Comparative Proteomic Analysis of Propane Metabolism in &lt;b&gt;&lt;i&gt;Mycobacterium&lt;/i&gt;&lt;/b&gt; sp. Strain ENV421 and &lt;b&gt;&lt;i&gt;Rhodococcus&lt;/i&gt;&lt;/b&gt; sp. Strain ENV425

Tupa, Peter R.; Masuda, Hisako

doi:10.1159/000490494

Cited by 12 publications

(4 citation statements)

References 44 publications

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“…ENV421 and Rhodococcus rhodochrous 21198 , (Table ). Further, the presence of propane can also upregulate the polycistronic transcription of the prmABCD clusters in PH-06 and ENV421, , which subsequently promoted the activity of dioxane biotransformation. Our study revealed that this single PRM enzyme can degrade both dioxane and gaseous alkanes.…”

Section: Resultsmentioning

confidence: 99%

Distinct Catalytic Behaviors between Two 1,4-Dioxane-Degrading Monooxygenases: Kinetics, Inhibition, and Substrate Range

Deng

2019

Environ. Sci. Technol.

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Monitored natural attenuation (MNA) and engineered bioremediation have been recognized as effective and cost-efficient in situ treatments to mitigate 1,4-dioxane (dioxane) contamination. Dioxane metabolism can be initiated by two catabolic enzymes, propane monooxygenase (PRM) and tetrahydrofuran monooxygenase (THM), belonging to the group-6 and 5 of soluble di-iron monooxygenase family, respectively. In this study, we comprehensively compared catalytic behaviors of PRM and THM when individually expressed in the heterologous host, Mycobacterium smegmatis mc 2 -155. Kinetic results revealed a half-saturation coefficient (K m ) of 53.0 ± 13.1 mg/L for PRM, nearly 4 times lower than that of THM (235.8 ± 61.6 mg/L), suggesting that PRM has a higher affinity to dioxane. Exposure with three common co-contaminants (1,1dichloroethene, trichloroethene, and 1,1,1-trichloroethane) demonstrated that PRM was also more resistant to their inhibition than THM. Thus, dioxane degraders expressing PRM may be more physiologically and ecologically advantageous than those with THM at impacted sites, where dioxane concentration is relatively low (e.g., 250 to 1000 μg/L) with co-occurrence of chlorinated solvents (e.g., 0.5 to 8 mg/L), underscoring the need of surveying both PRM and THM-encoding genes for MNA potential assessment. PRM is also highly versatile, which breaks down cyclic molecules (dioxane, tetrahydrofuran, and cyclohexane), as well as chlorinated and aromatic pollutants, including vinyl chloride, 1,2-dichloroethane, benzene, and toluene. This is the first report regarding the ability of PRM to degrade a variety of short-chain alkanes and ethene in addition to dioxane, unraveling its pivotal role in aerobic biostimulation that utilizes propane, isobutane, or other gaseous alkanes/alkenes (e.g., ethane, butane, and ethene) to select and fuel indigenous microorganisms to tackle the commingled contamination of dioxane and chlorinated compounds.

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Section: Resultsmentioning

confidence: 99%

Distinct Catalytic Behaviors between Two 1,4-Dioxane-Degrading Monooxygenases: Kinetics, Inhibition, and Substrate Range

Deng

2019

Environ. Sci. Technol.

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“…All the previous proteomic investigations, which were performed on Rhodococcus , only highlighted its catabolic behavior towards different organic compounds viz . Fluoranthene 14 , toluene and phenol 15 , propane 16 , triacylglycerol 17 , 4,4′-Dithiodibutyric Acid 18 , benzoate 19 and polyaromatic compounds 20 . In this perspective, the present study for the first time unravels its proteomic response towards the abiotic stress and identified a protein biomarker for low-temperature nitrogen deficient conditions.…”

Section: Discussionmentioning

confidence: 99%

Differential protein profiling of soil diazotroph Rhodococcus qingshengii S10107 towards low-temperature and nitrogen deficiency

Suyal

Juyal

Kumar

et al. 2019

Sci Rep

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Protein-based biomarkers can be a promising approach for identification and real-time monitoring of the bio-inoculants employed under sustainable agricultural plans. In this perspective, differential proteomics of psychrophilic diazotroph Rhodococcus qingshengii S10107 (JX173283) was performed to unravel its adaptive responses towards low-temperature nitrogen deficiency and identification of a biomarker for respective physiological conditions. LC-MS/MS-based proteome analysis mapped more than 4830 proteins including 77 up-regulated and 47 down-regulated proteins (p ≤ 0.05). Differential expression of the structural genes of nif regulon viz. nifH, nifD, and nifK along with their response regulators i.e. nifA, nifL, and nifB indicated that the nitrogenase complex was activated successfully. Besides up-regulating the biosynthesis of certain amino acids viz. Leucine, Lysine, and Alanine; the expression of the peptidoglycan synthesis proteins were also increased; while, the enzymes involved in Lipid biosynthesis were found to decrease. Furthermore, two important enzymes of the pentose phosphate pathway viz. Transketolase and Transaldolase along with Ribose import ATP-binding protein RbsA were also found to induce significantly under low temperature a nitrogen deficient condition, which suggests the cellular need for ample ribose sugar instantly. Additionally, comparative protein profiling of S10107 strain with our previous studies revealed that CowN protein was significantly up-regulated in all the cases under low-temperature nitrogen deficient conditions and therefore, can be developed as a biomarker. Conclusively, present study for the first time provides an in-depth proteome profiling of R. qingshengii S10107 and proclaims CowN as a potential protein biomarker for monitoring BNF under cold niches.

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“…A similar a MTBE value was obtained for P. mendocina KR-1 (0.28 L g protein À1 h À1 ), which also has a relatively high K s values. 14 Though higher a MTBE values were calculated for Rhodococcus rubber ENV425 (20.76 L g protein À1 h À1 ) 39 and Arthrobacter sp. ATCC 27778 (16.95 L g protein À1 h À1 ), 38,40 TBA accumulation accompanied the MTBE degradation process of lower rate in these systems.…”

Section: Kinetic Characteristics Of Mtbe Co-metabolism By Acinetobactmentioning

confidence: 94%

Characterization of co-metabolic biodegradation of methyl tert-butyl ether by a Acinetobacter sp. strain

Wang

et al. 2019

RSC Adv.

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Co-metabolic bioremediation is a promising approach for the elimination of methyl tert-butyl ether (MTBE), which is a common pollutant found worldwide in ground water. In this paper, a bacterial strain able to cometabolically degrade MTBE was isolated and named as Acinetobacter sp. SL3 based on 16S rRNA gene sequencing analysis. Strain SL3 could grow on n-alkanes (C 5 -C 8 ) accompanied with the co-metabolic degradation of MTBE. The number of carbons present in the n-alkane substrate significantly influenced the degradation rate of MTBE and accumulation of tert-butyl alcohol (TBA), with n-octane resulting in a higher MTBE degradation rate (V max ¼ 36.7 nmol min À1 mg protein À1 , K s ¼ 6.4 mmol L À1 ) and lower TBA accumulation rate. A degradation experiment in a fed-batch reactor revealed that the efficiency of MTBE degradation by Acinetobacter sp. strain SL3 did not show an obvious decrease after nine rounds of MTBE replenishment ranging from 0.1-0.5 mmol L À1 . The results of this paper reveal the preferable properties of Acinetobacter sp. SL3 for the bioremediation of MTBE via co-metabolism and leads towards the development of new MTBE elimination technologies.

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Comparative Proteomic Analysis of Propane Metabolism in <b><i>Mycobacterium</i></b> sp. Strain ENV421 and <b><i>Rhodococcus</i></b> sp. Strain ENV425

Cited by 12 publications

References 44 publications

Distinct Catalytic Behaviors between Two 1,4-Dioxane-Degrading Monooxygenases: Kinetics, Inhibition, and Substrate Range

Distinct Catalytic Behaviors between Two 1,4-Dioxane-Degrading Monooxygenases: Kinetics, Inhibition, and Substrate Range

Differential protein profiling of soil diazotroph Rhodococcus qingshengii S10107 towards low-temperature and nitrogen deficiency

Characterization of co-metabolic biodegradation of methyl tert-butyl ether by a Acinetobacter sp. strain

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