Comparative proteomic analysis of phenol degradation process by Arthrobacter

Li, Fēi; Song, Wei; Wei, Jiping; Liu, Chongji; Yu, Chunjing

doi:10.1016/j.ibiod.2016.03.023

Cited by 20 publications

(12 citation statements)

References 22 publications

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“…A number of studies report bacteria that are capable of metabolizing phenol, which belong to genera Pseudomonas [ 10 , 12 , 13 ], Acinetobacter [ 2 , 14 ], Rhodococcus [ 3 , 15 ], Bacillus [ 16 ], and Arthrobacter [ 17 , 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Phenol Degradation by Pseudarthrobacter phenanthrenivorans Sphe3

et al. 2023

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Phenol poses a threat as one of the most important industrial environmental pollutants that must be removed before disposal. Biodegradation is a cost-effective and environmentally friendly approach for phenol removal. This work aimed at studying phenol degradation by Pseudarthrobacter phenanthrenivorans Sphe3 cells and also, investigating the pathway used by the bacterium for phenol catabolism. Moreover, alginate-immobilized Sphe3 cells were studied in terms of phenol degradation efficiency compared to free cells. Sphe3 was found to be capable of growing in the presence of phenol as the sole source of carbon and energy, at concentrations up to 1500 mg/L. According to qPCR findings, both pathways of ortho- and meta-cleavage of catechol are active, however, enzymatic assays and intermediate products identification support the predominance of the ortho-metabolic pathway for phenol degradation. Alginate-entrapped Sphe3 cells completely degraded 1000 mg/L phenol after 192 h, even though phenol catabolism proceeds slower in the first 24 h compared to free cells. Immobilized Sphe3 cells retain phenol-degrading capacity even after 30 days of storage and also can be reused for at least five cycles retaining more than 75% of the original phenol-catabolizing capacity.

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Section: Introductionmentioning

confidence: 99%

Phenol Degradation by Pseudarthrobacter phenanthrenivorans Sphe3

et al. 2023

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“…Energy production in the conditions of exposure to toxic substances is usually enhanced, and necessary proteins are upregulated as reported earlier. 19,23 Our study shows that microorganisms use a specific metabolic path to prevent energy consumption and carbon leakage. That is, the key enzyme isocitrate lyase and AceA ( Fig.…”

Section: Resultsmentioning

confidence: 83%

A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-tert-butylphenol and proteomic changes in the degraderPseudomonas aeruginosasan ai

et al. 2019

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The Pseudomonas aeruginosa san ai strain was investigated for its capability to degrade the 2,6-di-tertbutylphenol (2,6-DTBP) plastic additive, a hazardous and toxic substance for aquatic life. This investigation was performed under different parameter values: 2,6-DTBP concentration, inoculum size, pH, and temperature. The GC-MS study showed that P. aeruginosa efficiently degraded 2,6-DTBP in the pH range of 5-8 at higher temperatures. Under exposure to 2,6-DTBP concentrations of 2, 10, and 100 mg L À1 , the strain degraded by 100, 100, and 85%, respectively, for 7 days. Crude enzyme preparation from the biomass of P. aeruginosa san ai showed higher efficiency in 2,6-DTBP removal than that shown by whole microbial cells. Gene encoding for the enzymes involved in the degradation of aromatic compounds in P. aeruginosa san ai was identified. To complement the genomic data, a comparative proteomic study of P. aeruginosa san ai grown on 2,6-DTBP or sunflower oil was conducted by means of nanoLC-MS/MS. The presence of aromatic substances resulted in the upregulation of aromatic ring cleavage enzymes, whose activity was confirmed by enzymatic tests; therefore, it could be concluded that 2,6-DTBP might be degraded by ortho-ring cleavage. A comparative proteomics study of P. aeruginosa san ai indicated that the core molecular responses to aromatic substances can be summarized as the upregulation of proteins responsible for amino acid metabolism with emphasized glutamate metabolism and energy production with upregulated enzymes of glyoxylate bypass. P. aeruginosa san ai has a high capacity to efficiently degrade aromatic compounds, and therefore its whole cells or enzymes could be used in the treatment of contaminated areas.

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“…In this study, we found that protocatechuate 3,4-dioxygenase was down-regulated and 3-carboxy- cis , cis -muconate cycloisomerase, 4-carboxymuconolactone decarboxylase, and 3-oxoadipate enol-lactonase were up-regulated, not only cultured in 2 mM phenol but also in 4 mM phenol compared with control groups. In addition, a previous study found that the protocatechuate branch of the β-ketoadipate pathway was used by Arthrobacter for phenol degradation, as protocatechuate 3,4-dioxygenase, 3-carboxy- cis , cis -muconate cycloisomerase, and 4-carboxymuconolactone decarboxylase were up-regulated during phenol degradation ( Li et al, 2016 ). Similarly, when Pseudomonas putida KT2440 is cultured in medium with benzoate as the sole carbon source, the protocatechuate branch of the β-ketoadipate pathway was found to be activated using the 1-DE MudPIT method ( Yun et al, 2011 ).…”

Section: Resultsmentioning

confidence: 99%

Isolation and Transcriptome Analysis of Phenol-Degrading Bacterium From Carbon–Sand Filters in a Full-Scale Drinking Water Treatment Plant

Zhang

et al. 2018

Front. Microbiol.

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Phenol is a typical organic contaminant in the environment. To date, the biodegradation of phenol by microorganisms remains the preferred method for its removal and remediation, but data on phenol removal by drinking water biofilters are lacking. In this study, we used high-throughput sequencing to investigate the microbial community structure in a carbon–sand biofilter. The results indicated that the predominant bacterial group was Bacilli, followed by Gammaproteobacteria, Clostridia, and Alphaproteobacteria. In addition, a strain was capable of degrading phenol at low concentrations of 500 μg/L within 100 min was isolated and identified as Rhodococcus sp. CS-1. Transcriptome analysis results showed that Rhodococcus sp. CS-1 was able to degrade phenol via both the catechol and protocatechuate branch of the β-ketoadipate pathway. Furthermore, some novel candidate biomarkers (copper oxidase, copper chaperone, and MarR/DeoR/TetR family transcriptional regulators) were successfully identified to be potentially involved in phenol biodegradation. This study indicates that carbon–sand filters have the potential for remediation of phenol. The application of native microorganisms to drinking water treatment system is an adaptive strategy in oligotrophic water environments.

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Comparative proteomic analysis of phenol degradation process by Arthrobacter

Cited by 20 publications

References 22 publications

Phenol Degradation by Pseudarthrobacter phenanthrenivorans Sphe3

Phenol Degradation by Pseudarthrobacter phenanthrenivorans Sphe3

A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-tert-butylphenol and proteomic changes in the degraderPseudomonas aeruginosasan ai

Isolation and Transcriptome Analysis of Phenol-Degrading Bacterium From Carbon–Sand Filters in a Full-Scale Drinking Water Treatment Plant

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