Comparative proteomic and transcriptomic approaches to address the active role of GA4 in Japanese apricot flower bud dormancy release

Zhuang, Weibing; Gao, Zhihong; Wang, Liangju; Zhong, Wenjun; Ni, Zhaojun; Zhang, Zhen

doi:10.1093/jxb/ert284

Cited by 106 publications

(98 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results indicated that the accumulation of transcripts and proteins occur independently. A generally low congruency of proteomic and transcriptional profiles has been reported in other previous studies (Lan et al, 2011; Zhuang et al, 2013). …”

Section: Resultssupporting

confidence: 46%

Functional and Integrative Analysis of the Proteomic Profile of Radish Root under Pb Exposure

Wang

Tang

et al. 2016

Front. Plant Sci.

View full text Add to dashboard Cite

Lead (Pb) is one of the most abundant heavy metal (HM) pollutants, which can penetrate the plant through the root and then enter the food chain causing potential health risks for human beings. Radish is an important root vegetable crop worldwide. To investigate the mechanism underlying plant response to Pb stress in radish, the protein profile changes of radish roots respectively upon Pb(NO3)2 at 500 mg L−1(Pb500) and 1000 mg L−1(Pb1000), were comprehensively analyzed using iTRAQ (Isobaric Tag for Relative and Absolute Quantification). A total of 3898 protein species were successfully detected and 2141 were quantified. Among them, a subset of 721 protein species were differentially accumulated upon at least one Pb treatment, and 135 ones showed significantly abundance changes under both two Pb-stressed conditions. Many critical protein species related to protein translation, processing, and degradation, reactive oxygen species (ROS) scavenging, photosynthesis, and respiration and carbon metabolism were successfully identified. Gene Ontology (GO) and pathway enrichment analysis of the 135 differential abundance protein species (DAPS) revealed that the overrepresented GO terms included “cell wall,” “apoplast,” “response to metal ion,” “vacuole,” and “peroxidase activity,” and the critical enriched pathways were involved in “citric acid (TCA) cycle and respiratory electron transport,” “pyruvate metabolism,” “phenylalanine metabolism,” “phenylpropanoid biosynthesis,” and “carbon metabolism.” Furthermore, the integrative analysis of transcriptomic, miRNA, degradome, metabolomics and proteomic data provided a strengthened understanding of radish response to Pb stress at multiple levels. Under Pb stress, many key enzymes (i.e., ATP citrate lyase, Isocitrate dehydrogenase, fumarate hydratase and malate dehydrogenase) involved in the glycolysis and TCA cycle were severely affected, which ultimately cause alteration of some metabolites including glucose, citrate and malate. Meanwhile, a series of other defense responses including ascorbate (ASA)–glutathione (GSH) cycle for ROS scavenging and Pb-defense protein species (glutaredoxin, aldose 1-epimerase malate dehydrogenase and thioredoxin), were triggered to cope with Pb-induced injuries. These results would be helpful for further dissecting molecular mechanism underlying plant response to HM stresses, and facilitate effective management of HM contamination in vegetable crops by genetic manipulation.

show abstract

Section: Resultssupporting

confidence: 46%

Functional and Integrative Analysis of the Proteomic Profile of Radish Root under Pb Exposure

Wang

Tang

et al. 2016

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…There is accumulating evidence of the possible involvement of MADS-box genes and other flowering-related genes in dormancy regulation (Horvath, 2009). Furthermore, recent genome-wide transcriptomic studies have suggested significant roles for phytohormones in the dormancy phase transition of various plant species Díaz-Riquelme et al, 2012;Gai et al, 2013;Habu et al, 2014;Liu et al, 2012;Zhong et al, 2013;Zhuang et al, 2013a). Although this review focused on bud dormancy regulation, dormancy is not only a trait of bud phenology but one of the seasonal growth-regulatory phases for entire trees;…”

Section: Conclusion and Future Studiesmentioning

confidence: 99%

Regulation of Bud Dormancy and Bud Break in Japanese Apricot (Prunus mume Siebold ^|^amp; Zucc.) and Peach [Prunus persica (L.) Batsch]: A Summary of Recent Studies

Yamane

2014

J. Japan. Soc. Hort. Sci.

View full text Add to dashboard Cite

Bud dormancy allows most deciduous fruit tree species to avoid injury in unsuitable environments, synchronize their annual growth, and adapt to a temperate zone climate. Because bud dormancy affects next season's fruit production and vegetative growth, it is considered one of the most important physiological factors that control fruit production. Recent global climate changes require us to better understand the genetic factors regulating bud dormancy, especially those that induce dormancy release and subsequent bud break. In this review, environmental factors that affect the seasonal dormancy depth of Japanese apricot (P. mume Siebold & Zucc.) and peach [P. persica (L.) Batsch] are first outlined. Next, recent progress of genetic, biochemical, and molecular biological studies of Prunus dormancy regulation is described. Recent advances in functional genomics have promoted the discovery of gene function and gene networks associated with bud dormancy regulation. A group of candidate genes for bud dormancy regulation, the DORMANCY-ASSOCIATED MADS-box (DAM) genes in Prunus, are focused. Recently reported expressional analysis suggests a significant role for DAMs in dormancy release and bud break of Japanese apricot and peach vegetative buds. Transformation studies of PmDAM6 have demonstrated that it has an inhibitory effect on the apical growth of poplar (Populus spp.). As bud dormancy is a quantitative polygenic trait, not only DAMs, but also other genes and gene networks appear to regulate bud dormancy. Ongoing and future studies will undoubtedly facilitate the unveiling of the molecular aspects of bud dormancy regulation in temperate fruit tree species of Prunus.

show abstract

“…Rinne et al (2011) consideredthe function of GA 3 and GA 4 in dormancy is distinguished, and only GA 4 can better replace part of the chilling requirement. In the previous studies, our group has proved that GA 4 , as a good dormancy breaker, can replace a part of chilling requirements and cause an early release of dormancy in Japanese apricot (Zhuang et al, 2013). DELLA (Asp-Glu-Leu-Leu-Ala) is a crucial negative regulatory element of the GA signal transduction pathway, which is a subfamily of the GRAS gene family (Salanenka et al, 2018; Nimisha et al, 2013).…”

Section: Introductionmentioning

confidence: 98%

miR169 andPmRGL2synergistically regulate NF-Y complex to active dormancy release in Japanese apricot (Prunus mumeSieb. et Zucc.)

Gao

et al. 2020

Preprint

View full text Add to dashboard Cite

Insufficient chilling requirements affect the floral bud quality and fruit yield in fruit crop production. Endodormancy is a process to meet the chilling requirement. To understand the mechanism of dormancy release in woody plants, we compared the miRNA database during the transition stage from endodormancy to dormancy release in Japanese apricot and found that the miR169 family showed significant differentially up-regulated expression during dormancy and down-regulated during dormancy release periods. The 5’ RACE assay and RT-qPCR validated its target gene NUCLEAR FACTOR-Y subunit A (NF-YA) exhibited the opposite expression pattern. Further study showed that exogenous GA4 could inhibit the expression of PmRGL2 and promote the expression of NF-Y. Moreover, the interaction between NF-Y family and GA inhibitor PmRGL2 was verified by yeast-two-hybrid system and Bimolecular fluorescence complementarity (BiFC) experiment. These results suggested that synergistic regulation of NF-Y and PmRGL2 complex to active dormancy release induced by GA4. These will help to elucidate the functional and regulatory roles of miR169 and its target gene of the seasonal bud dormancy induced by GA4 in Japanese apricot woody plants and to provide new sights for the discovery of dormancy release mechanism.

show abstract

Comparative proteomic and transcriptomic approaches to address the active role of GA4 in Japanese apricot flower bud dormancy release

Cited by 106 publications

References 41 publications

Functional and Integrative Analysis of the Proteomic Profile of Radish Root under Pb Exposure

Functional and Integrative Analysis of the Proteomic Profile of Radish Root under Pb Exposure

Regulation of Bud Dormancy and Bud Break in Japanese Apricot (Prunus mume Siebold ^|^amp; Zucc.) and Peach [Prunus persica (L.) Batsch]: A Summary of Recent Studies

miR169 andPmRGL2synergistically regulate NF-Y complex to active dormancy release in Japanese apricot (Prunus mumeSieb. et Zucc.)

Contact Info

Product

Resources

About