Comparative Studies of Diagnostic Bacteriological Methods for the Recovery of <i>Salmonella</i> from Faecal Samples from Flocks of Layers

Braun, Carla Luciane Kreutz; Kostka, V.; Balks, Elisabeth; Redmann, Thomas; Helmuth, Reiner

doi:10.1111/j.1439-0450.1998.tb00789.x

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…Only overnight incubation was used for pre‐enrichment and enrichment, and it is possible that more positive flocks would have been found if other procedures had been used. It has been reported that further enrichment steps or delayed secondary enrichment may result in more positive samples (Braun and others 1998, Davies and others 2000). However, preliminary comparative studies in the authors' laboratory have revealed only marginal differences (J. C. Jörgensen, unpublished observations).…”

Section: Discussionmentioning

confidence: 99%

Serovars of Salmonellaisolated from Danish turkeys between 1995 and 2000 and their antimicrobial resistance

Pedersen¹,

Hansen²,

Jørgensen³

et al. 2002

Veterinary Record

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The prevalence of Salmonella serovars and their antimicrobial resistance patterns were investigated among Danish turkeys between 1995 and 2000, by sampling the flocks approximately 14 days before they were slaughtered. Within the flocks, the prevalence of salmonella varied from 7.1 per cent to 25 per cent, and 24 different serovars were detected. The five most prevalent, which accounted for 58.5 per cent of the isolates were Salmonella Heidelberg (16.2 per cent of the isolates), Salmonella Agona (15.8 per cent), Salmonella Derby (12.4 per cent), Salmonella Muenster (7.3 per cent) and Salmonella Anatum (6.8 per cent). In addition, a few rough isolates and isolates belonging to the antigenically incomplete formulae 6,7:-:- and 4,12:b:- were found. The level of antimicrobial resistance was low; the highest resistance was recorded to ampicillin (13.7 per cent) and streptomycin (9.0 per cent) followed by tetracycline (8.5 per cent), sulphonamides (7.7 per cent) and spectinomycin (4.7 per cent). Resistance to quinolones was very low: four isolates were resistant to nalidixic acid, and only one was resistant to enrofloxacin. No resistance was recorded to colistin, apramycin, ceftiofur, florfenicol, or amoxycillin with clavulanic acid. Only 24 isolates were resistant to two or more compounds in various combinations of up to six compounds; one Salmonella Havana isolate was resistant to six compounds. Six isolates were serovar Typhimurium, but none of them belonged to phage type DT104.

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Section: Discussionmentioning

confidence: 99%

Serovars of Salmonellaisolated from Danish turkeys between 1995 and 2000 and their antimicrobial resistance

Pedersen¹,

Hansen²,

Jørgensen³

et al. 2002

Veterinary Record

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“…1992; Dusch and Altweg 1995), poultry samples (Read et al . 1994; Braun et al . 1998) and environmental samples (Read et al .…”

Section: Introductionmentioning

confidence: 99%

Comparison of selective enrichment media for the detection of Salmonella in poultry faeces

Voogt¹,

Raes²,

Wannet³

et al. 2001

Lett Appl Microbiol

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Aims: The aim of this study was to compare the results of semisolid media and Rappaport± Vassiliadis (RV) medium for the detection of Salmonella in faecal samples from broiler and layer¯ocks. Methods and Results: Three different selective enrichment media were used: (a) RV medium; (b) diagnostic semisolid Salmonella medium (DIASALM) and (c) modi®ed semisolid RV (MSRV) medium. The performance of DIASALM and MSRV was signi®cantly better compared with RV. Conclusions: The results of this study indicate that approximately 95% of the samples containing Salmonella would be detected by a combination of a semisolid medium (MSRV or DIASALM) and RV. Signi®cance and Impact of the Study: The International Standard method ISO 6579, including RV and selenite cystine broth as selective enrichment media, is most frequently used for the isolation of Salmonella from poultry faeces. This study reveals that there are more suitable combinations of selective enrichment media.

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Cultural and Immunological Detection Methods for Salmonella spp. in Animal Feeds – A Review

et al. 2006

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Food-borne salmonellosis continues to be a major public health concern, and contamination with Salmonella spp. in pre-harvest animal production is considered a primary contributor to this problem. Animal feeds can easily become contaminated during primary production, feed mixing and processing as well as during feeding. Consequently, monitoring and surveillance of feeds and feed ingredients for Salmonella spp. contamination may be useful or necessary in the prevention and control of this organism. Cultural and immunological detection methods for salmonellae have been used or suggested as possible approaches for use in animal feeds. Cultural methods remain advantageous owing to their ability to detect viable bacterial cells, while immunological methods have the capability of detecting nonculturable bacterial cells. Advancements and improvements in both methodologies offer opportunities for eventual routine use of these detection technologies in animal feed assays.

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Comparative Studies of Diagnostic Bacteriological Methods for the Recovery of Salmonella from Faecal Samples from Flocks of Layers

Cited by 3 publications

References 13 publications

Serovars of Salmonellaisolated from Danish turkeys between 1995 and 2000 and their antimicrobial resistance

Serovars of Salmonellaisolated from Danish turkeys between 1995 and 2000 and their antimicrobial resistance

Comparison of selective enrichment media for the detection of Salmonella in poultry faeces

Cultural and Immunological Detection Methods for Salmonella spp. in Animal Feeds – A Review

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