In this study, a novel quad-band antenna for biomedical applications was designed, fabricated and analyzed. Biomedical application defines the use of antenna in detecting cancerous cells and its cure using hyperthermia. In this research paper, a rectangular micro strip patch antenna is modified with the circular and pentagonal shapes of negative media (Metamaterial). Antenna was reduced in size and ameliorated to operate on multiple frequency bands. Ameliorated antenna is operating at 1GHz also, which is the substantial operating frequency of cancer detecting tissues. Inclusion of metamaterial increased the bandwidth of and subsequently ameliorate the size of the proposed antenna.