Comparative secretome analysis of Trichoderma asperellum S4F8 and Trichoderma reesei Rut C30 during solid-state fermentation on sugarcane bagasse

Abstract

BackgroundThe lignocellulosic enzymes of Trichoderma species have received particular attention with regard to biomass conversion to biofuels, but the production cost of these enzymes remains a significant hurdle for their commercial application. In this study, we quantitatively compared the lignocellulolytic enzyme profile of a newly isolated Trichoderma asperellum S4F8 strain with that of Trichoderma reesei Rut C30, cultured on sugarcane bagasse (SCB) using solid-state fermentation (SSF).ResultsComparison of the lignocellulolytic enzyme profiles of S4F8 and Rut C30 showed that S4F8 had significantly higher hemicellulase and β-glucosidase enzyme activities. Liquid chromatography tandem mass spectrometry analysis of the two fungal secretomes enabled the detection of 815 proteins in total, with 418 and 397 proteins being specific for S4F8 and Rut C30, respectively, and 174 proteins being common to both strains. In-depth analysis of the associated biological functions and the representation of glycoside hydrolase family members within the two secretomes indicated that the S4F8 secretome contained a higher diversity of main and side chain hemicellulases and β-glucosidases, and an increased abundance of some of these proteins compared with the Rut C30 secretome.ConclusionsIn SCB SSF, T. asperellum S4F8 produced a more complex lignocellulolytic cocktail, with enhanced hemicellulose and cellobiose hydrolysis potential, compared with T. reesei Rut C30. This bodes well for the development of a more cost-effective and efficient lignocellulolytic enzyme cocktail from T. asperellum for lignocellulosic feedstock hydrolysis.

Assessment of the Effect of Easily-metabolised Carbon Supplements on Xylanase Production by Newly Isolated Trichoderma asperellum USM SD4 Cultivated on Oil Palm Empty Fruit Bunches

“…Xylanase activities reported in earlier studies were very poor in the range 14. U/gds (Marx et al 2013) and 162 U/mL (Asad et al 2015), which is the highest xylanase activity ever reported prior to this study. Finding in this study therefore suggested the potential of T. asperellum for enhanced xylanase production Growth rate and the corresponding xylanase productivity by T. asperellum USM SD4 were determined.…”

“…Notwithstanding that xylanase is a principal fraction of several cell-walls degrading enzymes by which it exerts its mycoparasitic property, most studies on T. asperellum have focused on its bio-control ability at the expense of other industrial uses. Recently, both Bech et al (2015) and Marx et al (2013) separately reported a detailed proteomics of the secretome by T. asperellum to show xylanase as an important component enzyme that could be exploited for important industrial processes. However, despite their respective strides, the potential of T. asperellum as a suitable fungal isolate for enhanced xylanase has not been well exploited.…”

On-Site Enzyme Production by Trichoderma asperellum for the Degradation of Duckweed

“…Other factors that improve cellulase production by T. reesei include relative humidity and temperature [28,29], continuous light exposure [27] and aeration and higher substrate concentration (Pandey, 2003) [30]. In a study performed on T. asperellum, a marginally higher endoglucanase and β-xylosidase activity was measured when it was incubated in darkness under a controlled relative humidity of 90%, compared with the standard culture without relative humidity control [10]. In the contrary, exposure to continuous light generally had a negative effect on the activity of endoxylanases and side chain hemicellulases [10].…”

“…In a study performed on T. asperellum, a marginally higher endoglucanase and β-xylosidase activity was measured when it was incubated in darkness under a controlled relative humidity of 90%, compared with the standard culture without relative humidity control [10]. In the contrary, exposure to continuous light generally had a negative effect on the activity of endoxylanases and side chain hemicellulases [10]. This study focused on the substrate composition by changing the growth substrate to correlate it to enzyme production and enzyme diversity.…”

“…Several Trichoderma species have been successfully cultivated on various lignocellulosic substrates, and their Carbohydrate Active enZymes (CAZymes) analyzed [7,8]. Trichoderma asperellum has shown its potential for secreting a range of GHs [9,10], growing on substrates such as cellulose [11] and sugar bagasse [10]. This paper will focus on the degradation of duckweed, a common name for the Lemnaceae family, is a small flowering plant, belonging to a family of five genera (Lemna, Landoltia, Spirodela, Wolffia and Wolfiella) and 38 species.…”