Comparative response of epithelial cells and osteoblasts to microfabricated tapered pit topographies in vitro and in vivo

Hamilton, David A.; Chehroudi, B.; Brunette, D. M.

doi:10.1016/j.biomaterials.2007.01.026

Cited by 79 publications

(82 citation statements)

References 31 publications

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“…1 Upon its placement in the jaw, an implant must interface successfully with three types of cells, including osteoblasts from the bone and epithelial cells plus the underlying fibroblasts from the soft tissue. 2,3 Among these cells, osteoblasts secrete matrix proteins and deposit bone mineral for osteogenesis and osseointegration to withstand occlusal loading, whereas epithelial cells and fibroblasts adhere to the transmucosal component to form a barrier to protect the underlying bone tissues. 3,4 Moreover, implant surfaces are exposed to a bacteria-rich environment and rapidly become colonized by oral bacteria that can compete with epithelial and connective tissues and cells for binding to the implant surface.…”

Section: Introductionmentioning

confidence: 99%

“…2,3 Among these cells, osteoblasts secrete matrix proteins and deposit bone mineral for osteogenesis and osseointegration to withstand occlusal loading, whereas epithelial cells and fibroblasts adhere to the transmucosal component to form a barrier to protect the underlying bone tissues. 3,4 Moreover, implant surfaces are exposed to a bacteria-rich environment and rapidly become colonized by oral bacteria that can compete with epithelial and connective tissues and cells for binding to the implant surface. 5 Bacterial accumulation may result in infection, destruction of the tissueimplant integration or even implant failure; accordingly, inhibiting bacterial adhesion would be conducive to the success and survival of implants.…”

Section: Introductionmentioning

confidence: 99%

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The response of human osteoblasts, epithelial cells, fibroblasts, macrophages and oral bacteria to nanostructured titanium surfaces: a systematic study

Miao¹,

Wang²,

Xu³

et al. 2017

IJN

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Nanotopography modification is a major focus of interest in current titanium surface design; however, the influence of the nanostructured surface on human cell/bacterium behavior has rarely been systematically evaluated. In this study, a homogeneous nanofiber structure was prepared on a titanium surface (Nano) by alkali-hydrothermal treatment, and the effects of this Nano surface on the behaviors of human MG-63 osteoblasts, human gingival epithelial cells (HGECs) and human gingival fibroblasts (HGFs) were evaluated in comparison with a smooth titanium surface (Smooth) by polishing and a micro-rough titanium surface (Micro) by sandblasting and acid etching. In addition, the impacts of these different surface morphologies on human THP-1 macrophage polarization and Streptococcus mutans attachment were also assessed. Our findings showed that the nanostructured surface enhanced the osteogenic activity of MG-63 cells (Nano=Micro>Smooth) at the same time that it improved the attachment of HGECs (Nano>Smooth>Micro) and HGFs (Nano=Micro>Smooth). Furthermore, the surface with nanotexture did not affect macrophage polarization (Nano=Micro=Smooth), but did reduce initial bacterial adhesion (Nano

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The response of human osteoblasts, epithelial cells, fibroblasts, macrophages and oral bacteria to nanostructured titanium surfaces: a systematic study

Miao¹,

Wang²,

Xu³

et al. 2017

IJN

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show abstract

“…28,30,31,45,46 Perhaps not surprisingly, a completely different geometric nanoporous surface nurtured primary rodent and human podocytes, on which arborized cell processes were formed similarly to those traditionally obtained by collagen coating. In this sense, podocytes, despite the presence of numerous ramifications, display a similar behavior to that of other epithelial cell types whose attachment and spreading seem to be discouraged by rough surfaces 47,48 and facilitated instead by nanoporous materials. 49 This behavior was not unexpected as it indirectly confirms what is known about the geometry of the GBM surface obtained by SEM studies performed after freeze-fracture procedures optimized to get a view of the GBM itself.…”

mentioning

confidence: 85%

A nanoporous surface is essential for glomerular podocyte differentiation in three-dimensional culture

Zennaro¹,

Rastaldi²,

Bakeine³

et al. 2016

IJN

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Although it is well recognized that cell–matrix interactions are based on both molecular and geometrical characteristics, the relationship between specific cell types and the three-dimensional morphology of the surface to which they are attached is poorly understood. This is particularly true for glomerular podocytes – the gatekeepers of glomerular filtration – which completely enwrap the glomerular basement membrane with their primary and secondary ramifications. Nanotechnologies produce biocompatible materials which offer the possibility to build substrates which differ only by topology in order to mimic the spatial organization of diverse basement membranes. With this in mind, we produced and utilized rough and porous surfaces obtained from silicon to analyze the behavior of two diverse ramified cells: glomerular podocytes and a neuronal cell line used as a control. Proper differentiation and development of ramifications of both cell types was largely influenced by topographical characteristics. Confirming previous data, the neuronal cell line acquired features of maturation on rough nanosurfaces. In contrast, podocytes developed and matured preferentially on nanoporous surfaces provided with grooves, as shown by the organization of the actin cytoskeleton stress fibers and the proper development of vinculin-positive focal adhesions. On the basis of these findings, we suggest that in vitro studies regarding podocyte attachment to the glomerular basement membrane should take into account the geometrical properties of the surface on which the tests are conducted because physiological cellular activity depends on the three-dimensional microenvironment.

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“…Another report demonstrated that mineral deposition by osteoblasts was enhanced on the surfaces containing pits in vitro and in vivo [62]. Although both these studies were performed on different materials and using different cells, they are in line with the mild positive effect of the pits observed in our study.…”

Section: Discussionsupporting

confidence: 77%

Divide, combine and conquer : on the effect of individual properties of biomaterials for bone regeneration

Danoux¹

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Comparative response of epithelial cells and osteoblasts to microfabricated tapered pit topographies in vitro and in vivo

Cited by 79 publications

References 31 publications

The response of human osteoblasts, epithelial cells, fibroblasts, macrophages and oral bacteria to nanostructured titanium surfaces: a systematic study

The response of human osteoblasts, epithelial cells, fibroblasts, macrophages and oral bacteria to nanostructured titanium surfaces: a systematic study

A nanoporous surface is essential for glomerular podocyte differentiation in three-dimensional culture

Divide, combine and conquer : on the effect of individual properties of biomaterials for bone regeneration

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