Comparative secretomics reveals novel virulence-associated factors of Vibrio parahaemolyticus

Abstract

Vibrio parahaemolyticus is a causative agent of serious human seafood-borne gastroenteritis disease and even death. In this study, for the first time, we obtained the secretomic profiles of seven V. parahaemolyticus strains of clinical and food origins. The strains exhibited various toxic genotypes and phenotypes of antimicrobial susceptibility and heavy metal resistance, five of which were isolated from aquatic products in Shanghai, China. Fourteen common extracellular proteins were identified from the distinct secretomic profiles using the two-dimensional gel electrophoresis (2-DE) and liquid chromatography tandem mass spectrometry (LC-MS/MS) techniques. Of these, half were involved in protein synthesis and sugar transport of V. parahaemolyticus. Strikingly, six identified proteins were virulence-associated factors involved in the pathogenicity of some other pathogenic bacteria, including the translation elongation factor EF-Tu, pyridoxine 5′-phosphate synthase, σ54 modulation protein, dihydrolipoyl dehydrogenase, transaldolase and phosphoglycerate kinase. In addition, comparative secretomics also revealed several extracellular proteins that have not been described in any bacteria, such as the ribosome-recycling factor, translation elongation factor EF-Ts, phosphocarrier protein HPr and maltose-binding protein MalE. The results in this study will facilitate the better understanding of the pathogenesis of V. parahaemolyticus and provide data in support of novel vaccine candidates against the leading seafood-borne pathogen worldwide.

Comparative Proteomics and Secretomics Revealed Virulence and Antibiotic Resistance-Associated Factors in Vibrio parahaemolyticus Recovered From Commonly Consumed Aquatic Products

“…The secretomic profiles of the V. parahaemolyticus ATCC33847 and ATCC17802 strains of clinical origin were also obtained for comparative secretomic analysis, although they have been reported in our prior research (He et al, 2015). One interesting observation was that the toxic ATCC33847 and ATCC17802 strains appeared to secret more extracellular proteins (59-62) than the V. parahaemolyticus isolates recovered from the 12 species of aquatic products (17-35), consistent with our prior results (He et al, 2015).…”

“…Extracellular proteins of V. parahaemolyticus isolates were extracted according to the method described previously (He et al, 2015) with minor modifications. Briefly, growth curves of V. parahaemolyticus isolates incubated in the TSB medium (pH 8.5, 3% NaCl) at 37 • C were determined using Bioscreen Automatic Growth Curve Analyzer (BioTek Instruments, Inc., Winooski, VT, United States).…”

“…In our prior researches, V. parahaemolyticus contamination in 10 species of aquatic products was surveyed (He et al, 2016;Hu and Chen, 2016). Secretomic profiles of seven V. parahaemolyticus strains of two clinical and five aquatic product origins (Haliotis asinina, Moerella iridescens, Metapenaeus ensis, Exopalaemon carinicauda, and L. vannamei) were compared, and six virulence-associated proteins involved in bacterial pathogenicity were identified (He et al, 2015). Recently, a number of V. parahaemolyticus isolates from 25 species of commonly consumed shellfish, crustacean, and fish samples were isolated and characterized (Su et al, unpublished).…”

Comparative genomics for non-O1/O139 Vibrio cholerae isolates recovered from the Yangtze River Estuary versus V. cholerae representative isolates from serogroup O1

“…Genomic DNA was prepared using the Biospin Bacteria DNA Extraction Kit (BIOER Technology, Hangzhou, China) and plasmid DNA was isolated using the TaKaRa MiniBEST Plasmid Purification Kit Version 3.0 (Japan TaKaRa BIO, Dalian Company, China) according to the manufacturers' instructions. Three independently prepared DNA samples were examined by agarose gel electrophoresis, visualised and imaged as described previously (He et al 2015b). No plasmid DNA was extracted from CHN108B.…”

A Comprehensive Epidemiological Research for Clinical Vibrio parahaemolyticus in Shanghai

“…The researches for V. parahaemolyticus strains isolated from aquatic products are widely reported (Wang et al, 2011; Guo et al, 2013; He et al, 2015, 2016; Lou et al, 2016; Hu and Chen, 2016; Yu et al, 2016; Zhang et al, 2017), while studies on clinical isolates has been poorly documented (Zhang and Orth, 2013; Qi et al, 2016). …”