Comparative spatial lipidomics analysis reveals cellular lipid remodelling in different developmental zones of barley roots in response to salinity

Abstract

Salinity‐induced metabolic, ionic, and transcript modifications in plants have routinely been studied using whole plant tissues, which do not provide information on spatial tissue responses. The aim of this study was to assess the changes in the lipid profiles in a spatial manner and to quantify the changes in the elemental composition in roots of seedlings of four barley cultivars before and after a short‐term salt stress. We used a combination of liquid chromatography–tandem mass spectrometry, inductively coupled plasma mass spectrometry, matrix‐assisted laser desorption/ionization mass spectrometry imaging, and reverse transcription – quantitative real time polymerase chain reaction platforms to examine the molecular signatures of lipids, ions, and transcripts in three anatomically different seminal root tissues before and after salt stress. We found significant changes to the levels of major lipid classes including a decrease in the levels of lysoglycerophospholipids, ceramides, and hexosylceramides and an increase in the levels of glycerophospholipids, hydroxylated ceramides, and hexosylceramides. Our results revealed that modifications to lipid and transcript profiles in plant roots in response to a short‐term salt stress may involve recycling of major lipid species, such as phosphatidylcholine, via resynthesis from glycerophosphocholine.

Overexpression of phosphatidylserine synthase IbPSS1 affords cellular Na+ homeostasis and salt tolerance by activating plasma membrane Na+/H+ antiport activity in sweet potato roots

“…Although we did not observe salt-induced transcriptional changes in IbPSS1 in sweet potato roots (Fig. 2c ), high-throughput lipidomic profiling has proven that the PS abundance in different plant tissues changes in response to various environmental stresses 25 , 39 , 40 .…”

“…2c), high-throughput lipidomic profiling has proven For all the histograms, the columns labeled with "*," "**," and "***" indicate significant differences at P < 0.05, P < 0.01, and P < 0.001, respectively. that the PS abundance in different plant tissues changes in response to various environmental stresses 25,39,40 .…”

Insights Into Oxidized Lipid Modification in Barley Roots as an Adaptation Mechanism to Salinity Stress

“…Recent studies on two barley varieties (Clipper vs. Sahara) with contrasting response to salt stress have displayed a diversity of lipid alterations using lipidomics approaches (Natera et al, 2016). Spatial lipidomics based on imaging mass spectrometry (IMS) has also revealed differential lipid profiles under salt stress in barley roots and seeds (Sarabia et al, 2018;Gupta et al, 2019;Sarabia et al, 2019) The investigation of lipid profiles across different genotypes with contrasting salinity tolerance levels will lead to a better understanding of existing genetic diversity in current crop germplasm and shed more light into so-far unexplored functions of lipids in salinity tolerance. Our results deliver new insights into the impact of salt stress on the oxidized membrane lipids of barley roots and possible mechanisms of salt adaption of cereal crops.…”

Integrative Analysis of Lipidomics and Transcriptomics Revealed Dynamic Details of Lipids Metabolism and Accumulation in Developing Tiger Nut&nbsp;(Cyperus Esculentus) Tubers

“…Over the last decades, ESI-MS usage has experienced considerable improvements in plant biotechnology, accelerating the research rhythmic on lipidomics as well. Similar techniques have been applied to descript regulation of lipid modeling to various plant tissues [3,4,5]and such studies have been conducted on several plants. Lipidomics was also used to restore lipid modeling species under varied growth conditions, providing insights into the biochemical features of lipid metabolism during plant tissue's development.…”