Comparative proteomic analysis of Taurine, Indicine, and crossbred (Bos taurus × Bos indicus) bull spermatozoa for identification of proteins related to sperm malfunctions and subfertility in crossbred bulls

Aslam, Munchakkal Kather Muhammad; Kumaresan, A.; Rajak, S. K.; Tajmul, Md-D.; Datta, Tirtha Kumar; Mohanty, T.K.; Srinivasan, Alagiri; Yadav, Savita

doi:10.1016/j.theriogenology.2015.04.020

Cited by 25 publications

(14 citation statements)

References 42 publications

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“…This indicates the possibilities of compromised capacitation and acrosome reaction in crossbred bulls. Consistent with our earlier results, pre-mature capacitation is a significant problem in crossbred bulls [ 14 ]. However, 38 genes involved in calcium ion binding were also upregulated.…”

Section: Discussionsupporting

confidence: 93%

“…Though axiomatic evidences of inferior reproductive performances in crossbred bulls than Zebu bulls has been demonstrated in terms of genetic [ 10 ], hormonal [ 12 ], semenological [ 7 ] and andrological aspects [ 13 ], the etiology has not been well understood. Our group has been working towards the goal of unravelling the reasons for infertility in crossbred bulls, and found the differences in terms of proportion of sertoli cells in relation to spermatogenic cells [ 13 ], proteomic profile of spermatozoa [ 14 ], seminal plasma [ 15 ], and spermatogenic and sertoli cells [ 16 ]. A majority of these studies indicate altered sperm quality and functions, as the major factor contributing to high incidence of infertility in crossbred bulls.…”

Section: Introductionmentioning

confidence: 99%

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Sub-fertility in crossbred bulls: deciphering testicular level transcriptomic alterations between zebu (Bos indicus) and crossbred (Bos taurus x Bos indicus) bulls

et al. 2020

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Background The incidence of poor semen quality and sub-fertility/infertility is higher in crossbred as compared to Zebu males. Several attempts have been made to understand the possible reasons for higher incidence of fertility problems in crossbred males, at sperm phenotype, proteome and genome level but with variable results. Since the quality of the ejaculated spermatozoa is determined by the testicular environment, assessing the testicular transcriptome between these breeds would help in identifying the possible mechanisms associated with infertility in crossbred bulls. However, such information is not available. We performed global transcriptomic profiling of testicular tissue from crossbred and Zebu bulls using Agilent Bos taurus GXP 8X60k AMADID: 29411 array. To the best of our knowledge, this is the first study comparing the testicular mRNAs between crossbred and Zebu bulls. Results Out of the 14,419 transcripts detected in bovine testis, 1466 were differentially expressed between crossbred and Zebu bulls, in which 1038 were upregulated and 428 were downregulated in crossbred bulls. PI4KB and DPY19L2 genes, reported to be involved in sperm capacitation and acrosome formation respectively, were among the top 10 downregulated transcripts in crossbred testis. Genes involved in ubiquitination and proteolysis were upregulated, while genes involved in cell proliferation, stem cell differentiation, stem cell population maintenance, steroidogenesis, WNT signalling, protein localization to plasma membrane, endocannabinoid signalling, heparin binding, cAMP metabolism and GABA receptor activity were downregulated in crossbred testis. Among the 10 genes validated using qPCR, expression of CCNYL, SOX2, MSMB, SPATA7, TNP1, TNP2 and CRISP2 followed the same trend as observed in microarray analysis with SPATA7 being significantly downregulated and transition proteins ( TNP1 , TNP2 ) being significantly upregulated in crossbred bulls. Conclusions Abundant proteolysis by ubiquitination and downregulation of WNT signaling, cell proliferation, differentiation and steroidogenesis might be associated with higher incidence of poor semen quality and/or sub-fertility/infertility in crossbred bulls as compared to Zebu bulls. Downregulation of SPATA7 (Spermatogenesis Associated 7) and upregulation of transition proteins ( TNP1 and TNP2 ) in crossbred bull testis might be associated with impaired spermatogenesis processes including improper chromatin compaction in crossbred bulls.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Sub-fertility in crossbred bulls: deciphering testicular level transcriptomic alterations between zebu (Bos indicus) and crossbred (Bos taurus x Bos indicus) bulls

et al. 2020

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“…The recent developments in the field of omics technologies and its widespread use in reproduction studies provide ample opportunities to address such issues with commendable outcome. For the past few years, our laboratory is involved in developing reliable proteomic biomarkers for the early determination fertility in dairy bulls (Aslam et al, 2015(Aslam et al, , 2018Tripathi et al, 2014). In this report, the differentially ex- (Baba et al, 1994;Polakoski & Parrish, 1977;Whitehurst et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…From each bull, cryopreserved semen straws (produced from three different ejaculates and freezing operations) were collected, thawed at 37°C for 30 s and spermatozoa were separated by centrifugation (1,000 g for 15 min at 4°C) after adding 1% (v:v) protease inhibitor cocktail (Amresco, USA). Equal quantities of spermatozoa from different ejaculates of individual bulls were pooled, and protein samples were prepared as reported in our previous reports (Aslam et al, 2015(Aslam et al, , 2018. The protein concentrations were estimated by Bradford method and stored at −80°C till further use.…”

Section: Experimental Bulls and Fertility Classificationmentioning

confidence: 99%

“…Several researchers reported that the fertilization ability of spermatozoa is invariably related to alterations in expression levels of specific proteins (Aslam et al, , 2018D'Amours, Frenette, Fortier, Leclerc, & Sullivan, 2010;Peddinti et al, 2008). Earlier, using the proteomic approach, we identified some potential fertility-associated proteins in the semen of crossbred bulls (Aslam et al, 2015(Aslam et al, , 2018. Despite increasing research on sperm proteomics, identification of fertility-associated proteins in spermatozoa remains elusive in case of buffalo bulls.…”

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Comparative proteomic analysis of high‐ and low‐fertile buffalo bull spermatozoa for identification of fertility‐associated proteins

Aslam

Kumaresan

Yadav

et al. 2019

Reprod Domestic Animals

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Contents The present study identified few potential proteins in the spermatozoa of buffalo bulls that can be used as an aid in fertility determination through comparative proteomics. The sperm proteome of high‐fertile buffalo bulls was compared with that of low‐fertile buffalo bulls using two‐dimensional difference gel electrophoresis (2D‐DIGE), and the differentially expressed proteins were identified through mass spectrometric method. The protein interaction network and the functional bioinformatics analysis of differentially expressed proteins were also carried out. In the spermatozoa of high‐fertile bulls, 10 proteins were found overexpressed and 15 proteins were underexpressed at the level of twofold or more (p ≤ 0.05). The proteins overexpressed in high‐fertile spermatozoa were PDZD8, GTF2F2, ZNF397, KIZ, LOH12CR1, ACRBP, PRSS37, CYP11B2, F13A1 and SPO11, whereas those overexpressed in low‐fertile spermatozoa were MT1A, ATP5F1, CS, TCRB, PRODH2, HARS, IDH3A, SRPK3, Uncharacterized protein C9orf9 homolog isoform X4, TUBB2B, GPR4, PMP2, CTSL1, TPPP2 and EGFL6. The differential expression ranged from 2.0‐ to 6.1‐fold between the two groups, where CYP11B2 was high abundant in high‐fertile spermatozoa and MT1A was highly abundant in low‐fertile spermatozoa. Most of the proteins overexpressed in low‐fertile spermatozoa were related to energy metabolism and capacitation factors, pointing out the possible role of pre‐mature capacitation and cryo‐damages in reducing the fertility of cryopreserved buffalo spermatozoa.

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Metabolomic fingerprinting of bull spermatozoa for identification of fertility signature metabolites

Saraf

Kumaresan

Dasgupta

et al. 2020

Molecular Reproduction Devel

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The objective of the study was to identify the fertility‐associated metabolites in bovine spermatozoa using liquid chromatography‐mass spectrometry (LC‐MS). Six Holstein Friesian crossbred bulls (three high‐fertile and three low‐fertile bulls) were the experimental animals. Sperm proteins were isolated and protein‐normalized samples were processed for metabolite extraction and subjected to LC‐MS/MS analysis. Mass spectrometry data were processed using iMETQ software and metabolites were identified using Human Metabolome DataBase while, Metaboanalyst 4.0 tool was used for statistical and pathway analysis. A total of 3,704 metabolites belonging to various chemical classes were identified in bull spermatozoa. After sorting out exogenous metabolites, 56 metabolites were observed common to both the groups while 44 and 35 metabolites were found unique to high‐ and low‐fertile spermatozoa, respectively. Among the common metabolites, concentrations of 19 metabolites were higher in high‐fertile compared to low‐fertile spermatozoa (fold change > 1.00). Spermatozoa metabolites with variable importance in projections score of more than 1.5 included hypotaurine, d‐cysteine, selenocystine. In addition, metabolites such as spermine and l‐cysteine were identified exclusively in high‐fertile spermatozoa. Collectively, the present study established the metabolic profile of bovine spermatozoa and identified the metabolomic differences between spermatozoa from high‐ and low‐fertile bulls. Among the sperm metabolites, hypotaurine, selenocysteine, l‐malic acid, d‐cysteine, and chondroitin 4‐sulfate hold the potential to be recognized as fertility‐associated metabolites.

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Comparative proteomic analysis of Taurine, Indicine, and crossbred (Bos taurus × Bos indicus) bull spermatozoa for identification of proteins related to sperm malfunctions and subfertility in crossbred bulls

Cited by 25 publications

References 42 publications

Sub-fertility in crossbred bulls: deciphering testicular level transcriptomic alterations between zebu (Bos indicus) and crossbred (Bos taurus x Bos indicus) bulls

Sub-fertility in crossbred bulls: deciphering testicular level transcriptomic alterations between zebu (Bos indicus) and crossbred (Bos taurus x Bos indicus) bulls

Comparative proteomic analysis of high‐ and low‐fertile buffalo bull spermatozoa for identification of fertility‐associated proteins

Metabolomic fingerprinting of bull spermatozoa for identification of fertility signature metabolites

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