Comparative proteomic analysis provides novel insight into the interaction between resistant vs susceptible tomato cultivars and TYLCV infection

Abstract

BackgroundTomato yellow leaf curl virus (TYLCV) is a member of the family Geminiviridae, genus Begomovirus. The virus is a widespread plant virus that causes important economic losses in tomatoes. Genetic engineering strategies have increasingly been adopted to improve the resistance of tomatoes to TYLCV.ResultsIn this study, a proteomic approach was used to investigate the molecular mechanisms involved in tomato leaf defense against TYLCV infection. Proteins extracted from leaves of resistant tomato cultivar ‘Zheza-301’ and susceptible cultivar ‘Jinpeng-1’ after TYLCV infection were analyzed using two-dimensional gel electrophoresis. Eighty-six differentially expressed proteins were identified and classified into seven groups based on their functions. For several of the proteins, including CDC48, CHI and HSC70, expression patterns measured using quantitative real-time PCR differed from the results of the proteomic analysis. A putative interaction network between tomato leaves and TYLCV infection provides us with important information about the cellular activities that are involved in the response to TYLCV infection.ConclusionsWe conducted a comparative proteomic study of TYLCV infection in resistant and susceptible tomato cultivars. The proteins identified in our work show a variety of functions and expression patterns in the process of tomato–TYLCV interaction, and these results contribute to our understanding of the mechanism underlying TYLCV resistance in tomatoes at the protein level.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-016-0819-z) contains supplementary material, which is available to authorized users.

Members of WRKY Group III transcription factors are important in TYLCV defense signaling pathway in tomato (Solanum lycopersicum)

“…Derived from T5678161-1-1-2-2 and T07-018, ‘Zheza-301’ was bred to resist to TYLCV infection with the Ty-2 locus. ‘Jinpeng-1’, a hybrid of Holland tomato cultivar 99-13A and 9708B from America, was susceptible to TYLCV infection [ 6 ]. Seeds of ‘Jinpeng-1’ and ‘Zheza-301’ were obtained from Xi’an Jinpeng Seed Co., Ltd. and the Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, respectively [ 6 ].…”

“…Intact virions pass through the food canal in the stylet of B. tabaci and reach the esophagus; they then move through the plant phloem as B. tabaci inserts its proboscis and feeds on infected tomato leaves [ 4 , 5 ]. Upon entering plant host cells, the DNA of TYLCV replicates via a rolling circular mechanism [ 6 ]. Host enzymes can convert the incoming geminivirus ssDNA to double-stranded DNA (dsDNA) that serves as a transcription template.…”

Proteomic Analysis Reveals the Positive Roles of the Plant-Growth-Promoting Rhizobacterium NSY50 in the Response of Cucumber Roots to Fusarium oxysporum f. sp. cucumerinum Inoculation

“…SAMs catalyze the biosynthesis of S-adenosylmethionine (SAM) from methionine and ATP, and they are active in the biosynthesis of lignin, glycine betaine and polyamines (Fontecave et al, 2004). These proteins can be induced by various environmental stresses, such as cold, salt, drought stress and even pathogen infection (Li et al, 2013a; Huang et al, 2016), and most of them were down-regulated by stress (Wang et al, 2016; Yuan et al, 2016). This outcome was consistent with the results showing that the inoculation of FOC significantly decreased SAMs expression (Table 2, Figure 5C; Supplementary Figures 4, 5).…”

“…Total root protein was isolated from infected cucumber roots of the susceptible bulk and resistant bulk of cucumber generation F2, and the result showed that jasmonic acid and redox signaling components occurred in response to F. oxysporum infection in resistant plants (Zhang et al, 2016). Eighty-six differentially expressed proteins were identified from the leaves of resistant tomato cultivar “Zheza-301” and susceptible cultivar “Jinpeng-1” after TYLCV infection, which demonstrated that an interaction network between tomato leaves and TYLCV infection was established (Huang et al, 2016). To date, although several papers have addressed the use of proteomics to investigate the interactions of plants and pathogens (Konishi et al, 2001; Wang et al, 2011; Carrillo et al, 2014) or plants and PGPR (Kandasamy et al, 2009; Wang et al, 2013), little research is available on the effects of PGPB on cucumber plants under pathogenic attack.…”

Killing two birds with one stone: How do Plant Viruses Break Down Plant Defenses and Manipulate Cellular Processes to Replicate Themselves?

“…In addition to chloroplasts, peroxisomes are a site of ROS production, which is essential for plant defense by HR establishment, local PCD, and activation of plant defense genes. Peroxisomes work in the C2 cycle producing ROS by the activity of glycolate oxidase enzyme (Huang et al 2010;Liu et al 2013;Huang et al 2016). Regarding the two crucial functions displayed by peroxisome in plant defense;…”