Comparative Role of Immunoglobulin A in Protective Immunity against the Bordetellae

Wolfe, Daniel; Kirimanjeswara, Girish S.; Goebel, Elizabeth M.; Harvill, Eric T.

doi:10.1128/iai.00412-07

Cited by 38 publications

(57 citation statements)

References 43 publications

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“…While strain RB50-induced sera recognized several antigens common to strains RB50, 1289, and RB50⌬wbm, antibodies in this sera also recognized a broad smear of antigens (most prominent between 15 and 37 kDa) present in strain RB50 but not strain RB50⌬wbm (Fig. 1A), which confirms previous reports showing that this smear is O antigen (42). The O-antigen smear is also absent in the lysate of strain 1289, which suggests that this strain either does not express O antigen or expresses an antigenically distinct Oantigen serotype (Fig.…”

Section: Bordetella Bronchiseptica Strains Express Antigenically Distsupporting

confidence: 78%

“…Western blotting was performed essentially as described previously (6,42 ELISAs. Enzyme-linked immunosorbent assay (ELISA) experiments were carried out essentially as described previously (28,41).…”

Section: Methodsmentioning

confidence: 99%

“…Additionally, while B. bronchiseptica and B. parapertussis express an O antigen, the wbm locus has been deleted from B. pertussis, resulting in a lack of O-antigen synthesis (30,32). The O antigens of B. bronchiseptica and B. parapertussis are highly antigenic (41,42), increase their resistance to complementmediated killing (7,10), and contribute to the ability of these species to colonize the lower respiratory tract of mice (7,10).…”

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confidence: 99%

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Evidence for Horizontal Gene Transfer of Two Antigenically Distinct O Antigens in Bordetella bronchiseptica

et al. 2009

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Host immunity is a major driving force of antigenic diversity, resulting in pathogens that can evade immunity induced by closely related strains. Here we show that two Bordetella bronchiseptica strains, RB50 and 1289, express two antigenically distinct O-antigen serotypes (O1 and O2, respectively). When 18 additional B. bronchiseptica strains were serotyped, all were found to express either the O1 or O2 serotype. Comparative genomic hybridization and PCR screening showed that the expression of either the O1 or O2 serotype correlated with the strain containing either the classical or alternative O-antigen locus, respectively. Multilocus sequence typing analysis of 49 B. bronchiseptica strains was used to build a phylogenetic tree, which revealed that the two O-antigen loci did not associate with a particular lineage, evidence that these loci are horizontally transferred between B. bronchiseptica strains. From experiments using mice vaccinated with purified lipopolysaccharide from strain RB50 (O1), 1289 (O2), or RB50⌬wbm (O antigen deficient), our data indicate that these O antigens do not confer cross-protection in vivo. The lack of cross-immunity between O-antigen serotypes appears to contribute to inefficient antibody-mediated clearance between strains. Together, these data are consistent with the idea that the O-antigen loci of B. bronchiseptica are horizontally transferred between strains and encode antigenically distinct serotypes, resulting in inefficient cross-immunity.

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Section: Bordetella Bronchiseptica Strains Express Antigenically Distsupporting

confidence: 78%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Evidence for Horizontal Gene Transfer of Two Antigenically Distinct O Antigens in Bordetella bronchiseptica

et al. 2009

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“…B. parapertussis strain CN2591 and an isogenic mutant lacking O antigen, CN2591⌬wbm, have previously been described (42) and were maintained on Bordet-Gengou agar (Difco, Sparks, MD) containing 10% sheep's blood (Hema Resources, Aurora, OR) with 20 g/ml streptomycin. For inoculation, the bacteria were grown overnight at 37°C in Stainer-Scholte broth (26,54) to mid-log phase and diluted in phosphate-buffered saline (PBS; OmniPur, Gibbstown, NJ) to a concentration of 1.0 ϫ 10 7 CFU/ml (23,28,60,61).…”

Section: Methodsmentioning

confidence: 99%

O Antigen Protects Bordetella parapertussis from Complement

et al. 2008

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Bordetella pertussis, a causative agent of whooping cough, expresses BrkA, which confers serum resistance, but the closely related human pathogen that also causes whooping cough, Bordetella parapertussis, does not. Interestingly, B. parapertussis, but not B. pertussis, produces an O antigen, a factor shown in other models to confer serum resistance. Using a murine model of infection, we determined that O antigen contributes to the ability of B. parapertussis to colonize the respiratory tract during the first week of infection, but not thereafter. Interestingly, an O antigen-deficient strain of B. parapertussis was not defective in colonizing mice lacking the complement cascade. O antigen prevented both complement component C3 deposition on the surface and complement-mediated killing of B. parapertussis. In addition, O antigen was required for B. parapertussis to systemically spread in complement-sufficient mice, but not complement-deficient mice. These data indicate that O antigen enables B. parapertussis to efficiently colonize the lower respiratory tract by protecting against complement-mediated control and clearance.The major component of the outer leaflet of gram-negative bacteria, lipopolysaccharide (LPS), is composed of three major regions; a lipid A, a core oligosaccharide, and an O polysaccharide (O antigen) (12). Most biological effects of LPS have been attributed to the immunostimulatory properties of lipid A (35, 44); however, O antigen plays important roles in protection against host immune mechanisms, such as complementmediated killing, and antimicrobial peptide-mediated bactericidal effects (9,24,38,39,45,48,52,53). For example, the shortened O antigen of serum-sensitive strains of Pseudomonas aeruginosa is associated with increased C3 deposition (47). The presence of O antigen on Klebsiella pneumoniae LPS appears to have no effect on C3 deposition or its ability to cause pneumonia, although its presence does increase serum resistance in vitro (1, 16). In addition, P. aeruginosa and Yersinia entercolitica O antigens affect the expression and/or proper function of other virulence factors (4,6,10,38). These examples illustrate that there is considerable variation in the function of the O antigen portion of the LPS among different bacterial pathogens (12,35).Bordetella parapertussis and Bordetella pertussis are the causative agents of whooping cough (34). Although these pathogens are very closely related (17,36,56), there is substantial variation in LPS structures between them (2, 3, 17, 42, 43). B. pertussis produces a lipo-oligosaccharide containing lipid A and a branched-chain core oligosaccharide with a complex trisaccharide modification, but completely lacks O antigen due to a 20-kb deletion in the wbm locus responsible for O-antigen synthesis (36). B. parapertussis produces an LPS molecule that has a distinct lipid A and a core oligosaccharide lacking the trisaccharide modification, but includes an O antigen (42,43,55). Interestingly, both of these pathogens are commonly found in the human popul...

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“…Local nasal immunity (IgA) appears to be of crucial importance in respiratory infectious diseases, particularly if Bordetella spp. is implicated [17,23,32,33].…”

Section: Vaccinesmentioning

confidence: 99%

Bordetella Bronchiseptica Vaccines in Pet Guinea Pigs? A Review of the Literature

Gallego¹

2017

SOJVS

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Bordetella bronchiseptica causes severe respiratory disease in guinea pigs and it has been associated with stillbirths, abortions, infertility and infections of the reproductive system and tympanic bullae. Disease caused by Bordetella bronchiseptica in pet guinea pigs is mostly a poor understood disease; studies about this pathogen has been conducted mostly in laboratory guinea pigs. The objective of this review is to condense published information about the disease caused by Bordetella bronchiseptica in guinea pigs for the veterinarian who attends pet guinea pigs, with special emphasis on the prevention of this disease. The author concludes that vaccination in pet guinea pigs against Bordetella bronchiseptica needs to be considered for future studies. Received PathogenyBordetella bronchiseptica (Bb) is a gram-negative rod that has tropism for the ciliated respiratory epithelium. Infected pet guinea pigs can eliminate the bacteria, become asymptomatic carriers or develop the disease. The pet guinea pigs that develop the disease usually are attended at the veterinary clinic showing nasal and ocular discharge, respiratory sounds and dyspnea; the inappetence and lethargy indicate a poor prognosis. The diagnosis and treatment is based on deep nasal culture and antibiogram. Guinea pigs that have contact with the diseased guinea pig should be separated and occurrence of clinical signs should be monitorized at least until the diseased guinea pig has been cured. It is good practice to perform x-rays of the head and chest of the guinea pigs that are in contact with the diseased ones because a subclinical or early-stage disease can be detected [1][2][3].Bordetella bronchiseptica causes severe respiratory disease in guinea pigs, firstly described at the end of the 19th century [4] (Figure 1). Stillbirths, abortions, infertility and infections of the reproductive system can occur if haematonous spread of the bacteria develops; also the tympanic bullae may contain purulent exudates in affected animals due to ascending infection [1,5,6] (Figure 2,3). The incidence of Bb in a colony is usually 20% lower in laboratory animals if no action is taken, but this incidence is unknown in guinea pigs [7]. Disease caused by Bb in pet guinea pigs is mostly a poor understood disease because what is known of the disease is mostly by studies conducted in laboratory guinea pigs, and not in pet guinea pigs [1][2][3].

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Comparative Role of Immunoglobulin A in Protective Immunity against the Bordetellae

Cited by 38 publications

References 43 publications

Evidence for Horizontal Gene Transfer of Two Antigenically Distinct O Antigens in Bordetella bronchiseptica

Evidence for Horizontal Gene Transfer of Two Antigenically Distinct O Antigens in Bordetella bronchiseptica

O Antigen Protects Bordetella parapertussis from Complement

Bordetella Bronchiseptica Vaccines in Pet Guinea Pigs? A Review of the Literature

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