Comparative Proteomic Analysis of Mesenchymal Stem Cells Derived from Human Bone Marrow, Umbilical Cord and Placenta: Implication in the Migration

Li, Guo; Zhang, Xiao-Ai; Wang, Hua; Wang, Xin; Meng, Chong; Chan, Cangel Pui-yee; Yew, David T.; Tsang, Kam Sze; Li, Karen; Tsai, Sau-Na; Ngai, Sai-Ming; Han, Zhong Chao; Lin, Marie Chia‐mi; He, Ming; Kung, Hsiang‐Fu

doi:10.1007/978-1-4614-0254-1_5

Cited by 29 publications

(46 citation statements)

References 33 publications

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“…Recently, human placenta-derived MSCs were demonstrated to have higher proliferative potential than ABM-MSCs [19]. Concordant with previous reports, MSCs derived from the three different sources expressed comparable cell surface antigens as well as adipogenic and osteogenic potential [20][21][22]. In addition, they were positive for transcription factor Oct3/4, Sox-2, Nanog and c-Myc.…”

Section: Discussionsupporting

confidence: 73%

Mesenchymal stem cells from bone marrow show a stronger stimulating effect on megakaryocyte progenitor expansion than those from non-hematopoietic tissues

Meng

Yang

Shi³

et al. 2010

Platelets

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In order to evaluate whether mesenchymal stem cells (MSCs) from non-hematopoietic tissues are able to regulate megakaryocytopoiesis, we identified human MSCs from adult bone marrow (ABM), fetal pancreas (FPan) and umbilical cord (UC), and their abilities to support megakaryocyte (MK) differentiation from CD34(+) hematopoietic progenitor cells (HPCs) were comparatively studied. First, MSCs were isolated from ABM, FPan and UC then their growth kinetics, molecular characterization and mesodermal differentiation capacity were determined. ABM-MSCs, FPan-MSCs and UC-MSCs were irradiated and cocultured with human umbilical cord blood (UCB) CD34(+) cells, and the expansion efficiency of MK progenitor cells and MK formation were analysed and compared. Finally, SCF, IL-6 and GM-CSF expression by the three types of MSCs were also examined. Our results showed that FPan-MSCs and UC-MSCs shared most of the characteristic of ABM-MSCs, including morphology, immunophenotype, adipogenic and osteogenic differentiation potentials. Compared with ABM-MSCs, fetal MSCs had higher proliferative capacity. After 7 days' coculture, the maximal production of CD34(+)/CD41a(+) cells was obtained in a group of CD34(+) HPCs + ABM-MSCs. Furthermore, this group produced more MK colonies than other groups (p < 0.05). Surface antigen and ploidy analysis morphological observation demonstrated that a proportion of expanded cells in each group differentiated into mature MKs. ABM-MSCs, FPan-MSCs and UC-MSCs were revealed to express SCF, IL-6 and GM-CSF at mRNA level. We conclude that FPan-MSCs and UC-MSCs have the ability to promote megakaryocytopoiesis, while ABM-MSCs expand more MK progenitor cells from CD34(+) HPCs than MSCs from non-hematopoietic tissues and CD34(+) cells alone.

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Section: Discussionsupporting

confidence: 73%

Mesenchymal stem cells from bone marrow show a stronger stimulating effect on megakaryocyte progenitor expansion than those from non-hematopoietic tissues

Meng

Yang

Shi³

et al. 2010

Platelets

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“…Other molecules including ligands of selectins may also contribute to tissue-specific homing [46]. Furthermore, culture-dependent differences in expression of receptors as well as in migration of MSC [47] have been reported, and the source of cells, e.g., bone marrow, placenta and cord blood, influences migratory potential [48]. In the present study after longer culture periods, MSC showed the same chemotaxis pattern towards apoptotic neurons and cardiomyocytes.…”

Section: Discussionsupporting

confidence: 64%

Hepatocyte growth factor-mediated attraction of mesenchymal stem cells for apoptotic neuronal and cardiomyocytic cells

Vogel

Trapp

Börger

et al. 2009

Cell. Mol. Life Sci.

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Human bone marrow-derived mesenchymal stem cells (MSC) home to injured tissues and have regenerative capacity. In this study, we have investigated in vitro the influence of apoptotic and necrotic cell death, thus distinct types of tissue damage, on MSC migration. Concordant with an increased overall motility, MSC migrated towards apoptotic, but not vital or necrotic neuronal and cardiac cells. Hepatocyte growth factor (HGF) was expressed by the apoptotic cells only. MSC, in contrast, revealed expression of the HGF-receptor, c-Met. Blocking HGF bioactivity resulted in significant reduction of MSC migration. Moreover, recombinant HGF attracted MSC in a dose-dependent manner. Thus, apoptosis initiates chemoattraction of MSC via the HGF/c-Met axis, thereby linking tissue damage to the recruitment of cells with regenerative potential.

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“…Previously, Zhang et al, 2012, demonstrated that exosomes released from UC-MSC are internalized into umbilical cord endothelial cells and enhance in vitro the proliferation and network formation in a dose-dependent manner [37]. Interestingly, pMSC have ∼3.2-fold higher than that UC-MSC migration capacity [20]. Recently, Mineo et al ., reported that the effect of exosomes on angiogenesis involves the Src family of kinases [45].…”

Section: Discussionmentioning

confidence: 99%

“…MSCs are isolated from various sources including bone marrow (principal source), adipose tissue and placenta. Within the human placenta, MSC have been isolated from umbilical cord blood and chorionic villi [19], [20] displaying phenotypes comparable to those isolated from bone marrow, including surface antigen expression (CD45 − , CD14 − , CD19 − , CD80 + , CD86 + , CD40 + and B7H2 + ) and the capacity to differentiate into multiple linages in vitro . MSC have been implicated in wound healing and display the ability to migrate to sites of injury and engage in tissue repair and regeneration of bone, cartilage, liver tissue or myocardial cells [21], [22].…”

Section: Introductionmentioning

confidence: 99%

Exosomal Signaling during Hypoxia Mediates Microvascular Endothelial Cell Migration and Vasculogenesis

et al. 2013

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Vasculogenesis and angiogenesis are critical processes in fetal circulation and placental vasculature development. Placental mesenchymal stem cells (pMSC) are known to release paracrine factors (some of which are contained within exosomes) that promote angiogenesis and cell migration. The aims of this study were: to determine the effects of oxygen tension on the release of exosomes from pMSC; and to establish the effects of pMSC-derived exosomes on the migration and angiogenic tube formation of placental microvascular endothelial cells (hPMEC). pMSC were isolated from placental villi (8–12 weeks of gestation, n = 6) and cultured under an atmosphere of 1%, 3% or 8% O2. Cell-conditioned media were collected and exosomes (exo-pMSC) isolated by differential and buoyant density centrifugation. The dose effect (5–20 µg exosomal protein/ml) of pMSC-derived exosomes on hPMEC migration and tube formation were established using a real-time, live-cell imaging system (Incucyte™). The exosome pellet was resuspended in PBS and protein content was established by mass spectrometry (MS). Protein function and canonical pathways were identified using the PANTHER program and Ingenuity Pathway Analysis, respectively. Exo-pMSC were identified, by electron microscopy, as spherical vesicles, with a typical cup-shape and diameters around of 100 nm and positive for exosome markers: CD63, CD9 and CD81. Under hypoxic conditions (1% and 3% O2) exo-pMSC released increased by 3.3 and 6.7 folds, respectively, when compared to the controls (8% O2; p<0.01). Exo-pMSC increased hPMEC migration by 1.6 fold compared to the control (p<0.05) and increased hPMEC tube formation by 7.2 fold (p<0.05). MS analysis identified 390 different proteins involved in cytoskeleton organization, development, immunomodulatory, and cell-to-cell communication. The data obtained support the hypothesis that pMSC-derived exosomes may contribute to placental vascular adaptation to low oxygen tension under both physiological and pathological conditions.

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Comparative Proteomic Analysis of Mesenchymal Stem Cells Derived from Human Bone Marrow, Umbilical Cord and Placenta: Implication in the Migration

Cited by 29 publications

References 33 publications

Mesenchymal stem cells from bone marrow show a stronger stimulating effect on megakaryocyte progenitor expansion than those from non-hematopoietic tissues

Mesenchymal stem cells from bone marrow show a stronger stimulating effect on megakaryocyte progenitor expansion than those from non-hematopoietic tissues

Hepatocyte growth factor-mediated attraction of mesenchymal stem cells for apoptotic neuronal and cardiomyocytic cells

Exosomal Signaling during Hypoxia Mediates Microvascular Endothelial Cell Migration and Vasculogenesis

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