Comparative sensitivity of three new cell lines developed from gill, liver and brain tissues of goldfish, Carassius auratus (L.) to cyprinid herpesvirus-2 (CyHV-2)

Swaminathan, T. Raja; Raja, Arun; Dharmaratnam, Arathi; Nithianantham, Sundar Raj

doi:10.1016/j.jviromet.2021.114069

Cited by 5 publications

(4 citation statements)

References 28 publications

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“…S2-S21), which suggest these cultures have stable genomes. Both primary cultures and cell lines have potential to be used in several fields, such as cytogenetics, genomics, pathology, immunology, toxicology, cellular agriculture and conservation (Swaminathan et al 2021;Goswami et al 2022).…”

Section: Establishment Of Continuous Ray-finned Fish Cell Culturementioning

confidence: 99%

“…The in vitro culture of cells is fundamental for several fields of biological research, being an alternative for studies that use animals, since it mimics the cellular homogeneity of the original organisms beyond the advantage of reducing the variability of responses observed under in vivo conditions (Segeritz and Vallier 2017). In the case of fish cell cultures, besides being an important biomedical tool, they can offer important contributions to aquaculture and fish conservation (Swaminathan et al 2021;Goswami et al 2022). According to the Cellosaurus database (Bairoch 2018) there are 850 lines of ray-finned fishes to date, representing 196 species included in 27 orders (Fig.…”

Section: Introductionmentioning

confidence: 99%

“…S1). These cell lines are derived from different organs and species, being fin, kidney, embryo, brain and gonad the most used organs (Swaminathan et al 2021).…”

Section: Introductionmentioning

confidence: 99%

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Universal conditions for establish continuous cell cultures in ray-finned fishes

Cardoso,

Nascimento Oliveira,

Climaco

et al. 2023

Preprint

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Ray-finned fishes represent the most diverse vertebrate lineage and show extensive variations in physiology, ways of life and adaptations to marine and freshwater environments. Actinopterygii are largely exploited for human consumption and several species have been established as biological models. The in vitro culture of cells is fundamental for several fields of biological research, being an alternative for studies that use animals, since it mimics the cellular homogeneity of the original organisms beyond the advantage of reducing the variability of responses observed under in vivo conditions. In the case of fish cell cultures, besides being an important biomedical tool, they can offer important contributions to aquaculture and fish conservation. Hundreds of fish cell lines have been established using specific methods for each cell type and species. Here we describe an optimized protocol for obtaining cell cultures from the caudal fin of a wide range of ray-finned fishes including marine and freshwater species. The proliferative potential of these cultures makes them useful for several applications and the minimally invasive feature of this protocol makes it suitable for use in conservation plans.

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Section: Establishment Of Continuous Ray-finned Fish Cell Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Universal conditions for establish continuous cell cultures in ray-finned fishes

Cardoso,

Nascimento Oliveira,

Climaco

et al. 2023

Preprint

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show abstract

“…Such infections are characterized by severe clinical signs, such as lethargy, ocular abnormalities, and skin erosion [ 5 , 9 ]. While a variety of diagnostic methods have been developed to detect TiLV, including molecular assays [ 13 ], cell culture remains the gold standard for virus isolation [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

A New Cell Line from the Brain of Red Hybrid Tilapia (Oreochromis spp.) for Tilapia Lake Virus Propagation

Mohamad,

Khemthong,

Trongwongsa

et al. 2024

Animals

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Tilapia lake virus (TiLV) presents a substantial threat to global tilapia production. Despite the development of numerous cell lines for TiLV isolation and propagation, none have been specifically derived from red hybrid tilapia (Oreochromis spp.). In this study, we successfully established a new cell line, RHTiB, from the red hybrid tilapia brain. RHTiB cells were cultured for 1.5 years through over 50 passages and demonstrated optimal growth at 25 °C in Leibovitz-15 medium supplemented with 10% fetal bovine serum at pH 7.4. Morphologically, RHTiB cells displayed a fibroblast-like appearance, and cytochrome oxidase I gene sequencing confirmed their origin from Oreochromis spp. Mycoplasma contamination testing yielded negative results. The revival rate of the cells post-cryopreservation was observed to be between 75 and 80% after 30 days. Chromosomal analysis at the 25th passage revealed a diploid count of 22 pairs (2n = 44). While no visible cytopathic effects were observed, both immunofluorescence microscopy and RT-qPCR analysis demonstrated successful TiLV propagation in the RHTiB cell line, with a maximum TiLV concentration of 107.82 ± 0.22 viral copies/400 ng cDNA after 9 days of incubation. The establishment of this species-specific cell line represents a valuable advancement in the diagnostic and isolation tools for viral diseases potentially impacting red hybrid tilapia.

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Development of cell lines from brain, spleen and heart of ornamental blood parrot cichlid and their susceptibility to Tilapia tilapinevirus

et al. 2022

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Comparative sensitivity of three new cell lines developed from gill, liver and brain tissues of goldfish, Carassius auratus (L.) to cyprinid herpesvirus-2 (CyHV-2)

Cited by 5 publications

References 28 publications

Universal conditions for establish continuous cell cultures in ray-finned fishes

Universal conditions for establish continuous cell cultures in ray-finned fishes

A New Cell Line from the Brain of Red Hybrid Tilapia (Oreochromis spp.) for Tilapia Lake Virus Propagation

Development of cell lines from brain, spleen and heart of ornamental blood parrot cichlid and their susceptibility to Tilapia tilapinevirus

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