1995
DOI: 10.1007/bf00199344
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Comparative studies of collagenase and stromelysin-1 expression by rheumatoid synoviocytes in vitro

Abstract: Matrix metalloproteinases such as collagenase and stromelysin are recognised as important cartilage-degrading enzymes in the pathophysiology of rheumatoid arthritis. Synovial fibroblasts and macrophages are the major cellular components of rheumatoid synovium, but the regulation and relative expression of collagenase and stromelysin by these two cell types remains uncertain. Using in vitro cultures of adherent rheumatoid synovial cells we have examined the coordinate or separate expression of collagenase and s… Show more

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Cited by 17 publications
(16 citation statements)
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“…The hyperplastic rheumatoid lining layer contains a preponderance of macrophages (30), which secrete both IL-1 and tumor necrosis factor ␣ (TNF␣), the cytokines that drive the inflammatory and destructive processes in RA (31). Macrophages also produce proteolytic enzymes (32,33), although fibroblasts, stimulated by macrophagederived products, are the major source of proteases.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…The hyperplastic rheumatoid lining layer contains a preponderance of macrophages (30), which secrete both IL-1 and tumor necrosis factor ␣ (TNF␣), the cytokines that drive the inflammatory and destructive processes in RA (31). Macrophages also produce proteolytic enzymes (32,33), although fibroblasts, stimulated by macrophagederived products, are the major source of proteases.…”
Section: Discussionmentioning
confidence: 99%
“…However, the correlation between lining layer expression of CB and CL did not reach statistical significance. This may reflect the fact that CD68Ϫ synovial fibroblasts are also a major source of proteases (33). Furthermore, the CD68 surface marker denotes macrophage maturation (38), and it is possible that different proteases are produced at different stages of macrophage development.…”
Section: Discussionmentioning
confidence: 99%
“…Collagenase 1 was measured using an ELISA assay based on methodology described previously 26. The assay used a monospecific rabbit polyclonal anti-human collagenase 1 as the capture antibody and a monospecific biotinylated sheep anti-collagenase 1 antibody for antigen detection.…”
Section: Methodsmentioning
confidence: 99%
“…Stromelysin 1 was assayed using a double antibody ELISA system developed within the laboratory 26. The 96 well plates were coated with a monospecific rabbit polyclonal anti-human stromelysin 1 antibody as the capture antibody, with a monospecific biotinylated sheep polyclonal anti-stromelysin 1 antibody for antigen detection.…”
Section: Methodsmentioning
confidence: 99%
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