Comparative proteomic analysis of Populus trichocarpa early stem from primary to secondary growth

Liu, Jinwen; Hai, Guanghui; Wang, Chong; Cao, Shenquan; Xü, Wei; Jia, Zhigang; Yang, Chuanping; Wang, Jack; Dai, Shaojun; Cheng, Yuxiang

doi:10.1016/j.jprot.2015.05.032

Cited by 24 publications

(21 citation statements)

References 62 publications

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“…Using this dye, signal from lignified tissue can be detected at 530 nm and non-lignified tissue at 600 nm ( Donaldson et al, 2001 ). We observed similar patterns using the Phloroglucinol, Toluidine blue, UV-autofluorescence, and Acriflavine in detecting lignified tissues (Supplementary Figure 3 ), as observed by others (e.g., Hossain et al, 2012 ; Bonawitz et al, 2014 ; Liu et al, 2015 ). When we observed the emission signal for non-lignified tissue with a CLSM, we found a very interesting pattern in the medial region ( Figure 4 ).…”

Section: Resultssupporting

confidence: 86%

Exploring Cell Wall Composition and Modifications During the Development of the Gynoecium Medial Domain in Arabidopsis

Herrera-Ubaldo

Folter

2018

Front. Plant Sci.

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In Arabidopsis, the gynoecium, the inner whorl of the flower, is the female reproductive part. Many tissues important for fertilization such as the stigma, style, transmitting tract, placenta, ovules, and septum, comprising the medial domain, arise from the carpel margin meristem. During gynoecium development, septum fusion occurs and tissues form continuously to prepare for a successful pollination and fertilization. During gynoecium development, cell wall modifications take place and one of the most important is the formation of the transmitting tract, having a great impact on reproductive competence because it facilitates pollen tube growth and movement through the ovary. In this study, using a combination of classical staining methods, fluorescent dyes, and indirect immunolocalization, we analyzed cell wall composition and modifications accompanying medial domain formation during gynoecium development. We detected coordinated changes in polysaccharide distribution through time, cell wall modifications preceding the formation of the transmitting tract, mucosubstances increase during transmitting tract formation, and a decrease of mannan distribution. Furthermore, we also detected changes in lipid distribution during septum fusion. Proper cell wall composition and modifications are important for postgenital fusion of the carpel (septum fusion) and transmitting tract formation, because these tissues affect plant reproductive competence.

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Section: Resultssupporting

confidence: 86%

Exploring Cell Wall Composition and Modifications During the Development of the Gynoecium Medial Domain in Arabidopsis

Herrera-Ubaldo

Folter

2018

Front. Plant Sci.

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“…A previous proteomic study on Populus secondary cell wall formation provided clues for the existence of this novel function. The abundance of PtrEPSP-TF, but not PtrEPSP-SY or any other enzymes involved in the shikimate pathway, was found to increase during primary to secondary growth of Populus stem development (Liu et al, 2015). To support this observation, we found that the expression of PtrEPSP-TF was highest in the relatively chloroplast-devoid developing xylem tissue, where Pt-EPSP-SY exhibited extremely low expression in the same tissue (Supplemental Figure 6).…”

Section: Discussionmentioning

confidence: 66%

A 5-Enolpyruvylshikimate 3-Phosphate Synthase Functions as a Transcriptional Repressor in Populus

et al. 2018

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Long-lived perennial plants, with distinctive habits of inter-annual growth, defense, and physiology, are of great economic and ecological importance. However, some biological mechanisms resulting from genome duplication and functional divergence of genes in these systems remain poorly studied. Here, we discovered an association between a poplar ( 5-enolpyruvylshikimate 3-phosphate synthase gene () and lignin biosynthesis. Functional characterization of PtrEPSP revealed that this isoform possesses a helix-turn-helix motif in the N terminus and can function as a transcriptional repressor that regulates expression of genes in the phenylpropanoid pathway in addition to performing its canonical biosynthesis function in the shikimate pathway. We demonstrated that this isoform can localize in the nucleus and specifically binds to the promoter and represses the expression of a -like transcriptional regulator, which itself specifically binds to the promoter and represses the expression of (known as in), a master regulator of the phenylpropanoid pathway and lignin biosynthesis. Analyses of overexpression and RNAi lines targeting PtrEPSP confirmed the predicted changes in expression patterns. These results demonstrate that PtrEPSP in its regulatory form and PtrhAT form a transcriptional hierarchy regulating phenylpropanoid pathway and lignin biosynthesis in.

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“…The transcription of PtrEPSP-TF is under the control of the master regulator PtrWND1B, a homolog of AtSND1. Notably, the abundance of PtrEPSP-TF increases during the transition from primary to secondary growth in the stem [64], thus providing an elegant regulatory mechanism for the monolignol biosynthetic pathway.…”

Section: R2r3 Mybs the Gatekeepers Of Scw Formation And Lignificationmentioning

confidence: 99%

A Molecular Blueprint of Lignin Repression

Behr¹,

Guerriero²,

Grima‐Pettenati³

et al. 2019

Trends in Plant Science

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Although lignin is essential to ensure the correct growth and development of land plants, it may be an obstacle to the production of lignocellulosics-based biofuels, and reduces the nutritional quality of crops used for human consumption or livestock feed. The need to tailor the lignocellulosic biomass for more efficient biofuel production or for improved plant digestibility has fostered considerable advances in our understanding of the lignin biosynthetic pathway and its regulation. Most of the described regulators are transcriptional activators of lignin biosynthesis, but considerably less attention has been devoted to the repressors of this pathway. We provide a comprehensive overview of the molecular factors that negatively impact on the lignification process at both the transcriptional and post-transcriptional levels. Challenging LigninLignin is a major cell wall component that fulfills fundamental functions in plant development as well as in defense against pests and pathogens. This heteropolymer impregnates the compound middle lamella and the secondary cell walls (SCWs) of cells genetically programmed to be lignified, such as xylem tracheary elements as well as xylem and phloem fibers. Lignin biosynthesis spans the phenylpropanoid and the monolignol pathways, from phenylalanine to p-coumaryl, coniferyl, and sinapyl alcohols. Monolignol homeostasis is regulated through a balance between the molecular factors that antagonistically regulate their biosynthesis. After monolignols are excreted into the apoplast, they are oxidized by the phenol-oxidoreductase laccases and/or by class III peroxidases, and are polymerized by radical coupling. A complex regulatory network involving phytohormones, transcription factors (TFs), and post-transcriptional events guide SCW deposition and lignification [1]. This network prevents lignin deposition in tissues undergoing active division or elongation such as apical meristems, vascular cambium, and immature xylem cells, as well as in non-lignified tissues such as stem pith and flax or hemp bast fibers that harbor gelatinous walls under normal developmental conditions. Similarly, this network negatively regulates lignification in xylem tissue formed in response to mechanical stresses such as, for instance, poplar tension wood that harbors hypolignified gelatinous walls.

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Comparative proteomic analysis of Populus trichocarpa early stem from primary to secondary growth

Cited by 24 publications

References 62 publications

Exploring Cell Wall Composition and Modifications During the Development of the Gynoecium Medial Domain in Arabidopsis

Exploring Cell Wall Composition and Modifications During the Development of the Gynoecium Medial Domain in Arabidopsis

A 5-Enolpyruvylshikimate 3-Phosphate Synthase Functions as a Transcriptional Repressor in Populus

A Molecular Blueprint of Lignin Repression

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