Comparative Proteomics Analyses of Pollination Response in Endangered Orchid Species Dendrobium Chrysanthum

Dendrobium harveyanum is an endangered species of Orchidaceae. Here we report the complete chloroplast (cp) genome sequence and the cp genome features of D. harveyanum. The complete cp genome sequence of D. harveyanum is 157,292 bp in length and presented a typical quadripartite structure including one large single-copy region (LSC, 86,583 bp), one small single-copy region (SSC, 19,449 bp), and two inverted repeat regions (IRs, 25,630 bp each). The cp genome encoded 138 genes, of which 120 were unique genes. The phylogenetic relationships show that D. harveyanum is closely related to other species in Dendrobium.

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“… 2014 ; Wang et al. 2017 ). Dendrobium species have an enormous economic value in global horticultural trade (Teixeira da Silva et al.…”

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confidence: 99%

The complete chloroplast genome of Dendrobium harveyanum (Orchidaceae)

Huang

Pan

Chen

et al. 2019

Mitochondrial DNA Part B

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“…By elucidating developmental processes in orchids, omics approaches can assist with breeding, genetic improvement, conservation, and commercial production in orchids. Comparative proteomics analyses of pollination response in the endangered orchid species Dendrobium chrysanthum were carried out [218]. For a better understanding of the mechanism of pollination in D. chrysanthum, the differentially expressed proteins (DEP) between the self-pollination and cross-pollination pistil of D. chrysanthum were investigated via two-dimensional electrophoresis (2-DE) coupled with tandem mass spectrometry [218].…”

Section: Computational and Omics Approaches In Orchid Biologymentioning

confidence: 99%

“…Comparative proteomics analyses of pollination response in the endangered orchid species Dendrobium chrysanthum were carried out [218]. For a better understanding of the mechanism of pollination in D. chrysanthum, the differentially expressed proteins (DEP) between the self-pollination and cross-pollination pistil of D. chrysanthum were investigated via two-dimensional electrophoresis (2-DE) coupled with tandem mass spectrometry [218]. A total of 54 DEP spots were identified in the two-dimensional electrophoresis (2-DE) maps between the self-pollination and cross-pollination.…”

Section: Computational and Omics Approaches In Orchid Biologymentioning

confidence: 99%

“…Gene ontology analysis revealed an array of proteins belonging to the functional categories: metabolic process (8.94%), response to stimulus (5.69%), biosynthetic process (4.07%), protein folding (3.25%), and transport (3.25%). The identification of these DEPs at the early response stage of pollination provides new insights into the mechanism of pollination response and assists in the conservation of the orchid species [218]. By proteomics techniques (LC-MS/MS, LTQ (HPLC)), flower labellum tissues sampled from Ophrys exaltata subsp.…”

Section: Computational and Omics Approaches In Orchid Biologymentioning

confidence: 99%

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Advances in Orchid Biology: Biotechnological Achievements, Translational Success, and Commercial Outcomes

Tiwari,

Sharma,

Bose

et al. 2024

Horticulturae

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Orchids constitute the largest and most diverse group of flowering plants and are classified in the family Orchidaceae. Exhibiting significance as the most exotic and ubiquitous flowering plant, the cultivation of orchids on a commercial level is gaining momentum worldwide. In addition to its ornamental and aesthetic value, the orchid industry has successfully generated employment for people in developing countries. Recent advances in biotechnological interventions in orchids have substantially contributed to the development of exotic varieties with novel traits, not to forget the inputs of traditional plant breeding methods and tissue culture approaches. In addition, the scientific developments in orchid biology have remarkably bridged the knowledge gaps in areas of orchid classification, phytochemistry, and cultivation strategies. This has facilitated the commercialization of novel varieties, opening new avenues in the orchid industry, and their global marketing as cut flowers and artificially propagated plants. Orchids constitute the first floriculture crops that revolutionized the orchid industry; however, they also hold several challenges in the natural propagation and conservation of several species that are on the verge of extinction. International organizations like CITES have come forward to address challenges associated with illegal global trade and indiscriminate use of orchid varieties, aiming for conservation and legal commercial goals. This thematic review is one-of-a-kind in providing comprehensive insights into the emerging momentum of orchid biology and how its globalization projects to considerably impact the orchid industry in the coming times. However, it is imperative to understand the challenges in the cultivation and conservation of orchid varieties and ensure legislative guidelines both on domestic and global levels to ensure a multipronged approach to the conservation and commercialization of orchids.

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“…Proteomic analyses of pollination in Dendrobium. chrysanthum exhibited that β-oxidation of fatty acids, amino acid metabolism, oxidative phosphorylation, and ubiquitin-related protein were associated with SI [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Cytological Observation and Transcriptome Comparative Analysis of Self-Pollination and Cross-Pollination in Dendrobium Officinale

Chen

Zhang

et al. 2021

Genes

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Dendrobium officinale is a rare and traditional medicinal plant with high pharmacological and nutritional value. The self-incompatibility mechanism of D. officinale reproductive isolation was formed in the long-term evolution process, but intraspecific hybridization of different germplasm resources leads to a large gap in the yield, quality, and medicinal value of D. officinale. To investigate the biological mechanism of self-incompatibility in D. officinale, cytological observation and the transcriptome analysis was carried out on the samples of self-pollination and cross-pollination in D. officinale. Results for self-pollination showed that the pollen tubes could grow in the style at 2 h, but most of pollen tubes stopped growing at 4 h, while a large number of cross-pollinated pollen tubes grew along the placental space to the base of ovary, indicating that the self-incompatibility of D. officinale may be gametophyte self-incompatibility. A total of 63.41 G basesum of D. officinale style samples from non-pollinated, self-pollination, and cross-pollination by RNA-seq were obtained, and a total of 1944, 1758, and 475 differentially expressed genes (DEGs) in the comparison of CK (non-pollinated) vs. HF (cross-pollination sample), CK vs. SF (self-pollination sample) and SF vs. HF were identified, respectively. Forty-one candidate genes related to self-incompatibility were found by function annotation of DEGs, including 6 Ca2+ signal genes, 4 armed repeat containing (ARC) related genes, 11 S-locus receptor kinase (SRK) related genes, 2 Exo70 family genes, 9 ubiquitin related genes, 1 fatty acid related gene, 6 amino acid-related genes, 1 pollen-specific leucine-rich repeat extensin-like protein (LRX) related gene and 1 lectin receptor-like kinases (RLKs) related gene, showed that self-incompatibility mechanism of D. officinale involves the interaction of multiple genes and pathways. The results can provide a basis for the study of the self-incompatibility mechanism of D. officinale, and provide ideas for the preservation and utilization of high-quality resources of D. officinale.

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Comparative Proteomics Analyses of Pollination Response in Endangered Orchid Species Dendrobium Chrysanthum

Cited by 6 publications

References 75 publications

The complete chloroplast genome of Dendrobium harveyanum (Orchidaceae)

The complete chloroplast genome of Dendrobium harveyanum (Orchidaceae)

Advances in Orchid Biology: Biotechnological Achievements, Translational Success, and Commercial Outcomes

Cytological Observation and Transcriptome Comparative Analysis of Self-Pollination and Cross-Pollination in Dendrobium Officinale

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