2019
DOI: 10.3389/fpls.2019.00151
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Comparative Proteomic Analysis Provides New Insights Into Low Nitrogen-Promoted Primary Root Growth in Hexaploid Wheat

Abstract: Nitrogen deficient environments can promote wheat primary root growth (PRG) that allows for nitrogen uptake in deep soil. However, the mechanisms of low nitrogen-promoted root growth remain largely unknown. Here, an integrated comparative proteome study using iTRAQ analysis on the roots of two wheat varieties and their descendants with contrasting response to low nitrogen (LN) stress was performed under control (CK) and LN conditions. In total, 84 differentially abundant proteins (DAPs) specifically involved i… Show more

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Cited by 16 publications
(18 citation statements)
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“…AKTA Purifier system (GE Healthcare, USA) was used for the SCX (strong cationic exchange) chromatography to fractionate the iTRAQ labelled peptides as described previously with minor modifications 6769 . The elution was surveyed using the absorbance at 214 nm.…”
Section: Methodsmentioning
confidence: 99%
See 3 more Smart Citations
“…AKTA Purifier system (GE Healthcare, USA) was used for the SCX (strong cationic exchange) chromatography to fractionate the iTRAQ labelled peptides as described previously with minor modifications 6769 . The elution was surveyed using the absorbance at 214 nm.…”
Section: Methodsmentioning
confidence: 99%
“…In total, 30 fractions were collected (1 fraction/min) and merged into 10 parts, and each part were then desalted and reconstituted in 40 µl 0.1% (v/v) trifluoroacetic acid. A Q Exactive mass spectrometer coupled to an Easy-nLC (Proxeon Biosystems) was used for the LC ESI-MS/MS (liquid chromatography-electrospray ionization tandem MS) analysis according to previous reports 6769 .…”
Section: Methodsmentioning
confidence: 99%
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“…In addition, aminopeptidases are induced under both drought and metal stress in the plant roots (Wang et al 2011 ; Boulila-Zoghlami et al 2011 ). Importantly, aminopeptidases have also been implicated in autophagy under N deficiency (Xia et al 2012 ; Xu et al 2019 ), suggesting that they are a good candidate to investigate for their role in protein-N mobilisation a rhizosphere context.…”
Section: Introductionmentioning
confidence: 99%