Comparative proteomics analysis of the responses to selenium in selenium-enriched alfalfa (Medicago sativa L.) leaves

Transcriptome analysis revealed accumulation‐assimilation of selenium and physio‐biochemical changes in alfalfa (Medicago sativa L.) leaves

“…Alfalfa ( Medicago sativa L. cv. Kangsai), a high Se accumulation variety, 5 was planted at the Henan Agricultural University field experimental station in XinXiang (113°N, 35°E) Henan, China, in 2020. The content of organic matter in the soil was 18.10 g kg −1 (NY/T1121.6‐2006), pH was 8.87 (NY/T1377‐2007), total nitrogen content was 1.160 g kg −1 (HJ717‐2014), total phosphorus content was 0.078% (NY/T88‐1988), total potassium content was 3.97 × 10 −1 (NY/T87‐1988), available nitrogen content was 1.1610 × 10 −1 (DB13/T843‐2007), available phosphorus content was 1.147 × 10 −2 g kg −1 (NY/T1121.7–2014), available potassium content was 1.47 × 10 −1 g kg −1 (NY/T889‐2004), effective Se content was 4.8 × 10 −4 g kg −1 (NY/T3420‐2019).…”

“…Here, sodium selenite (Na 2 SeO 3 ) as a common Se(IV) was selected as foliar fertilization to study the effect of Se biofortification in alfalfa according to previous studies. The accumulation and assimilation of Se(IV) in alfalfa needs to be further studied, though some important results have been found in preliminary research 5 …”

“…With the rapid development of domestic animal husbandry in China and other developing countries, the demand for high‐quality forage, especially alfalfa, has increased dramatically. Furthermore, in the Se fortification of alfalfa, the addition of Se promoted the yield and increased the Se content to 40 mg kg −1 5 . Alfalfa being a good model plant has a bright future of Se enrichment in green biorefining 6 …”

“…The accumulation and assimilation of Se(IV) in alfalfa needs to be further studied, though some important results have been found in preliminary research. 5 Selenium(VI) is similar to sulfur (S) in chemical properties, and it is usually accumulated with the help of the way S transports in some plants (Phaseolus vulgaris 10 and Oryza sativa 11 ). Although Se(VI) is increasingly used in crops as foliar Se fertilizer, the absorption of Se(VI) remains inconclusive.…”

“…Furthermore, in the Se fortification of alfalfa, the addition of Se promoted the yield and increased the Se content to 40 mg kg −1 . 5 Alfalfa being a good model plant has a bright future of Se enrichment in green biorefining. 6 The process of Se enrichment in plants is affected by ways of Se application, the forms of Se, and the contents of Se application.…”