Comparative Proteomic Analysis of the Stolon Cold Stress Response between the C4 Perennial Grass Species Zoysia japonica and Zoysia metrella

Xuan, Jinsong; Song, Yong; Zhang, Hongxiao; Liu, Jianxiu; Guo, Zhenjiang; Hua, Yuelou

doi:10.1371/journal.pone.0075705

Cited by 36 publications

(26 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Surprisingly, we found that cold stress induced an increase in the expression of SOD and APX in banana leaves, while the expression of another isoenzyme of SOD slightly decreased ( Table 1). The simultaneous up-and down-regulation in different isoenzymes has been detected under cold stress conditions, similar to what has been described in zoysiagrass (Zoysia japonica) stolons exposed to cold stress, and Xuan et al (2013) confirmed that functionally different APX proteins differentially respond to cold stress. Scavenging for increased ROS at low temperatures can be achieved by shifting protein expression to produce isoforms that have improved performance at low temperature.…”

Section: Discussionsupporting

confidence: 82%

“…RuBisCo activase and MCPR were found to be up-regulated in banana leaves under cold stress in this study, but the alpha subunit of Rlsbp was decreased (Table 1). Cold treatment can increase the abundance of RuBisCo and RuBisCo activase (Dumont et al, 2011;Wang et al, 2009;Xuan et al, 2013). When photosynthesis is greatly inhibited by low temperature in various crops (Allen and Ort, 2001;Jin et al, 2011), they can increase the levels of RuBisCo, RuBisCo activase, and MCPR, which are needed to compensate for decreased activities of the enzymes at low temperatures (Yamori et al, 2014).…”

Section: Involvement In Photosynthesismentioning

confidence: 99%

See 1 more Smart Citation

Proteomic Analysis of Cold Stress Responses in Banana Leaves

Ren¹,

Zhang²,

Wang³

et al. 2015

J. Amer. Soc. Hort. Sci.

View full text Add to dashboard Cite

Cold stress is one of the most important environmental factors affecting crop growth and agricultural production. Induced changes of gene expression and metabolism are critical for plants responding and acclimating to cold stress. Banana (Musa sp.) is one of the most important food crops in the tropical and subtropical countries of the world. Banana, which originated from tropical regions, is sensitive to cold, which can result in serious losses in commercial banana production. To investigate the response of the banana to cold stress conditions, changes in protein expression were analyzed using a comparative proteomics approach. ‘Brazil’ banana (Musa acuminata AAA group) is a common banana cultivar in southern China. ‘Brazil’ banana plantlets were exposed to 5 °C for 24 hours and then total crude protein was extracted from treatment and control leaves by phenol extraction, separated with two-dimensional gel electrophoresis, and subsequently identified by mass spectrometry (MS). Out of the more than 400 protein spots reproducibly detected, only 41 protein spots exhibited a change in intensity by at least 2-fold, with 26 proteins increasing and 15 proteins decreasing expression. Of these, 28 differentially expressed proteins were identified by MS. The identified proteins, including well-known and novel cold-responsive proteins, are involved in several cellular processes, including antioxidation and antipathogen, photosynthesis, chaperones, protein synthesis, signal transduction, energy metabolism, and other cellular functions. Proteins related to antioxidation, pathogen resistance, molecular chaperones, and energy metabolism were up-regulated, and proteins related to ethylene synthesis, protein synthesis, and epigenetic modification were down-regulated in response to cold temperature treatment. The banana plantlets incubated at cold temperatures demonstrated major changes in increased reactive oxygen species (ROS) scavenging, defense against diseases, and energy supply. Increased antioxidation capability in banana was also discovered in plantain, which has greater cold tolerance than banana in response to cold stress conditions. Therefore, we hypothesized that an increased antioxidation ability could be a common characteristic of banana and plantain in response to cold stress conditions. These findings may provide a better understanding of the physiological processes of banana in response to cold stress conditions.

show abstract

Section: Discussionsupporting

confidence: 82%

Section: Involvement In Photosynthesismentioning

confidence: 99%

Proteomic Analysis of Cold Stress Responses in Banana Leaves

Ren¹,

Zhang²,

Wang³

et al. 2015

J. Amer. Soc. Hort. Sci.

View full text Add to dashboard Cite

show abstract

“…Ca 2+ acts as a secondary messenger in salt stress responses (Xuan et al, 2013 ). The cytosolic free Ca 2+ concentration ([Ca 2+ ] cyt ) changes within seconds in plants subjected to salt stress (Knight et al, 1997 ), leading to downstream signaling.…”

Section: Resultsmentioning

confidence: 99%

“…Z. japonica has a similar ROS scavenging system to that of other plant species. Cold treatment significantly increases antioxidant enzyme levels (Xuan et al, 2013 ), which may provide protection against oxidative damage in this species (Xu et al, 2013a ). Among our DEGs, we identified 14 unigenes belonging to the ascorbate-glutathione cycle, GPX pathway, and Prx/Trx pathway.…”

Section: Resultsmentioning

confidence: 99%

De novo assembly of the Japanese lawngrass (Zoysia japonica Steud.) root transcriptome and identification of candidate unigenes related to early responses under salt stress

Xie

Niu

et al. 2015

Front. Plant Sci.

View full text Add to dashboard Cite

Japanese lawngrass (Zoysia japonica Steud.) is an important warm-season turfgrass that is able to survive in a range of soils, from infertile sands to clays, and to grow well under saline conditions. However, little is known about the molecular mechanisms involved in its resistance to salt stress. Here, we used high-throughput RNA sequencing (RNA-seq) to investigate the changes in gene expression of Zoysia grass at high NaCl concentrations. We first constructed two sequencing libraries, including control and NaCl-treated samples, and sequenced them using the Illumina HiSeq™ 2000 platform. Approximately 157.20 million paired-end reads with a total length of 68.68 Mb were obtained. Subsequently, 32,849 unigenes with an N50 length of 1781 bp were assembled using Trinity. Furthermore, three public databases, the Kyoto Encyclopedia of Genes and Genomes (KEGG), Swiss-prot, and Clusters of Orthologous Groups (COGs), were used for gene function analysis and enrichment. The annotated genes included 57 Gene Ontology (GO) terms, 120 KEGG pathways, and 24 COGs. Compared with the control, 1455 genes were significantly different (false discovery rate ≤0.01, |log2Ratio |≥1) in the NaCl-treated samples. These genes were enriched in 10 KEGG pathways and 73 GO terms, and subjected to 25 COG categories. Using high-throughput next-generation sequencing, we built a database as a global transcript resource for Z. japonica Steud. roots. The results of this study will advance our understanding of the early salt response in Japanese lawngrass roots.

show abstract

“…In a mapping group consisting of 96 zoysiagrass accessions, three SSR (simple sequence repeat) loci and one SRAP (sequence-related amplified polymorphism) locus were found to be associated with cold tolerance, and three SSR loci and two SRAP loci were found to be associated with the green period [ 11 ]. At the protein level, 70 protein spots were found differentially accumulated in stolons after a 14d-cold exposure [ 12 ]. A genome-wide investigation of the gene expression profiles would be helpful for our understanding of the cold response in plants as the gene regulation network is very complex [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Global Transcriptome Profiles of 'Meyer' Zoysiagrass in Response to Cold Stress

Wei

Gao

et al. 2015

PLoS ONE

Self Cite

View full text Add to dashboard Cite

A long green period is essential for a turfgrass species with high ornamental value and a wide area of use. Zoysiagrasses (Zoysia spp. Willd.) are perennial turfgrass species popular in tropical, subtropical and temperate zones, possessing many properties necessary to be economically useful turfgrass. They do not have a long green period because of cold sensitivity. A main focus in zoysiagrass research is to develop cold tolerant cultivars. Understanding the cold response in zoysiagrass is a fundamental area of research. In the present study, ‘Meyer’ zoysiagrass (Zoysia japonica), a widely cultivated variety in the genus, is used. We employed RNA-Seq to investigate genome-wide gene expression profiles in leaves under cold stress (4°C). Using the Illumina sequencing platform, we obtained approximately 206 million high-quality paired-end reads from three libraries (0 h, 2 h, and 72 h cold treatment at 4°C). After de novo assembly and quantitative assessment, 46,412 unigenes were generated with an average length of 998 bp and an N50 of 1,522 bp. A total of 25,644 (55.2%) unigenes were annotated by alignment with public protein databases including NR, SwissProt, KEGG and KOG. Differentially expressed genes (DEGs) were investigated using the RPKM method. A total of 756 DEGs were identified between 0h and 2h-cold treatment, with 522 up-regulated and 234 down-regulated; and 5327 DEGs were identified between 0h and 72h-cold treatment, with 2453 up-regulated and 2874 down-regulated. The expression profile of 15 DEGs selected randomly was confirmed with qRT-PCR. The results suggest that cold stress can induce desiccation and oxidative stress, inhibit photosynthesis and substance transport. In response to the stress, genes involved in proline synthesis, in starch hydrolysis, in methionine and ascorbic acid metabolism, in SOD activity, and in DREBs response pathway were up-regulated. GA metabolism, ABA and JA stimulus response were affected under cold exposure. This is the first transcriptome sequencing of Z. japonica, providing a large set of sequence data as well as gene expression profiles under cold stress. It will improve our current understanding of the cold response of zoysiagrass and be beneficial in breeding research.

show abstract

Comparative Proteomic Analysis of the Stolon Cold Stress Response between the C4 Perennial Grass Species Zoysia japonica and Zoysia metrella

Cited by 36 publications

References 70 publications

Proteomic Analysis of Cold Stress Responses in Banana Leaves

Proteomic Analysis of Cold Stress Responses in Banana Leaves

De novo assembly of the Japanese lawngrass (Zoysia japonica Steud.) root transcriptome and identification of candidate unigenes related to early responses under salt stress

Global Transcriptome Profiles of 'Meyer' Zoysiagrass in Response to Cold Stress

Contact Info

Product

Resources

About