Comparative RNA-Seq analysis reveals a critical role for brassinosteroids in rose (Rosa hybrida) petal defense against Botrytis cinerea infection

Liu, Xintong; Cao, Xiaoqian; Shi, Shaochuan; Zhao, Na; Li, Dandan; Fang, Peihong; Chen, Xi; Qi, Weicong; Zhang, Zhao

doi:10.1186/s12863-018-0668-x

Cited by 65 publications

(72 citation statements)

References 51 publications

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“…Auxin levels decreased in P. patens after B. cinerea infection, while ABA levels increases at 24 hpi, although this hormone could be of fungal origin used as a mechanism to contribute to infection (Ponce de León et al 2012). BR are important for rose defense against B. cinerea (Liu et al, 2018), and here we show that all fungal-responsive DEGs of this pathway are upregulated in P. patens. However, the BR receptor and the key regulator BKI1 (Brassinosteroid Insensitive1 Kinase Inhibitor)…”

Section: Discussionsupporting

confidence: 63%

Transcriptional profiling reveals conserved and species-specific plant defense responses during the interaction of the early divergent plantPhyscomitrium patenswithBotrytis cinerea

Reboledo

Agorio

Vignale

et al. 2020

Preprint

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Bryophytes were among the first plants that colonized earth and they evolved key defense mechanisms to counteract microbial pathogens present in the new environment. Although great advances have been made on pathogen perception and subsequent defense activation in angiosperms, limited information is available in early divergent land plants. In this study, a transcriptomic approach uncovered the molecular mechanisms underlying the defense response of the bryophyte Physcomitrium patens against the important plant pathogen Botrytis cinerea. A total of 3.072 differentially expressed genes were significantly affected during B. cinerea infection, including genes encoding proteins with known function in angiosperm immunity and involved in pathogen perception, signaling, transcription, hormonal signaling, metabolic pathways such as shikimate and phenylpropanoid, and proteins with diverse role in defense against biotic stress. Similarly as in other plants, B. cinerea infection leads to downregulation of genes involved in photosynthesis and cell cycle progression. These results highlight the existence of evolutionary conserved defense responses to pathogens throughout the green plant lineage, suggesting that they were probably present in the common ancestors of land plants. Moreover, several genes acquired by horizontal transfer from prokaryotes and fungi, and a high number of P. patens-specific orphan genes were differentially expressed during B. cinerea infection, indicating that they are part of the moss immune response and probably played an ancestral role related to effective adaptation mechanisms to cope with pathogen invasion during the conquest of land.

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Section: Discussionsupporting

confidence: 63%

Transcriptional profiling reveals conserved and species-specific plant defense responses during the interaction of the early divergent plantPhyscomitrium patenswithBotrytis cinerea

Reboledo

Agorio

Vignale

et al. 2020

Preprint

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“…Recently, transcriptome, metabolome, and proteome analyses have been applied as powerful tools to explain the plant-pathogen interactions in many plants, such as Zymoseptoria tritici [7], Fusarium culmorum [8], Botrytis cinerea [9], Bradyrhizobium diazoefficiens [10], Candidatus Liberibacter asiaticus [11], Xanthomonas oryzae [12], and Cucumber mosaic virus [13].…”

Section: Introductionmentioning

confidence: 99%

Integrated Transcriptomic and Un-Targeted Metabolomics Analysis Reveals Mulberry Fruit (Morus atropurpurea) in Response to Sclerotiniose Pathogen Ciboria shiraiana Infection

Bao

Gao

Zheng

et al. 2020

IJMS

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Mulberry sclerotiniose caused by Ciboria shiraiana is a devastating disease of mulberry (Morus alba L.) fruit in Northwest China. At present, no disease-resistant varieties are used in production, as the molecular mechanisms of this disease are not well understood. In this study, to explore new prevention methods and provide direction for molecular breeding, transcriptomic sequencing and un-targeted metabolomics were performed on healthy (CK), early-stage diseased (HB1), and middle-stage diseased (HB2) mulberry fruits. Functional annotation, gene ontology, a Kyoto encyclopedia of genes and genomes (KEGG) analysis, and a Mapman analysis of the differentially expressed genes revealed differential regulation of genes related to plant hormone signal transduction, transcription factors, and phenylpropanoid biosynthesis. A correspondence between the transcript pattern and metabolite profile was observed in the phenylpropanoid biosynthesis pathway. It should be noted that the log2 ratio of eugenol (isoeugenol) in HB1 and HB2 are 85 times and 23 times higher than CK, respectively. Our study shows that phenylpropanoid biosynthesis may play an essential role in response to sclerotiniose pathogen infection and eugenol(isoeugenol) enrichment in mulberry fruit, which may provide a novel method for mulberry sclerotiniose control.

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“…This involves the upregulation of WRKY expression upon pathogen infection. To study the RcWRKY responses to B. cinerea, we obtained RNA-seq transcriptomic data from rose petals exposed to this pathogen at 30 hours post inoculation (hpi) and 48 hpi [10]. In rose petals, B. cinerea conidia germinate at 24 hpi, and the early response to infection is considered to occur at 30 dpi, as no visible disease lesions form by this point.…”

Section: Expression Patterns Of the Rcwrky Genes In Response To B CImentioning

confidence: 99%

“…have not yet been identified. We previously explored the molecular basis of rose resistance against B. cinerea using a de novo RNA-Seq analysis, revealing that large numbers of genes, including WRKY family genes, were significantly upregulated in roses upon B. cinerea infection [10]. In the present study, we performed a genome-wide analysis of the WRKY family in roses, and used virus-induced gene silencing (VIGS) to confirm that RcWRKY41 plays an important role in rose resistance against gray mold.…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide characterization of the rose (Rosa chinensis) WRKY family and role of RcWRKY41 in gray mold resistance

Liu

Zhang

et al. 2019

Preprint

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Background The WRKYs are a major family of plant transcription factors that play roles in the responses to biotic and abiotic stresses; however, a comprehensive study of the WRKY family in roses ( Rosa sp.) has not previously been performed.Results In the present study, we performed a genome-wide analysis of the WRKY genes in the rose ( Rosa chinensis ), including their phylogenetic relationships, gene structure, chromosomal locations, and collinearity. Using a phylogenetic analysis, we divided the 56 RcWRKY genes into three subgroups. The RcWRKY s were unevenly distributed across all seven rose chromosomes, and a study of their collinearity suggested that genome duplication may have played a major role in RcWRKY gene duplication. A Ka/Ks analysis indicated that they mainly underwent purifying selection. Botrytis cinerea infection induced the expression of 19 RcWRKY s, most of which had undergone gene duplication during evolution. These RcWRKY s may regulate rose resistance against B. cinerea . Based on our phylogenetic and expression analyses, RcWRKY41 was identified as a candidate regulatory gene in the response to B. cinerea infection, which was confirmed using virus-induced gene silencing.Conclusions This study provides useful information to facilitate the further study of the function of the rose WRKY gene family.

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Comparative RNA-Seq analysis reveals a critical role for brassinosteroids in rose (Rosa hybrida) petal defense against Botrytis cinerea infection

Cited by 65 publications

References 51 publications

Transcriptional profiling reveals conserved and species-specific plant defense responses during the interaction of the early divergent plantPhyscomitrium patenswithBotrytis cinerea

Transcriptional profiling reveals conserved and species-specific plant defense responses during the interaction of the early divergent plantPhyscomitrium patenswithBotrytis cinerea

Integrated Transcriptomic and Un-Targeted Metabolomics Analysis Reveals Mulberry Fruit (Morus atropurpurea) in Response to Sclerotiniose Pathogen Ciboria shiraiana Infection

Genome-wide characterization of the rose (Rosa chinensis) WRKY family and role of RcWRKY41 in gray mold resistance

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