Comparative results of preimplantation genetic screening by array comparative genomic hybridization and new-generation sequencing

Aleksandrova, N. V.; Shubina, Jekaterina; Ekimov, Ekimov A.N.; Kodyleva, T. A.; Mukosey, I. S.; Makarova, N. P.; Kulakova, E. V.; Levkov, Lev; Barkov, I. Yu.; Trofimov, Trofimov D.Yu.; Сухих, Г. Т.

doi:10.1134/s0026893317010022

Cited by 2 publications

(2 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The karyotype evaluation was performed for the selected embryos of the 1st and 2nd classes, which are the most likely to be transferred during the single embryo transfer protocol. Blastocyst trophectoderm biopsy was performed for the preimplantation genetic testing for aneuploidy (PGT-A), and karyotype quality was assessed by array comparative genomic hybridization [ 74 ]). After the biopsy, day 5 embryos were cryopreserved and stored until transfer.…”

Section: Methodsmentioning

confidence: 99%

LC-MS Analysis Revealed the Significantly Different Metabolic Profiles in Spent Culture Media of Human Embryos with Distinct Morphology, Karyotype and Implantation Outcomes

Eldarov

Gamisonia

Chagovets

et al. 2022

IJMS

View full text Add to dashboard Cite

In this study we evaluated possible differences in metabolomic profiles of spent embryo culture media (SECM) of human embryos with distinct morphology, karyotype, and implantation outcomes. A total of 153 samples from embryos of patients undergoing in vitro fertilization (IVF) programs were collected and analyzed by HPLC-MS. Metabolomic profiling and statistical analysis revealed clear clustering of day five SECM from embryos with different morphological classes and karyotype. Profiling of day five SECM from embryos with different implantation outcomes showed 241 significantly changed molecular ions in SECM of successfully implanted embryos. Separate analysis of paired SECM samples on days three and five revealed 46 and 29 molecular signatures respectively, significantly differing in culture media of embryos with a successful outcome. Pathway enrichment analysis suggests certain amino acids, vitamins, and lipid metabolic pathways to be crucial for embryo implantation. Differences between embryos with distinct implantation potential are detectable on the third and fifth day of cultivation that may allow the application of culture medium analysis in different transfer protocols for both fresh and cryopreserved embryos. A combination of traditional morphological criteria with metabolic profiling of SECM may increase implantation rates in assisted reproductive technology programs as well as improve our knowledge of the human embryo metabolism in the early stages of development.

show abstract

Section: Methodsmentioning

confidence: 99%

LC-MS Analysis Revealed the Significantly Different Metabolic Profiles in Spent Culture Media of Human Embryos with Distinct Morphology, Karyotype and Implantation Outcomes

Eldarov

Gamisonia

Chagovets

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…However, only large scale IVF centers can afford the expense of equipment and software, the strong skills and knowledge of professionals including analysis and interpretation of bioinformatics data [11]. The transport scheme sounds feasible for small and average scale laboratories.…”

Section: Introductionmentioning

confidence: 99%

The Reported Successful Baby Delivery After Preimplantation Genetic Testing for Aneuploidies (Pgt-A) by Means of Next Generation Sequencing (Ngs)

Nigmatova

Abdilmanova

Schigolev³

2020

View full text Add to dashboard Cite

The aim of this case report is to demonstrate a successful delivery of a baby after transfer of a blastocyst tested for aneuplodies by means of NGS. A woman aged 35 having two miscarriages decided for ICSI program with PGT-A analysis. Six eggs were fertilized out of 9 metaphase II oocytes. Five good quality blastocysts were submitted for genetic screening using 24-chromosome next generation sequencing (NGS). Two blastocysts were diagnosed as euploid and recommended for transfer. One euploid blastocyst was thawed and transferred to the patient’s uterus lining. Successful pregnancy was confirmed at 7 weeks of gestation with heartbeat. Successful delivery was achieved by Caesarean section at 38-39 weeks of gestation. Karyotyping demonstrated healthy genetic constitution of a baby. This case demonstrates a good evidence and potential of a transport scheme collaboration between IVF and genetic laboratories.

show abstract

Comparative results of preimplantation genetic screening by array comparative genomic hybridization and new-generation sequencing

Cited by 2 publications

References 18 publications

LC-MS Analysis Revealed the Significantly Different Metabolic Profiles in Spent Culture Media of Human Embryos with Distinct Morphology, Karyotype and Implantation Outcomes

LC-MS Analysis Revealed the Significantly Different Metabolic Profiles in Spent Culture Media of Human Embryos with Distinct Morphology, Karyotype and Implantation Outcomes

The Reported Successful Baby Delivery After Preimplantation Genetic Testing for Aneuploidies (Pgt-A) by Means of Next Generation Sequencing (Ngs)

Contact Info

Product

Resources

About