Comparative Salt-Responses of Excised Cotyledons and Seedlings of Pea to Various Osmotic and Ionic Stresses

Guerrier, Gilles; Patolia, J.S.

doi:10.1016/s0176-1617(89)80128-2

Cited by 25 publications

(10 citation statements)

References 35 publications

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“…Na + and K + concentrations were determined using a flame spectrophotometer (Eppendorf flame photometer, PHF 90D, LeMans, France). The Ca 2+ concentration was quantified by an atomic absorption spectrophotometer (Shimadzu AA-6200, Kyoto, Japan) (Guerrier and Patolia 1989). For Cl − content estimation, ions were extracted with hot distilled water (80°C) during 2 h. Chloride was determined (Buchler-Cotlove chloridometer) as described by Taleisnik et al (1997).…”

Section: Methodsmentioning

confidence: 99%

Salt resistance of tomato species grown in sand culture

Doğan¹,

Tıpırdamaz²,

Demir³

2010

Plant Soil Environ.

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In this study, Na + , Cl -, K + , Ca 2+ , chlorophyll and proline levels and the rate of lipid peroxidation level in terms of malondialdehyde (MDA), were investigated in tissues of 15 different tomato cultivars in salt tolerance. As a material, 15 different tomato genotypes were used during a 28-day period and 150 mmol NaCl was applied in sand culture. While one of tomato genotypes was a wild type belonging to Lycopersicum peruvianum, the others were local genotypes belonging to Lycopersicum esculentum L. Better NaCl-stress tolerance in salt-tolerant cultivars as compared to salt-sensitive cultivars observed during the present investigation might be due to restriction of Na + accumulation and ability to maintain high K + /Na + ratio in tissue. The chlorophyll level decreased more in salt-sensitive than in salt-resistant cultivars, whereas proline level increased more in salt-sensitive than in salt-resistant cultivars. The exposure to NaCl induced a significant increase in MDA level in both salt-resistant and salt-sensitive cultivars; yet, MDA level was higher in salt-sensitive cultivars. As a result, exclusion or inclusion of Na + , Cl -, K + and Ca 2+ MDA levels, chlorophyll and proline levels may play a key protective role against stress and these features can be used as identifiers for tolerance to salt.

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Section: Methodsmentioning

confidence: 99%

Salt resistance of tomato species grown in sand culture

Doğan¹,

Tıpırdamaz²,

Demir³

2010

Plant Soil Environ.

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“…Ca 2+ measurements were conducted by means of an atomic absorption spectrophotometer according to the method by Issac (1980). Cl − was colorimetrically determined with ferric ammonium sulfate and mercuric thiocyanate following the procedure by Guerrier and Patolia (1989)). Every measurement was replicated three times, and the mean was used for analysis.…”

Section: Analysis Of Soluble Sugarsmentioning

confidence: 99%

Variation in Populus euphratica foliar carbon isotope composition and osmotic solute for different groundwater depths in an arid region of China

Feng

et al. 2015

Environ Monit Assess

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Water use efficiency (WUE) is an important trait associated with plant acclimation caused by water deficits, and δ13C is a good surrogate of WUE under conditions of water deficits. Water deficiency also enhances the accumulation of compatible solutes in the leaves. In this study, variations in foliar δ(13)C values and main osmotic solutes were investigated. Those included total soluble sugar (TSS), sucrose, free proline, glycine betaine (GB), and inorganic ionic (K+, Ca2+, and Cl-) content of Populus euphratica for different groundwater depths in a Ejina desert riparian forest, China. Results indicated that foliar δ13C values in the P. euphratica for different groundwater depths ranged from -29.14±0.06 to -25.84±0.04 ‰. Foliar δ13C signatures became richer as groundwater levels declined. TSS, sucrose, free proline, GB, and K+ were accumulated in P. euphratica foliage with developing plant growth and increasing groundwater depth. Ca2+ and Cl- content increased under stronger P. euphratica transpiration rates for shallower groundwater depths (1-2.5 m) and decreased for deeper groundwater depths (greater than 3.0 m). Moreover, correlations between δ13C, osmotic solutes, and groundwater depths showed that the primary osmotic solutes were TSS, sucrose, proline, GB, and K+. Correlations also showed that δ13C was not only a useful measure for P. euphratica-integrated WUE but also could be used as an indicator reflecting some physiological osmotic indexes.

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“…Chloride was estimated on frozen material using the ferric ammonium sulphate and mercuric thiocyanate colorimetric method according to Guerrier and Patolia (1989). Ion concentrations were expressed as μmol g −1 DW.…”

Section: Ion Analysismentioning

confidence: 99%

The role of cotyledon metabolism in the establishment of quinoa (Chenopodium quinoa) seedlings growing under salinity

Ruffino¹,

Rosa²,

Hilal³

et al. 2009

Plant Soil

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A growth chamber experiment was conducted to assess the effect of salinity on emergence, growth, water status, photosynthetic pigments, osmolyte accumulation, and ionic content of quinoa seedlings (Chenopodium quinoa). The aim was to test the hypothesis that quinoa seedlings are well adapted to grow under salinity due to their ability to adjust the metabolic functionality of their cotyledons. Seedlings were grown for 21 days at 250 mM NaCl from the start of the germination. Germination percentage and cotyledon area were not affected by salt whereas seedling height decreased 15%. FW increased in both control and salt-treated cotyledons, but the increase was higher under salinity. DW only increased in salttreated cotyledons. The DW/FW ratio did not show significant differences between treatments. Relative water content, chlorophyll, carotenoids, lipids, and proteins were significantly lower under salinity. Total soluble sugars, sucrose and glucose concentrations were higher in salt-treated than in control cotyledons. Ion concentration showed a different distribution pattern. Na + and Cl − concentrations were higher under salinity, while an inverse result was observed for K + concentration. Proline and glycinebetaine concentrations increased under salinity, but the increase was higher in the former than the latter. The osmoprotective role of proline, glycinebetaine, and soluble sugars is discussed.

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Comparative Salt-Responses of Excised Cotyledons and Seedlings of Pea to Various Osmotic and Ionic Stresses

Cited by 25 publications

References 35 publications

Salt resistance of tomato species grown in sand culture

Salt resistance of tomato species grown in sand culture

Variation in Populus euphratica foliar carbon isotope composition and osmotic solute for different groundwater depths in an arid region of China

The role of cotyledon metabolism in the establishment of quinoa (Chenopodium quinoa) seedlings growing under salinity

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