Comparative spatial spread overtime of Zucchini Yellow Mosaic Virus (ZYMV) and Watermelon Mosaic Virus (WMV) in fields of transgenic squash expressing the coat protein genes of ZYMV and WMV, and in fields of nontransgenic squash

Klas, Ferdinand E.; Fuchs, Marc

doi:10.1007/s11248-006-9001-y

Cited by 28 publications

(13 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Resistance observed in transgenic tobacco plants with NIa or NIa-NIb-CP sequences of Potato virus Y (79) could be due to the involvement of NIa-Pro and CP as effectors of SIE. Similarly, resistance of CPbased transgenic plants against Papaya ring spot virus, Zucchini yellow mosaic virus, and Watermelon mosaic virus (80,81) could at least partially be due to CP as an effector of SIE. However, the RNA transcripts of these transgenes themselves also may have provided some degree of resistance.…”

Section: Discussionmentioning

confidence: 99%

The Coat Protein and NIa Protease of Two Potyviridae Family Members Independently Confer Superinfection Exclusion

Satyanarayana

French

2016

J Virol

View full text Add to dashboard Cite

Superinfection exclusion (SIE) is an antagonistic virus-virus interaction whereby initial infection by one virus prevents subsequent infection by closely related viruses. Although SIE has been described in diverse viruses infecting plants, humans, and animals, its mechanisms, including involvement of specific viral determinants, are just beginning to be elucidated. In this study, SIE determinants encoded by two economically important wheat viruses, Wheat streak mosaic virus (WSMV; genus Tritimovirus, family Potyviridae) and Triticum mosaic virus (TriMV; genus Poacevirus, family Potyviridae), were identified in gain-of-function experiments that used heterologous viruses to express individual virus-encoded proteins in wheat. Development of reverse genetics systems for viruses has revolutionized the understanding of viral replication, infection, and disease development through identification of viral determinants involved in these processes as well as the elucidation of interactions between viral and host factors (1-3). However, virusvirus interactions in hosts that facilitate superinfection exclusion (SIE) between related viruses or synergistic interactions between unrelated viruses have received less attention (4, 5). Synergistic interactions are facilitative virus-virus interactions between two or more unrelated viruses, and these interactions often cause increased virus accumulation of one or both viruses that could lead to severe disease compared to infection by individual viruses (4). In contrast, SIE is the result of antagonistic virus-virus interactions between closely related viruses (5-7).SIE, often referred to as "cross-protection" or "homologous interference," is defined as the phenomenon whereby initial infection by one virus prevents subsequent infection of preinfected cells by closely related viruses. SIE was originally observed between two strains of Tobacco mosaic virus (TMV) (6, 7), followed by observations with bacteriophages (8, 9). In TMV, cross-protection was used to examine the relatedness of newly collected virus isolates as being strains of, or distinct from, existing virus isolates (7, 10). Subsequently, cross-protection has been used for the management of plant viruses by purposefully infecting plants with mild isolates of a virus to prevent infection by severe isolates (11,12).The SIE phenomenon has been observed in diverse groups of plant-, human-, and animal-infecting viruses belonging to the reverse transcribing viruses such as Human immunodeficiency virus

show abstract

Section: Discussionmentioning

confidence: 99%

The Coat Protein and NIa Protease of Two Potyviridae Family Members Independently Confer Superinfection Exclusion

Satyanarayana

French

2016

J Virol

View full text Add to dashboard Cite

show abstract

“…However, the indirect costs due to Erwinia exposure may mitigate the reproductive benefits of the VRT. The fitness of the VRT may depend upon (a) the arrival times and transmission rate of both the target and the nontarget diseases in the population, (b) the number of cucumber beetles in the population, and (c) the proportion of plants in the population with the VRT [which will determine the increase in beetle concentration onto VRT plants as non-VRT plants become infected with virus and perhaps also affect the transmission rate of the virus (39,40)]. …”

Section: Discussionmentioning

confidence: 99%

Indirect costs of a nontarget pathogen mitigate the direct benefits of a virus-resistant transgene in wild Cucurbita

Sasu

Ferrari

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Virus-resistant transgenic squash are grown throughout the United States and much of Mexico and it is likely that the virusresistant transgene (VRT) has been introduced to wild populations repeatedly. The evolutionary fate of any resistance gene in wild populations and its environmental impacts depend upon trade-offs between the costs and benefits of the resistance gene. In a 3-year field study using a wild gourd and transgenic and nontransgenic introgressives, we measured the effects of the transgene on fitness, on herbivory by cucumber beetles, on the incidence of mosaic viruses, and on the incidence of bacterial wilt disease (a fatal disease vectored by cucumber beetles). In each year, the first incidence of zucchini yellow mosaic virus occurred in mid-July and spread rapidly through the susceptible plants. We found that the transgenic plants had greater reproduction through both male and female function than the susceptible plants, indicating that the VRT has a direct fitness benefit for wild gourds under the conditions of our study. Moreover, the VRT had no effect on resistance to cucumber beetles or the incidence of wilt disease before the spread of the virus. However, as the virus spread through the fields, the cucumber beetles became increasingly concentrated upon the healthy (mostly transgenic) plants, which increased exposure to and the incidence of wilt disease on the transgenic plants. This indirect cost of the VRT (mediated by a nontarget herbivore and pathogen) mitigated the overall beneficial effect of the VRT on fitness.Cucurbita pepo ͉ Erwinia tracheiphyla ͉ plant-herbivore-pathogen interaction ͉ zucchini yellow mosaic virus ͉ cucumber beetles

show abstract

“…Post‐transcriptional gene silencing (PTGS) and RNA interference (RNAi) are technologies that offer significant potential to control plant viral pathogens, particularly RNA viruses. RNAi has been applied to generate resistance to Cucumber mosaic virus , Zucchini yellow mosaic virus and Watermelon mosaic virus 2 (Klas et al ., 2006; Tricoll et al ., 1995), Potato leaf roll virus , Potato virus Y and Potato virus X (Thomas et al ., 2000), Papaya ring spot virus (Krubphachaya et al ., 2007) and Plum pox virus (Hily et al ., 2004; Kundu et al ., 2008).…”

Section: Introductionmentioning

confidence: 99%

RNAi‐mediated resistance to Cassava brown streak Uganda virus in transgenic cassava

Yadav¹,

Ogwok²,

Wagaba³

et al. 2011

Molecular Plant Pathology

View full text Add to dashboard Cite

Cassava brown streak disease (CBSD), caused by Cassava brown streak Uganda virus (CBSUV) and Cassava brown streak virus (CBSV), is of new epidemic importance to cassava (Manihot esculenta Crantz) production in East Africa, and an emerging threat to the crop in Central and West Africa. This study demonstrates that at least one of these two ipomoviruses, CBSUV, can be efficiently controlled using RNA interference (RNAi) technology in cassava. An RNAi construct targeting the near full-length coat protein (FL-CP) of CBSUV was expressed constitutively as a hairpin construct in cassava. Transgenic cassava lines expressing small interfering RNAs (siRNAs) against this sequence showed 100% resistance to CBSUV across replicated graft inoculation experiments. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis showed the presence of CBSUV in leaves and some tuberous roots from challenged controls, but not in the same tissues from transgenic plants. This is the first demonstration of RNAi-mediated resistance to the ipomovirus CBSUV in cassava.

show abstract

Comparative spatial spread overtime of Zucchini Yellow Mosaic Virus (ZYMV) and Watermelon Mosaic Virus (WMV) in fields of transgenic squash expressing the coat protein genes of ZYMV and WMV, and in fields of nontransgenic squash

Cited by 28 publications

References 28 publications

The Coat Protein and NIa Protease of Two Potyviridae Family Members Independently Confer Superinfection Exclusion

The Coat Protein and NIa Protease of Two Potyviridae Family Members Independently Confer Superinfection Exclusion

Indirect costs of a nontarget pathogen mitigate the direct benefits of a virus-resistant transgene in wild Cucurbita

RNAi‐mediated resistance to Cassava brown streak Uganda virus in transgenic cassava

Contact Info

Product

Resources

About