Comparative sequence and expression analysis of tapetum specific male sterility related genes in Medicago truncatula

Abstract

ABSTRACT. Heterosis, or enhancement through outbreeding, is one of the most promising approaches for increasing crop yield. Male sterility (MS), which promotes heterosis, has been widely applied in hybrid crop production. Medicago truncatula is a model legume species and is closely related to M. sativa, an important legume forage plant. Although the molecular mechanisms of MS in M. truncatula and M. sativa remain unclear, several studies of MS have been conducted in Arabidopsis thaliana. Previous research has shown that MS is associated with the destruction of tapetal cell layers. Disruption of tapetum developmental processes may result in pollen abortion. In an effort to identify genes useful for breeding in M. sativa, we identified MS related genes in M. truncatula using BLAST and homology to A. thaliana genes. In this study, we identified 63 tapetum specific male sterility (TSMS) related genes. The length of TSMS genes varied from 225 to 3747 bp. We identified 15 conserved domains and 8 cis-elements associated with TSMS related genes. Analysis of the phylogenetic relationships among these genes allowed them to be classified into three groups, MtTsms A, MtTsms B, and MtTsms C. Expression analyses revealed that these genes may be involved in developmental processes and response to abiotic stress.

Heat shock protein 60 expression and localisation in different tissues and testis development of male cattle (cattle-yak and yak)

“…The level of expression of HSPs throughout the period of heat stress was highest in buffaloes, followed by bos indicus and taurus [9]. In addition, the HSPA2 expression levels significantly differed in the various tissues of yak, especially in the testes and breast, followed by the brain, kidney, heart, lung, and liver, and its weakest expression was found in the spleen [10]. In relation to these backdrops, this study was performed to investigate whether Hsp60 was differentially expressed in the various tissues of cattle-yak and yak.…”

“…These factors include testicular dysplasia, hindered spermatogenesis, metamorphosis phase, and sustentacular cells assisting the germ cell differentiation in the seminiferous tubule [14]. Thus, studies on the fertility of male cattle-yak and male yak have focused on distinguishing histologic structural development, the effects of genes expression , and the intensity of protein involved in the production and control of sperms [15].…”

Transcriptome profiling of differentially expressed genes in cytoplasmic male-sterile line and its fertility restorer line in pigeon pea (Cajanus cajan L.)

“…In flowering plants, anther development is a highly controlled mechanism which demands proper differentiation of the sporogenous tissue and timely programmed microsporogenesis [11]. The absence of male gametophyte (stamen) or defective development of anther, disruption of the pollen mother cells (PMC) or tapetum cells, abnormal development of pollen, and failure of anther dehiscence leads to pollen abortion [12,13]. In all cases, CMS due to the dysfunctional mitochondrial genome is mainly responsible for the disruption of pollen development [14].…”

Regulation by HSP70/90 in the different tissues and testis development of male cattle (Cattle-yak and Yak)

“…These factors include testicular dysplasia, hindered spermatogenesis, metamorphosis phase, and sustentacular cells assisting the germ cell differentiation in the seminiferous tubule (Lu and Zi 2014). Thus, studies on the fertility of male cattle-yak and male yak have focused on distinguishing histologic structural development, the effect of gene expression genes, and the intensity of protein involved in the production and control of sperms (Shao et al 2016).…”