Zhao

Zhang

et al. 2015

Molecular & Cellular Proteomics

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Fern spore is a good single-cell model for studying the sophisticated molecular networks in asymmetric cell division, differentiation, and polar growth. Osmunda cinnamomea L. var. asiatica is one of the oldest fern species with typical separate-growing trophophyll and sporophyll. The chlorophyllous spores generated from sporophyll can germinate without dormancy. In this study, the spore ultrastructure, antioxidant enzyme activities, as well as protein and gene expression patterns were analyzed in the course of spore germination at five typical stages (i.e. mature spores, rehydrated spores, double-celled spores, germinated spores, and spores with protonemal cells). Proteomic analysis revealed 113 differentially expressed proteins, which were mainly involved in photosynthesis, reserve mobilization, energy supplying, protein synthesis and turnover, reactive oxygen species scavenging, signaling, and cell structure modulation. The presence of multiple proteoforms of 25 differentially expressed proteins implies that post-translational modification may play important roles in spore germination. The dynamic patterns of proteins and their encoding genes exhibited specific characteristics in the processes of cell division and rhizoid tip growth, which include heterotrophic and autotrophic metabolisms, de novo protein synthesis and active protein turnover, reactive oxygen species and hormone (brassinosteroid and ethylene) signaling, and vesicle trafficking and cytoskeleton dynamic. In addition, the function skew of proteins in fern spores highlights the unique and common mechanisms when compared with evolutionarily divergent spermatophyte pollen. These findings provide an improved understanding of the typical single-celled asymmetric division and polar growth during fern spore germination. Molecular & Cellular

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Section: Heterotrophic and Autotrophic Metabolisms Are Active Inmentioning

confidence: 72%

Section: Heterotrophic and Autotrophic Metabolisms Are Active Inmentioning

confidence: 99%

Cytological and Proteomic Analyses of Osmunda cinnamomea Germinating Spores Reveal Characteristics of Fern Spore Germination and Rhizoid Tip Growth*

Suo

Zhao

Zhang

et al. 2015

Molecular & Cellular Proteomics

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“…33,35,37 These isoenzymes were reported to possess additional non-glycolytic activities correlating with their redox-dependent nuclear or cytosolic localization. Therefore, GapCs possibly function as sensor molecules for the detection of H 2 O 2 diffusing out of the chloroplast or mitochondria upon imbalances of the electron transport chains and are part of a redox-dependent retrograde signal transduction network.…”

Section: -56mentioning

confidence: 99%

“…79,80 Most of these isoforms were also identified during proteome analyses of mature and developing pollen of various plant species. 33,35,37 In addition to the plastid-localized NAD-MDH which is responsible for the removal of NADH to regenerate NAD C to keep the ATP production via plastidial glycolysis running, 2 mitochondrial MDHs were identified. These MDH isoforms possibly contribute to regenerate NADH (in addition to the TCA cycle) which in turn can be used by Complex I to finally synthesize ATP via respiration (Fig.…”

Section: -56mentioning

confidence: 99%

“…23,[27][28][29][30] During the last years, various research groups focused on transcriptome and proteome profiling of different stages of pollen grain maturation and pollen tube growth. [31][32][33][34][35][36][37][38] Approximately half of the identified proteins and transcripts are involved in metabolism, energy generation and cell structure emphasizing the important roles for energy metabolism-related proteins in pollen germination and rapid pollen tube growth. The proteomic data suggest that no alternative oxidase (AOX) protein is present in pollen.…”

Section: Introductionmentioning

confidence: 99%

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Pollen tube growth: where does the energy come from?

Selinski

Scheibe

2014

Plant Signaling & Behavior

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This review focuses on the energy metabolism during pollen maturation and tube growth and updates current knowledge. Pollen tube growth is essential for male reproductive success and extremely fast. Therefore, pollen development and tube growth are high energy-demanding processes. During the last years, various publications (including research papers and reviews) emphasize the importance of mitochondrial respiration and fermentation during male gametogenesis and pollen tube elongation. These pathways obviously contribute to satisfy the high energy demand, and there are many studies which suggest that respiration and fermentation are the only pathways to generate the needed energy. Here, we review data which show for the first time that in addition plastidial glycolysis and the balancing of the ATP/NAD(P)H ratio (by malate valves and NAD C biosynthesis) contribute to satisfy the energy demand during pollen development. Although the importance of energy generation by plastids was discounted during the last years (possibly due to the controversial opinion about their existence in pollen grains and pollen tubes), the available data underline their prime role during pollen maturation and tube growth.

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Cell‐Type‐Specific Proteomics Analysis of a Small Number of Plant Cells by Integrating Laser Capture Microdissection with a Nanodroplet Sample Processing Platform

et al. 2021

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Plant organs and tissues contain multiple cell types, which are well organized in 3-dimensional structure to efficiently perform physiological functions such as homeostasis and response to environmental perturbation and pathogen infection. It is critically important to perform molecular measurements at the cell-type-specific level to discover mechanisms and unique features of cell populations that govern differentiation and respond to external perturbations. Although mass spectrometry−based proteomics has been demonstrated as an enabling discovery tool for studying plant physiology, conventional approaches require millions of cells to generate robust biological conclusions. Such requirements mask the cell-to-cell heterogeneities and limit the comprehensive profiling of plant proteins at spatially resolved and cell-type-specific resolutions. This article describes a recently developed proteomics workflow for studying a small number of plant cells by integrating laser capture microdissection, microfluidic nanodroplet−based sample preparation, and ultrasensitive liquid chromatography−mass spectrometry. Using poplar as a model tree species, we provide detailed protocols, including plant leaf and root tissue harvest, sample preparation, cryosectioning, laser microdissection, protein digestion, mass spectrometry measurement, and data analysis. We show that the workflow enables the precise identification and quantification of thousands of proteins from hundreds of isolated plant root and leaf cells.

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Comparative Proteomic Analysis of Arabidopsis Mature Pollen and Germinated Pollen

Cited by 73 publications

References 65 publications

Cytological and Proteomic Analyses of Osmunda cinnamomea Germinating Spores Reveal Characteristics of Fern Spore Germination and Rhizoid Tip Growth*

Cytological and Proteomic Analyses of Osmunda cinnamomea Germinating Spores Reveal Characteristics of Fern Spore Germination and Rhizoid Tip Growth*

Pollen tube growth: where does the energy come from?

Cell‐Type‐Specific Proteomics Analysis of a Small Number of Plant Cells by Integrating Laser Capture Microdissection with a Nanodroplet Sample Processing Platform

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