Comparative Quantitative Assessments of Salivary Ion Activity Product for Hydroxyapatite and Buffering Capacity in Children with Different Caries Experience

Anand, Siddharth; Masih, Updesh; Yeluri, Ramakrishna

doi:10.17796/1053-4628-40.6.480

Cited by 4 publications

(4 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The intervention in preschool children with the daily drinking of milk supplemented with Lactobacillus rhamnosus and Bifidobacteruim longum improved the buffer capacity of saliva in a consistent way, contributing to mitigate this risk factor for the appearance and progression of ECC 2 . No studies were found in preschoolers that compared the salivary buffering capacity after an intervention of milk with probiotics, nevertheless, the advantages of incorporating probiotics into dairy products because of the buffering capacity of the food matrix and the presence of milk components are found in the correlation with tooth enamel remineralization 30 .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial

et al. 2018

View full text Add to dashboard Cite

Probiotics have provided benefits to general health, but they are still insufficient to dental health.Objective:This study aimed to evaluate milk supplemented with probiotic bacteria and standard milk, measured by levels of Streptococcus mutans (S. mutans) and Lactobacillus spp., in 3-4-year-old children after 9 months of intervention.Material and Methods:The study was a triple-blind, placebo-controlled, randomized trial. The sample was composed of 363 preschoolers attending five child development centers in Cali, Colombia. They were randomized to two groups: children in the intervention group drank 200 mL of milk with Lactobacillus rhamnosus 5x10 6 and Bifidobacteruim longum 3x10 6 , and children in the control group drank 200 mL of standard milk. Interventions occurred on weekdays and information was gathered through scheduled clinical examination. The primary result was the number of colony forming units (CFU) of S. mutans and Lactobacillus spp. in the saliva. Secondary results were dental caries, rated by the International Caries Detection and Assessment System (ICDAS), dental plaque, pH, and salivary buffer capacity.Results:The proportion of S. mutans was lower in the intervention group compared with the control group after 9 months; however, the differences did not reach statistical significance (p=0.173); on the other hand, statistically significant differences between groups were found in the CFU/mL of Lactobacillus spp. (p=0.002). There was not statistically significant difference in the prevalence of dental caries for both groups (p=0.767). Differences between groups were found in the salivary buffering capacity (p=0.000); neither salivary pH nor dental plaque were significantly different.Conclusions:Regular consumption of milk containing probiotics bacteria reduced CFU/mL of Lactobacillus spp. and increased salivary buffering capacity at 9 months of consumption.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The dependent variables were the changes of S. mutans and Lactobacillus spp. A Mann Whitney U test for numerical variables and X 2 test for categorical variables was performed.…”

Section: Methodsmentioning

confidence: 99%

Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial

et al. 2018

View full text Add to dashboard Cite

show abstract

“…One of the most relevant caries-protective factors in saliva is the buffer capacity, given mainly by bicarbonate (HCO 3 -). The bicarbonate system stands out because when an acid is added to the oral environment, it reacts releasing a weak carbonic acid that rapidly dissociates into water and CO 2 , resulting in a complete elimination and increasing the pH of the oral environment, following the chemical balance: H + + HCO 3 -⇋ H 2 CO 3 ⇋ H 2 O + CO 2 [30]. The phosphate and the protein buffers are also important to enhance the buffer capacity in saliva.…”

Section: Discussionmentioning

confidence: 99%

Saliva Decreases Sucrose-induced Cariogenicity in an Experimental Biological Caries Model

Giacaman¹,

Umaña²,

Núñez³

et al. 2023

Preprint

View full text Add to dashboard Cite

Objective. Whether a minimum quantity of saliva is needed to inhibit the caries process is unclear. Here, we evaluated the effect of saliva dilutions on an in vitro caries model with Streptococcus mutans (S. mutans) biofilms. Methods. S. mutans biofilms were grown on enamel and root dentin slabs, in culture media containing different proportions of saliva (v/v): 0%, 5%, 10%, 25%, 50%, 75% and 100% saliva, and exposed to a 10% sucrose solution (5 min, 3x/day), with appropriate controls. After 5 (enamel) and 4 (dentin) days, demineralization, biomass, viable bacteria and polysaccharide formation were analyzed. The acidogenicity of the spent media was monitored overtime. Assays were carried out in triplicate in 2 independent experiments (n=6). Results. An inverse relationship was observed between acidogenicity, demineralization, and saliva proportion, both in enamel and dentin. A marked reduction in enamel and dentin demineralization was observed when small amounts of saliva were incorporated in the medium. For both tissues, a significant reduction in biomass, viable S. mutans cells and polysaccharides were observed when saliva was present, in a concentration-dependent manner. Conclusions. High quantities of saliva can almost completely inhibit sucrose-induced cariogenicity, but small amounts can also exert a dose-dependent caries-protective effect.

show abstract

“…One of the most relevant caries-protective factors in saliva is the buffer capacity, given mainly by bicarbonate (HCO 3 − ). The bicarbonate system stands out because when an acid is added to the oral environment, it reacts releasing a weak carbonic acid that rapidly dissociates into water and CO 2 , resulting in a complete elimination and increasing the pH of the oral environment, following the chemical balance: H + + HCO 3 − ⇋ H 2 CO 3 ⇋ H 2 O + CO 2 [ 7 ]. The phosphate and the protein buffers are also important to enhance the buffer capacity in saliva.…”

Section: Introductionmentioning

confidence: 99%

Saliva Decreases Sucrose-Induced Cariogenicity in an Experimental Biological Caries Model

et al. 2023

View full text Add to dashboard Cite

Objective. Whether a minimum quantity of saliva inhibit the caries process remains uncertain. This study aimed to investigate the impact of saliva dilutions on an in vitro caries model using Streptococcus mutans (S. mutans) biofilms. Methods. S. mutans biofilms were cultivated on enamel and root dentin slabs, in culture media containing different proportions of saliva (v/v): 0%, 5%, 10%, 25%, 50%, 75%, and 100% saliva, and exposed to a 10% sucrose solution (5 min, 3x/day), with appropriate controls. After 5 (enamel) and 4 (dentin) days, demineralization, biomass, viable bacteria, and polysaccharide formation were analyzed. The acidogenicity of the spent media was monitored overtime. Each assay was performed in triplicate across two independent experiments (n = 6). Results. In both enamel and dentin, an inverse relationship was observed between acidogenicity, demineralization, and the proportion of saliva. Even small quantities of saliva incorporated into the media led to a noticeable reduction in enamel and dentin demineralization. Saliva presence resulted in significant reductions in biomass, viable S. mutans cells, and polysaccharides, with the effects being concentration-dependent for both tissues. Conclusions. High quantities of saliva can almost completely inhibit sucrose-induced cariogenicity, while even small amounts exhibit a dose-dependent caries-protective effect.

show abstract

Comparative Quantitative Assessments of Salivary Ion Activity Product for Hydroxyapatite and Buffering Capacity in Children with Different Caries Experience

Abstract: pH measurement after HCl titration in saliva could be used as chair side screening test for the assessment of caries risk.

Cited by 4 publications

References 1 publication

Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial

Effects of a food enriched with probiotics on Streptococcus mutans and Lactobacillus spp. salivary counts in preschool children: a cluster randomized trial

Saliva Decreases Sucrose-induced Cariogenicity in an Experimental Biological Caries Model

Saliva Decreases Sucrose-Induced Cariogenicity in an Experimental Biological Caries Model

Contact Info

Product

Resources

About