Comparative sensitivity of three methods for the diagnosis of cytomegalovirus lung infection

Stirk, P. R.; Griffiths, PD

doi:10.1016/0166-0934(88)90147-4

Cited by 12 publications

(3 citation statements)

References 13 publications

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“…In contrast, cell culture is more sensitive than immunofluorescence staining for detection of influenza and parainfluenza viruses and should be performed if the immunofluorescence test is negative (47,108,117,147). Other uses of virus-specific antibodies include rapid diagnosis of measles by detection of viral antigen in smears of nasopharyngeal cells (124), diagnosis of CMV pneumonia by detection of virus-infected cells in smears of bronchoalveolar lavage fluid (166), and differentiation of asymptomatic shedding of CMV during reactivation from active disease. The last is based on detection and quantitation of antigenemia by staining smears of peripheral leukocytes (buffy coat) with a monoclonal antibody against CMV pp65 structural antigen (15,179).…”

Section: Direct Detection In Smearsmentioning

confidence: 99%

Detection of infection or infectious agents by use of cytologic and histologic stains

1996

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A wide variety of stains are useful for detection of different organisms or, for viruses, the cytopathologic changes they induce, in smears prepared directly from clinical specimens and in tissue sections. Other types of stains, such as hematoxylin and eosin, are used routinely to stain tissue sections and are most valuable for assessing the immunologic response of the host to the invading pathogen. In many cases, the pattern of inflammation provides important clues to diagnosis and helps to guide the selection of additional "special" stains used predominantly for diagnosis of infectious diseases. A stain may be nonspecific, allowing detection of a spectrum of organisms, as do the Papanicolaou stain and silver impregnation methods, or detection of only a limited group of organisms, as do the different acid-fast techniques. Some nonspecific stains, such as the Gram stain, are differential and provide valuable preliminary information concerning identification. Immunohistochemical stains, on the other hand, are specific for a particular organism, although in some cases cross-reactions with other organisms occur. Despite the wealth of information that can be gleaned from a stained smear or section of tissue, however, the specific etiology of an infection often cannot be determined on the basis of only the morphology of the organisms seen; culture data are essential and must be considered in the final diagnosis.

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Section: Direct Detection In Smearsmentioning

confidence: 99%

Detection of infection or infectious agents by use of cytologic and histologic stains

1996

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“…In the immunocompromised host, rapid diagnosis of cytomegalovirus (CMV) disease is often critical for patient management, particularly with the availability of investigational antiviral agents that show promise for the treatment of CMV disease (1,3,4,13,15). Recently, the use of bronchoalveolar lavage (BAL) has been shown to be highly sensitive and specific for the diagnosis of CMV pneumonia in bone marrow transplant patients (2,5,6,12,17). Rapid identification of CMV in such specimens by the clinical laboratory is desirable to facilitate patient management.…”

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confidence: 99%

Direct detection of cytomegalovirus from bronchoalveolar lavage samples by using a rapid in situ DNA hybridization assay

et al. 1989

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An in situ DNA hybridization assay was compared with centrifugation culture for rapid detection of cytomegalovirus (CMV) from bronchoalveolar lavage (BAL) samples. Eighty BAL samples were inoculated into both centrifugation culture and standard culture. Cytospin preparations of the BAL samples were studied in a 75-min in situ DNA hybridization assay using the PathoGene CMV kit (Enzo Biochem, Inc., New York, N.Y.). Of the 80 samples, 39 (49%) were positive for CMV; 37 of 39 (95%) were positive by centrifugation culture, 34 of 39 (87%) were positive in standard culture, 24 of 39 (62%) were positive by in situ hybridization, and 20 of 39 (56%) were positive by histologic and/or immunofluorescence techniques. The in situ hybridization assay detected 23 of the 37 samples positive in centrifugation culture, for a sensitivity of 62% and a specificity of 98%. We conclude that the in situ hybridization assay is a specific and more rapid test than centrifugation culture and standard culture for diagnosis of CMV pulmonary infection. For the clinical laboratory, however, current hybridization methods are not sufficiently sensitive to replace centrifugation culture for detection of CMV in BAL specimens.

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“…George et al, 1993;Woods et al, 1990). Moreover, cytology and direct detection of either CMV antigen or DNA in BAL cells are insensitive as diagnostic tests for CMV pneumonia (Eriksson et al, 1993;Gleaves et al, 1989;Martin and Smith, 1986;Stirk and Griffiths, 1988). Finally, although polymerase chain reaction (PCR) is highly sensitive for the detection of CMV DNA in lung, it cannot easily distinguish nonpathogenic CMV infection from CMV disease.…”

Section: Introductionmentioning

confidence: 97%

Clinical utility of CMV early and late transcript detection with NASBA in bronchoalveolar lavages

Keightley

Rinaldo

Bullotta

et al. 2006

Journal of Clinical Virology

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Comparative sensitivity of three methods for the diagnosis of cytomegalovirus lung infection

Cited by 12 publications

References 13 publications

Detection of infection or infectious agents by use of cytologic and histologic stains

Detection of infection or infectious agents by use of cytologic and histologic stains

Direct detection of cytomegalovirus from bronchoalveolar lavage samples by using a rapid in situ DNA hybridization assay

Clinical utility of CMV early and late transcript detection with NASBA in bronchoalveolar lavages

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