Comparative proteomics analysis of OsNAS1 transgenic Brassica napus under salt stress

Abstract

Salt stress is one of the major abiotic stresses in agricultural plants worldwide . We used proteomics to analyze the differential expression of proteins in transgenic OsNAS1 and non-transformant Brassica napus treated with 20 mmol/L Na 2 CO 3 . Total protein from the leaves was extracted and separated through a high-resolution and highly repetitive two-dimensional electrophoresis (2-DE) technology system. Twelve protein spots were reproducibly observed to be upregulated by more than 2-fold between transgenic and non-transformant B. napus. These 12 spots were digested in-gel with trypsin and characterized by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to obtain the peptide mass fingerprints. Protein database searching revealed that 5 of these proteins are involved in salt tolerance: dehydrogenase, glutathione S-transferase, peroxidase, 20S proteasome beta subunit, and ribulose-1,5-bisphosphate carboxylase/oxygenase. The potential functions of these identified proteins in substance and energy metabolism, stress tolerance, protein degradation, and cell defense are discussed. The salt tolerance of the transgenic rapeseed was significantly improved by the introduction of the OsNAS1 gene from Brazilian upland rice of Oryza sativa (cv. IAPAR 9). Brassica napus, nicotianamine synthase (NAS), salt-stress, differential proteins Citation:Kong F, Mao S J, Du K, et al. Comparative proteomics analysis of OsNAS1 transgenic Brassica napus under salt stress.

Comparative transcriptome and translatome analysis in contrasting rice genotypes reveals differential mRNA translation in salt-tolerant Pokkali under salt stress

“…For instance, two nicotianamine synthase (NAS) genes were repressed at the transcriptional level but upregulated at the translational level in Pok during salt stress. In plants, NAS can catalyze and synthesize niacinamide (NA), which participates in iron ion transportation, distribution and storage, as well as transportation of other heavy metal ions [26]. Overexpression of OsNAS1 in Brassica napus enhanced the protein level of five salt stress-related genes, including dehydrogenase, glutathione S-transferase, peroxidase, 20S proteasome beta subunit, and ribulose-1,5-bisphosphate carboxylase/oxygenase, and significantly improved the plant salt tolerance [26].…”

“…Alternative splicing is another important gene regulatory mechanism associated with plant stress responses [56]. The TFs and other genes associated with stress tolerance were reported to undergo alternative splicing in Arabidopsis and other plants [20, 26, 57, 58]. In rice, salt stress has been reported to change the expression of alternatively spliced AOX [20, 59].…”

Changes in SeMSC, Glucosinolates and Sulforaphane Levels, and in Proteome Profile in Broccoli (Brassica oleracea var. Italica) Fertilized with Sodium Selenate

“…In particular, B. napus exposed to phosphorus deficiency showed changes in the synthesis of some proteins related with gene transcription, carbon metabolism, energy transfer, stress-responses and defense against pathogens [ 16 ]. In addition, transgenic B. napus that over-expressed nicotin amine synthase gene exposed to sodium excess showed changes in the synthesis of some proteins involved in salt tolerance, energy metabolism and defense [ 17 ]. Moreover, B. napus subjected to boron deprivation showed changes in the synthesis of proteins involved in carbohydrate and energy metabolism, stress response, amino acid and nucleic acid metabolism, among others [ 18 ].…”

Omics: The way forward to enhance abiotic stress tolerance inBrassica napusL

“…It also reduced the H 2 O loss, improve biomass, and yield production under stress conditions. 192 The hyperactive expression of the Arabidopsis AtDWF4 gene elevated seed productivity and tolerance to heat, drought, dehydration stresses and enhances the seed yield in transgenic lines. 195 The cloning of tobacco serine acetyltransferase (SAT; a rate-limiting enzyme for cysteine biosynthesis)-encoding gene ( NtSAT4 ) generated high levels of glutathione and cysteine in transgenic rapeseed lines for improved heavy metals tolerance.…”