Comparative Spectrophotometric, Spectrofluorometric, and High‐performance Liquid Chromatographic Study for the Quantitative Determination of the Binary Mixture Felodipine and Ramipril in Pharmaceutical Formulations

Yazbi, Fawzy A. El; Mahrous, Mohamad E.; Hammud, Hassan H.; Sonji, Ghassan; Sonji, Nada

doi:10.1080/00032710801934643

Cited by 11 publications

(7 citation statements)

References 19 publications

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“…and ). The spectra of the studied drugs in each mixture are completely overlapped and they have very close excitation and emission maxima (El Yazbi et al ., ; Walash et al ., ); therefore, it is difficult to distinguish between them using the traditional spectrophotometric or spectrofluorimetric methods. In the current work, complete resolution was obtained and each member of the studied drugs can be distinguished with its specified R f value.…”

Section: Resultsmentioning

confidence: 99%

“…In recent years, FLD and RMP have been successfully used in combination in the treatment of hypertension, and pharmaceutical preparations containing both drugs have been marketed, permitting once‐a‐day administration. Although many methods have been reported in the literature for the determination of FLD and RMP individually or in other combinations, there are few spectrophotometric, spectrofluorimetric and RP‐HPLC methods for the determination of an FLD–RMP combined dosage (El Yazbi et al ., ; Rontogianni et al ., ).…”

Section: Introductionmentioning

confidence: 99%

“…Individual determination of FLD has been carried out in human plasma by capillary gas chromatography (Ahnoff, ), high selectivity gas chromatography (Ahnoff et al ., ) and HPLC (Lindmark et al ., ; Lopez et al ., ; Soons et al ., ; Wen‐Gang et al ., ). In pharmaceutical formulations, FLD has been determined by HPLC (Baranda et al ., ; Björklund et al ., ; Cardoza and Amin, ; El Yazbi et al ., ; Fusun et al ., ; Karlsson et al ., ; Salem and Abdallah, ), spectrofluorimetric (El Yazbi et al ., ; Walash et al ., ) and spectrophotometric methods (Basavaiah et al ., ; El Yazbi et al ., ; Fusun et al ., ; Gottfries et al ., ; Marciniec et al ., ; Meyyanathan et al ., ; Salem and Abdallah, ).…”

Section: Introductionmentioning

confidence: 99%

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Development and validation of TLC–densitometric method for simultaneous determination of two binary antihypertensive mixtures containing felodipine in fixed dose combinations

Mohamed

Omar

Hammad

et al. 2015

Biomedical Chromatography

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A new densitometric thin-layer chromatographic method has been developed for simultaneous determination of two binary mixtures containing felodipine in combination with either metoprolol (mixture I) or ramipril (mixture II). The two mixtures were quantitatively separated on 60 F254 silica gel plates using toluene-ethyl acetate-methanol-ammonia as mobile phase with UV detection at 233 and 229 nm for mixtures I and II, respectively. The studied drugs were satisfactorily resolved with retention factor (Rf ) values of 0.34 ± 0.03 and 0.65 ± 0.03 for metoprolol and felodipine, respectively, in mixture I and 0.35 ± 0.03 and 0.74 ± 0.03 for ramipril and felodipine, respectively, in mixture II. Linearity ranges were 2000-7000 and 200-700 ng/band for metoprolol and felodipine, respectively, in mixture I and 1500-4000 ng/band for both ramipril and felodipine in mixture II. Correlation coefficient (r) values were 0.9968 for both metoprolol and felodipine in mixture I and 0.9993 for ramipril and 0.9989 for felodipine in mixture II. The method has been validated according to International Conference on Harmonization guidelines and has been successfully applied for determination of the studied drugs in their dosage forms without interference from commonly encountered excipients.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development and validation of TLC–densitometric method for simultaneous determination of two binary antihypertensive mixtures containing felodipine in fixed dose combinations

Mohamed

Omar

Hammad

et al. 2015

Biomedical Chromatography

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“…(3) Graphical Method . To avoid the excessive preparation of several standard solutions of either compound Carmoisine or in order to locate the exact balance point, a graphical method is recommended [ 19 , 20 ].…”

Section: Methodsmentioning

confidence: 99%

Four Derivative Spectrophotometric Methods for the Simultaneous Determination of Carmoisine and Ponceau 4R in Drinks and Comparison with High Performance Liquid Chromatography

Turak

Dinç²,

Dülger

et al. 2014

International Journal of Analytical Chemistry

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Four simple, rapid, and accurate spectrophotometric methods were developed for the simultaneous determination of two food colorants, Carmoisine (E122) and Ponceau 4R (E124), in their binary mixtures and soft drinks. The first method is based on recording the first derivative curves and determining each component using the zero-crossing technique. The second method uses the first derivative of ratio spectra. The ratio spectra are obtained by dividing the absorption spectra of the binary mixture by that of one of the components. The third method, derivative differential procedure, is based on the measurement of difference absorptivities derivatized in first order of solution of drink samples in 0,1 N NaOH relative to that of an equimolar solution in 0,1 N HCl at wavelengths of 366 and 451 nm for Carmoisine and Ponceau 4R, respectively. The last method, based on the compensation method is presented for derivative spectrophotometric determination of E122 and E124 mixtures with overlapping spectra. By using ratios of the derivative maxima, the exact compensation of either component in the mixture can be achieved, followed by its determination. These proposed methods have been successfully applied to the binary mixtures and soft drinks and the results were statistically compared with the reference HPLC method (NMKL 130).

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“…Regarding FDP, a convential fluorimetric method was reported for its determination in presence of ramipril in their combined tablets Triacor ® [7]. …”

Section: Introductionmentioning

confidence: 99%

Synchronous fluorescence spectrofluorimetric method for the simultaneous determination of metoprolol and felodipine in combined pharmaceutical preparation

Walash

Belal

El‐Enany

et al. 2011

Chemistry Central Journal

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A rapid, simple and sensitive synchronous specrtofluorimetric method has been developed for the simultaneous analysis of binary mixture of metoprolol (MTP) and felodipine (FDP). The method is based upon measurement of the synchronous fluorescence intensity of the two drugs at Δλ of 70 nm in aqueous solution. The different experimental parameters affecting the synchronous fluorescence intensities of the two drugs were carefully studied and optimized. The fluorescence intensity-concentration plots were rectilinear over the ranges of 0.5-10 μg/mL and 0.2-2 μg/mL for MTP and FDP, respectively. The limits of detection were 0.11 and 0.02 μg/mL and quantification limits were 0.32 and 0.06 μg/mL for MTP and FDP, respectively. The proposed method was successfully applied for the determination of the two compounds in their commercial tablets and the results obtained were favorably compared to those obtained with a comparison method.

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Comparative Spectrophotometric, Spectrofluorometric, and High‐performance Liquid Chromatographic Study for the Quantitative Determination of the Binary Mixture Felodipine and Ramipril in Pharmaceutical Formulations

Cited by 11 publications

References 19 publications

Development and validation of TLC–densitometric method for simultaneous determination of two binary antihypertensive mixtures containing felodipine in fixed dose combinations

Development and validation of TLC–densitometric method for simultaneous determination of two binary antihypertensive mixtures containing felodipine in fixed dose combinations

Four Derivative Spectrophotometric Methods for the Simultaneous Determination of Carmoisine and Ponceau 4R in Drinks and Comparison with High Performance Liquid Chromatography

Synchronous fluorescence spectrofluorimetric method for the simultaneous determination of metoprolol and felodipine in combined pharmaceutical preparation

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