Comparative proteomic analysis provides new insights into cadmium accumulation in rice grain under cadmium stress

Xue, Dawei; Jiang, Hua; Deng, Xiangxiong; Zhang, Xiaoqin; Wang, Hua; Xu, Xiangbin; Hu, Jiang; Zeng, Dali; Guo, Longbiao; Qian, Qian

doi:10.1016/j.jhazmat.2014.08.010

Cited by 98 publications

(26 citation statements)

References 39 publications

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“…Using proteomics strategies, Ahsan et al (2007) identified 21 up-regulated proteins in rice seeds in response to 4-day Cd stress during germination process. Ge et al (2009) identified 13 differential proteins in rice roots and 12 differential proteins in rice leaves under 15-day Cd stress, Lee et al (2010) reported 36 differential proteins in rice roots under 24-h Cd stress, while Xue et al (2014) verified 47 differentially abundant proteins in rice grains at the filling stage under 3 weeks of Cd stress. Moreover, four Cd-induced proteins were reported to be related to tolerance to Cd stress in Shanyou 63 (Ge et al 2009).…”

Section: Introductionmentioning

confidence: 93%

Protein complex and proteomic profile in the roots of Oryza sativa L. in response to cadmium toxicity

et al. 2015

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Cadmium (Cd), a non-essential and toxic heavy metal element, is extremely harmful for rice growth, food safety and even public health. In this paper, two rice varieties, Shanyou 63 (Tolerant to Cd stress) and Nipponbare (Susceptible to Cd stress), were employed as materials to investigate the protein expression and protein-protein interaction in rice in response to different stages of Cd stress. The result showed that Cd accumulated in the root of both rice cultivars, but the Cd content in the root of Shanyou 63 was significantly lower than that in Nipponbare. Eight proteins were up-regulated in the two rice cultivars, but the expressing abundance of these eight proteins in Shanyou 63 was statistically higher than that in Nipponbare, indicating that the relative abundance of these eight proteins was positively related to the ability of rice cultivars to avoid Cd stress. According to Gene Ontology annotation, four of these eight proteins were involved in energy metabolism, while four participated in stress tolerance. Native-PAGE and LC-MS/MS methods revealed that four GSTs, one cysteine synthase and one protein disulfide isomerase formed a stable protein complex in Shanyou 63 root in response to 3-, 5-and 7-day Cd stresses, but this protein complex did not appear in Nipponbare root under Cd stress. Owing to the same domain, the four glutathione S-transferases (GSTs) and cysteine synthase had the potential to interact and compose a protein complex to detoxify Cd in Shanyou 63 rice plants. Our results provide new insight into the detoxification mechanism of rice plant in response to Cd stress and may prove to be useful for the future research.

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Section: Introductionmentioning

confidence: 93%

Protein complex and proteomic profile in the roots of Oryza sativa L. in response to cadmium toxicity

et al. 2015

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“…As global industrialization proceeds, cadmium (Cd) has become one of the most harmful and widespread pollutants in environment (He et al, 2013;Xue et al, 2014). Cadmium is easily taken up by plants and accumulates in edible parts, thus posing risks to humans health through food chains (Uraguchi and Fujiwara, 2013).…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome combined with morpho‐physiological analyses revealed key factors for differential cadmium accumulation in two contrasting sweet sorghum genotypes

Feng

Jia

et al. 2017

Plant Biotechnology Journal

124

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SummaryCadmium (Cd) is a widespread soil contaminant threatening human health. As an ideal energy plant, sweet sorghum (Sorghum bicolor (L.) Moench) has great potential in phytoremediation of Cd‐polluted soils, although the molecular mechanisms are largely unknown. In this study, key factors responsible for differential Cd accumulation between two contrasting sweet sorghum genotypes (high‐Cd accumulation one H18, and low‐Cd accumulation one L69) were investigated. H18 exhibited a much higher ability of Cd uptake and translocation than L69. Furthermore, Cd uptake through symplasmic pathway and Cd concentrations in xylem sap were both higher in H18 than those in L69. Root anatomy observation found the endodermal apoplasmic barriers were much stronger in L69, which may restrict the Cd loading into xylem. The molecular mechanisms underlying these morpho‐physiological traits were further dissected by comparative transcriptome analysis. Many genes involved in cell wall modification and heavy metal transport were found to be Cd‐responsive DEGs and/or DEGs between these two genotypes. KEGG pathway analysis found phenylpropanoid biosynthesis pathway was over‐represented, indicating this pathway may play important roles in differential Cd accumulation between two genotypes. Based on these results, a schematic representation of main processes involved in differential Cd uptake and translocation in H18 and L69 is proposed, which suggests that higher Cd accumulation in H18 depends on a multilevel coordination of efficient Cd uptake and transport, including efficient root uptake and xylem loading, less root cell wall binding, and weaker endodermal apoplasmic barriers.

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“…Rice (Oryza sativa L.) is globally one of the most important cultivated crops feeding nearly half of the world's population [6]. Due to the strong ability of rice plants to absorb Cd, rice Cd pollution is severe [7,8]. The toxicity of Cd in plants leads to photosynthetic damage, a loss of mineral nutrient pathways, and a disruption of carbohydrate metabolism.…”

Section: Introductionmentioning

confidence: 99%

Effects of Melatonin on Growth, Physiology and Gene Expression in Rice Seedlings Under Cadmium Stress

Lv¹,

Fang²,

Zhang³

et al. 2019

Phyton

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Melatonin (MLT) is a hormonal substance found in many organisms and can improve plant stress resistance. In this study, the japonica rice variety Y32 and indica rice variety NJ6 were cultivated in hydroponics under different concentrations of CdCl2 at the two-leaf stage. The growth, physiological and biochemical responses of the seedlings and the expression of cadmium (Cd)related genes under exogenous melatonin (MLT) treatment were assessed. The results indicated that Cd stress destroyed the dynamic balance between reactive oxygen species (ROS) production and removal, resulting in ROS accumulation, membrane lipid peroxidation, and impaired growth and development. Following the application of exogenous MLT to rice seedlings, increases in plant biomass including both underground and above-ground areas were observed. MLT also scavenged the inhibition of superoxide dismutase (SOD) and peroxidase (POD) in a concentration dependent manner in response to Cd stress. Catalase (CAT) activity and malondialdehyde (MDA) expression also decreased following MLT treatment. Amongst the six Cd-related genes assessed, five genes were downregulated and one was up-regulated in response to MLT treatment. Taken together, these data demonstrate that MLT improves the resilience of rice seedlings at the biochemical, physiological, and molecular levels, and diminishes the damage caused by Cd stress.

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Comparative proteomic analysis provides new insights into cadmium accumulation in rice grain under cadmium stress

Cited by 98 publications

References 39 publications

Protein complex and proteomic profile in the roots of Oryza sativa L. in response to cadmium toxicity

Protein complex and proteomic profile in the roots of Oryza sativa L. in response to cadmium toxicity

Comparative transcriptome combined with morpho‐physiological analyses revealed key factors for differential cadmium accumulation in two contrasting sweet sorghum genotypes

Effects of Melatonin on Growth, Physiology and Gene Expression in Rice Seedlings Under Cadmium Stress

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