Comparative sequence analysis of the catB gene from Clostridium butyricum

Huggins, Andrea S.; Bannam, Trudi Leanne; Rood, Julian I.

doi:10.1128/aac.36.11.2548

Cited by 16 publications

(11 citation statements)

References 31 publications

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“…Until recently, all chloramphenicol acetyltransferases (CATs) that had been examined were members of the same family of proteins [1,2]. For simplicity, members of this family are referred to as CATA proteins and the genes as catA.…”

Section: Introductionmentioning

confidence: 99%

Characterisation of a chloramphenicol acetyltransferase determinant found in the chromosome of Pseudomonas aeruginosa

White

1999

FEMS Microbiology Letters

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The open reading frame (ORF) in the Pseudomonas aeruginosa chromosome, whose product resembles the chloramphenicol acetyltransferases (CAT) belonging to the CATB family, was cloned and shown to confer resistance to chloramphenicol (Cm) in Escherichia coli. The determinant was therefore named catB7 and the corresponding protein CATB7. When the copy number and expression signals were identical, the catB7 gene conferred resistance to Cm at a level slightly lower than those of three other catB genes. CATB7 resembles other CATBs in that it acetylates Cm but not 1-acetoxy-Cm. For CATB7, the K m values for acetyl-CoA and Cm were 5.0^5.4-fold higher than the corresponding values for each of the three other CATB proteins (CATB1, CATB3 and CATB5) examined and the V mx was 5^6 fold lower. Using PCR, the catB7 gene was found in all six P. aeruginosa strains examined but not in any other species of pseudomonad tested. Weak CAT activity was detected in crude cell extracts from five of the six P. aeruginosa strains. However, this activity did not correlate with the Cm susceptibility of the strains, indicating that catB7 is not likely to be the major determinant of intrinsic Cm resistance in P. aeruginosa. z 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

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Section: Introductionmentioning

confidence: 99%

Characterisation of a chloramphenicol acetyltransferase determinant found in the chromosome of Pseudomonas aeruginosa

White

1999

FEMS Microbiology Letters

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“…Comparison of the amino acid sequences of 17 CAT proteins from gram-negative and gram-positive bacteria has revealed a significant degree of homology between the various enzymes, and their phylogenetic relationship has been established (2,18,29). Nucleotide sequences are now available for 13 cat genes originating in gram-positive bacteria.…”

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“…Nucleotide sequences are now available for 13 cat genes originating in gram-positive bacteria. These are the gene cat-86 from Bacillus pumilus (10); those carried by the staphylococcal plasmids pC194 (11), pC221 (32), pSCS1 (30), pSCS5 (28), pSCS6 (7), pSCS7 (29), and pUB112 (5) and by the streptococcal plasmid pIP501 (12,35); and the four clostridial genes catB (18), catD (37), catP (34), and catQ (2). On the basis of these sequence data and on DNA-DNA hybridizations under stringent conditions, a minimum of seven distinct classes of cat determinants could be defined: cat-86; catpC194; catpIP501' catpC221, catpUB112 cat SCS1, and catpSCS6; catpC223, catpSC55, and catpSCS7; cati) and catP; catQ; and catB.…”

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Study of heterogeneity of chloramphenicol acetyltransferase (CAT) genes in streptococci and enterococci by polymerase chain reaction: characterization of a new CAT determinant

Trieu-Cuot

Cespédès

Bentorcha

et al. 1993

Antimicrob Agents Chemother

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An assay based on the utilization of degenerate primers that enable enzymatic amplification of an internal fragment of cat genes known to be present in gram-positive cocci was developed to identify the genes encoding chloramphenicol resistance in streptococci and enterococci. The functionality of this system was illustrated by the detection of cat genes belonging to four different hydridization classes represented by the staphylococcal genes catpC221, catpC1949 catpSCS79 and the clostridial gene catP, and by the characterization of a new streptococcal cat gene designated catS. A sequence related to the clostridial catQ gene, which was present in one streptococcal strain, was not detected by this assay. These results reveal that these six cat genes account for chromosomal-borne chloramphenicol resistance in 12 group A, B, and G streptococci tested. By contrast, only three of these six cat genes (catp'221, catj%94 and catpsCS7) were detected on the 10 enterococcal plasmids studied here that encode resistance to chloramphenicol.Chloramphenicol resistance (Cmr) in bacteria of clinical importance is generally due to the synthesis of the enzyme chloramphenicol acetyltransferase (CAT) which inactivates the antibiotic by converting it successively to 3-acetyl and 1,3-diacetyl derivatives (31). Comparison of the amino acid sequences of 17 CAT proteins from gram-negative and gram-positive bacteria has revealed a significant degree of homology between the various enzymes, and their phylogenetic relationship has been established (2, 18, 29). Nucleotide sequences are now available for 13 cat genes originating in gram-positive bacteria. These are the gene cat-86 from Bacillus pumilus (10); those carried by the staphylococcal plasmids pC194 (11), pC221 (32), pSCS1 (30), pSCS5 (28), pSCS6 (7), pSCS7 (29), and pUB112 (5) and by the streptococcal plasmid pIP501 (12,35) Utilization of degenerate primers in polymerase chain reaction (PCR) enables the detection of rRNA methylase genes that confer resistance to macrolide-lincosamide-streptogramin B-type (MLS) antibiotics in gram-positive bacteria (1). We have developed a similar assay in order to characterize the cat genes present in the remaining seven plasmidfree streptococci (23) and in the three Enterococcus faecalis plasmids (26) which did not detectably hybridize with catp.221 (or catpIP501) and cat C194* In addition, one E. faecalis and six Enterococcus [aecium plasmids coding for Cmr were included in this study. MATERIALS AND METHODSBacterial strains and culture conditions. The main characteristics of the strains and plasmids used in this study which carried unidentified cat genes are listed in Table 1

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“…Research in this laboratory has involved the cloning and molecular analysis of antibiotic resistance determinants from the gram-positive pathogen Clostridium perfringens (1,2,4,15,27,30). As part of these studies, an MLS resistance determinant, ermBP, from C. perfringens CP592 was cloned and shown to belong to the ErmB-ErmAM hybridization class (5,29).…”

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Cloning and sequence analysis of ermQ, the predominant macrolide-lincosamide-streptogramin B resistance gene in Clostridium perfringens

Berryman

Lyristis

Rood

1994

Antimicrob Agents Chemother

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The erythromycin resistance determinant from Clostridium perfringens JIR100 has been cloned, sequenced, and shown to be expressed in Escherichia coli. An open reading frame with sequence similarity to erm genes from other bacteria was identified and designated the ermQ gene. On the basis of comparative sequence analysis, it was concluded that the ermQ gene represented a new Erm hybridization class, designated ErmQ. Genes belonging to the ErmQ class were found to be widespread in C. perfringens, since 30 of 38 macrolide-lincosamide-streptogramin B-resistant C. perfringens strains, from diverse sources, hybridized to an ermQ-specific gene probe. The ermQ gene therefore represents the most common erythromycin resistance determinant in C. perfringens.

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Comparative sequence analysis of the catB gene from Clostridium butyricum

Cited by 16 publications

References 31 publications

Characterisation of a chloramphenicol acetyltransferase determinant found in the chromosome of Pseudomonas aeruginosa

Characterisation of a chloramphenicol acetyltransferase determinant found in the chromosome of Pseudomonas aeruginosa

Study of heterogeneity of chloramphenicol acetyltransferase (CAT) genes in streptococci and enterococci by polymerase chain reaction: characterization of a new CAT determinant

Cloning and sequence analysis of ermQ, the predominant macrolide-lincosamide-streptogramin B resistance gene in Clostridium perfringens

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