Comparative proteomic analysis of surface proteins of Trichinella spiralis muscle larvae and intestinal infective larvae

Liu, Ruo Dan; Cui, Jing; Liu, Xiao Lin; Jiang, Peng; Sun, Ge; Zhang, Xi; Long, Stuart A.; Wang, Li; Wang, Zhong Quan

doi:10.1016/j.actatropica.2015.07.002

Cited by 96 publications

(91 citation statements)

References 30 publications

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“…The MS/MS data were searched against the T. spiralis protein database from UniProt and NCBI using the SEQUEST algorithm. The SEQUEST search parameters used in this study were Delta CN (≥0.1) and Xcorr (one charge ≥ 1.9, two charges ≥ 2.2, and three charges ≥ 3.75) (Liu et al, 2015). …”

Section: Methodsmentioning

confidence: 99%

Proteomic Analysis of Trichinella spiralis Adult Worm Excretory-Secretory Proteins Recognized by Sera of Patients with Early Trichinellosis

et al. 2017

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The most commonly used serodiagnostic antigens for trichinellosis are the excretory-secretory (ES) antigens from T. spiralis muscle larvae (ML), but the specific antibodies against the ML ES antigens are usually negative during early stage of Trichinella infection. The recent studies demonstrated that T. spiralis adult worm (AW) antigens were recognized by mouse or swine infection sera on Western blot as early as 7–15 days post-infection (dpi), the AW antigens might contain the early diagnostic markers for trichinellosis. The purpose of this study was to screen early diagnostic antigens in T. spiralis AW ES proteins recognized by sera of early patients with trichinellosis. T. spiralis AW were collected at 72 h post-infection (hpi), and their ES antigens were analyzed by SDS-PAGE and Western blot. Our results showed that 5 protein bands (55, 48–50, 45, 44, and 36 kDa) were recognized by sera of early patients with trichinellosis collected at 19 dpi, and were subjected to shotgun LC–MS/MS and bioinformatics analyses. A total of 185 proteins were identified from T. spiralis protein database, of which 116 (67.2%) proteins had molecular weights of 30∼60 kDa, and 125 (67.6%) proteins with pI 4–7. Bioinformatic analyses showed that the identified proteins have a wide diversity of biological functions (binding of nucleotides, proteins, ions, carbohydrates, and lipids; hydrolase, transferase, and oxidoreductase, etc.). Several enzymes (e.g., adult-specific DNase II, serine protease and serine protease inhibitor) could be the invasion-related proteins and early diagnostic markers for trichinellosis. Moreover, recombinant T. spiralis serine protease (rTsSP-ZH68) was expressed in E. coli and its antigenicity was analyzed by Western blot with the early infection sera. The rTsSP-ZH68 was recognized by sera of infected mice at 8–10 dpi and sera of early patients with trichinellosis at 19 dpi. T. spiralis AW proteins identified in this study, especially serine protease, are the promising early diagnostic antigens and vaccine candidates for trichinellosis.

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Section: Methodsmentioning

confidence: 99%

Proteomic Analysis of Trichinella spiralis Adult Worm Excretory-Secretory Proteins Recognized by Sera of Patients with Early Trichinellosis

et al. 2017

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“…New antigens expressed across developmental stages, such as Ts 14-3-3, could be important for diagnosing trichinellosis in the context of co-infection with other parasitic nematodes, since co-infection often increases the incidence of false positives in tests [29]. Other somatic proteins, many of which are expressed on the parasite surface in muscle and intestinal forms of the parasite, may be possible vaccination targets [30]. As might be expected, expression of genes encoding energy-generating proteins is up-regulated in intestinal infective larvae in comparison to muscle larvae [30].…”

Section: Clade Imentioning

confidence: 99%

“…Other somatic proteins, many of which are expressed on the parasite surface in muscle and intestinal forms of the parasite, may be possible vaccination targets [30]. As might be expected, expression of genes encoding energy-generating proteins is up-regulated in intestinal infective larvae in comparison to muscle larvae [30]. …”

Section: Clade Imentioning

confidence: 99%

Perusal of parasitic nematode ‘omics in the post-genomic era

Stoltzfus

Pilgrim

Herbert

2017

Molecular and Biochemical Parasitology

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The advent of high-throughput, next-generation sequencing methods combined with advances in computational biology and bioinformatics have greatly accelerated discovery within biomedical research. This “post-genomics” era has ushered in powerful approaches allowing one to quantify RNA transcript and protein abundance for every gene in the genome - often for multiple conditions. Herein, we chronicle how the post-genomics era has advanced our overall understanding of parasitic nematodes through transcriptomics and proteomics and highlight some of the important advances made in each major nematode clade. We primarily focus on organisms relevant to human health, given that nematode infections significantly impact disability-adjusted life years (DALY) scores within the developing world, but we also discuss organisms of veterinary importance as well as those used as laboratory models. As such, we envision that this review will serve as a comprehensive resource for those seeking a better understanding of basic parasitic nematode biology as well as those interested in targets for vaccination and pharmacological intervention.

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“…Importantly, it was shown that ML cannot invade the intestinal epithelial cells in vitro cultured unless they are exposed to the intestinal milieu or bile and activated into the intestinal infective larvae (IIL) [39][40][41]. The identification of IIL molecules provides attractive information not only to elucidate the mechanism of parasite invasion and immune evasion but also to spiralis IIL displayed on the surface of T7 phage was injected i.p.…”

Section: Second-generation Vaccinesmentioning

confidence: 99%

“…Other proteins with significant higher expression in IIL than in ML such as a putative copper/ zinc superoxide dismutase (SODC), adult-specific DNase II, putative low-density lipoprotein receptor domain class A (LDLRA), and secreting receptor (SR) have been identified [41]. More recently, some important proteins were identified in E/S from IIL, such as the gp53 kDa with serine protease activity, multi-cystatin-like domain and cystatin-like protein, deoxyribonuclease II family protein, among others [42].…”

Section: Second-generation Vaccinesmentioning

confidence: 99%

Vaccination against Trichinella spiralis: Potential, Limitations and Future Directions

Andrade-Becerra¹,

Pompa-Mera²,

MaríaRibas-Aparicio³

et al. 2017

Natural Remedies in the Fight Against Parasites

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Trichinellosis is a food-borne parasitic disease caused by round worms of the genus Trichinella. The majority of human outbreaks are attributed to consumption of raw or undercooked pork meat contaminated with T. spiralis muscle larvae. A blockingtransmission vaccine against trichinellosis will allow preventing swine infection and will contribute to disease control. In this chapter, different vaccine candidates so far developed against T. spiralis, including first-, second-, and third-generation vaccines, are discussed. Most vaccine candidates are based on a unique antigen mainly from the muscle larva stage, inducing with some exceptions, partial protection although a mix Th1/Th2 immune response is elicited. Therefore, the need for identification of new antigens from different parasite stages focusing on infective intestinal larvae, adult, and newborn larvae stages as well as the evaluation of their protective capacity in pigs is presented. The design of multi-epitope vaccines and the use of adjuvants or immunomodulatory molecules capable to polarize the immune response to a Th2-type-protective response are discussed as imperative elements of modern vaccines. Plant-based vaccines and probiotics as excellent tools for vaccine development against T. spiralis are also presented as an attractive platform for veterinary vaccines.

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Comparative proteomic analysis of surface proteins of Trichinella spiralis muscle larvae and intestinal infective larvae

Cited by 96 publications

References 30 publications

Proteomic Analysis of Trichinella spiralis Adult Worm Excretory-Secretory Proteins Recognized by Sera of Patients with Early Trichinellosis

Proteomic Analysis of Trichinella spiralis Adult Worm Excretory-Secretory Proteins Recognized by Sera of Patients with Early Trichinellosis

Perusal of parasitic nematode ‘omics in the post-genomic era

Vaccination against Trichinella spiralis: Potential, Limitations and Future Directions

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