Comparative studies of mesenchymal stem cells derived from different cord tissue compartments – The influence of cryopreservation and growth media

Dulugiac, Magda; Moldovan, Lucia; Zarnescu, Otilia

doi:10.1016/j.placenta.2015.08.011

Cited by 16 publications

(8 citation statements)

References 33 publications

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“…Cryopreservation is currently the best way for long‐term cell storage; MSCs, isolated by explant method, can be successfully revitalized after cryopreservation while maintaining their characteristics . In addition, after cryopreservation of UC tissue, it is possible to isolate MSCs by explant method from post‐thaw tissue pieces …”

Section: Comparison Of Mscs Isolated By Enzymatic and Explant Methodsmentioning

confidence: 99%

Explant culture: An advantageous method for isolation of mesenchymal stem cells from human tissues

Hendijani

2017

Cell Proliferation

146

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Mesenchymal stem cell (MSC) research progressively moves towards clinical phases. Accordingly, a wide range of different procedures were presented in the literature for MSC isolation from human tissues; however, there is not yet any close focus on the details to offer precise information for best method selection. Choosing a proper isolation method is a critical step in obtaining cells with optimal quality and yield in companion with clinical and economical considerations. In this concern, current review widely discusses advantages of omitting proteolysis step in isolation process and presence of tissue pieces in primary culture of MSCs, including removal of lytic stress on cells, reduction of in vivo to in vitro transition stress for migrated/isolated cells, reduction of price, processing time and labour, removal of viral contamination risk, and addition of supporting functions of extracellular matrix and released growth factors from tissue explant. In next sections, it provides an overall report of technical highlights and molecular events of explant culture method for isolation of MSCs from human tissues including adipose tissue, bone marrow, dental pulp, hair follicle, cornea, umbilical cord and placenta. Focusing on informative collection of molecular and methodological data about explant methods can make it easy for researchers to choose an optimal method for their experiments/clinical studies and also stimulate them to investigate and optimize more efficient procedures according to clinical and economical benefits.

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Section: Comparison Of Mscs Isolated By Enzymatic and Explant Methodsmentioning

confidence: 99%

Explant culture: An advantageous method for isolation of mesenchymal stem cells from human tissues

Hendijani

2017

Cell Proliferation

146

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“…The cells were incubated at 37 °C with 5% humidified CO2, and the medium was changed every 3 days. After 14 days, the cells were stained with Oil Red O .…”

Section: Methodsmentioning

confidence: 99%

miR‐33a‐5p modulates TNF‐α‐inhibited osteogenic differentiation by targeting SATB2 expression in hBMSCs

Shi

Chen

et al. 2016

FEBS Letters

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Edited by Zhijie Chang miRNAs play a number of roles in bone, including mediating the pathological effects of inflammation. Here, we found that miR-33a-5p expression was significantly increased after TNF-a treatment during BMP-2-induced osteogenic differentiation of hBMSCs. Luciferase reporter assays and western blotting demonstrated that special AT-rich sequence-binding protein 2 (SATB2) is a target of miR-33a-5p. Moreover, we show that BMP-2 induces SATB2 expression by interacting with SATB2 directly via the BMP-2-RUNX2 pathway. However, TNF-a first decreases SATB2 expression by inhibiting miR33a-5p degradation. We thus conclude that miR-33a-5p plays a central role in this complex regulatory network. These findings will help to understand the regulatory role of miR-33a-5p in the inflammatory process.

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“…Morphological observations can be confirmed by immunofluorescence [55] or microarray investigations [62] that evidentiate specific markers expression at protein and gene level, respectively. In osteogenic differentiated DPSC, analysis of osteocalcin, osteopontin, and type I COL is performed.…”

Section: Dpsc Multidifferentiation Capacitymentioning

confidence: 86%

“…Hematoxylineosin staining of confluent DPSC revealed the fibroblast-like morphology, characteristic for the mesenchymal phenotype (Figure 2A). DPSC culture processed for transmission electron microscopy [55] exhibited typical ultrastructure of MSC with spherical or irregular shaped nucleus (N) containing a large amount of euchromatin and mitochondria-rich cytoplasm ( Figure 2B). Some cells presented rough endoplasmic reticulum (ER) with dilated cisterns (Figure 2B).…”

Section: Dpsc Propertiesmentioning

confidence: 99%

Advanced Regenerative Techniques Based on Dental Pulp Stem Cells for the Treatment of Periodontal Disease

Seciu¹,

Crăciunescu²,

Zarnescu³

2019

Periodontology and Dental Implantology

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Recent progress in periodontology intended to reduce the risk represented by periodontal disease for systemic disorders and general human health condition. In this chapter, we overview the advantages and limitations of current techniques based on occlusive membranes for periodontal regeneration. Special emphasis is paid to advanced techniques using stem cells from dental pulp for the regeneration of bone defects caused by the chronic periodontal disease. Stem cells isolation, in vitro expansion and characterization techniques are presented. Therapeutic strategies of stem cells delivery using natural polymeric carriers are discussed. Stem cell-scaffold constructs application in bone tissue engineering is proposed, taking into account the marked decline of healing, and regenerative processes in elderly individuals. Future researchers envisage multiple effects of engineered constructs with antimicrobial, anti-inflammatory, and regenerative activity for periodontal treatment.

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Comparative studies of mesenchymal stem cells derived from different cord tissue compartments – The influence of cryopreservation and growth media

Cited by 16 publications

References 33 publications

Explant culture: An advantageous method for isolation of mesenchymal stem cells from human tissues

Explant culture: An advantageous method for isolation of mesenchymal stem cells from human tissues

miR‐33a‐5p modulates TNF‐α‐inhibited osteogenic differentiation by targeting SATB2 expression in hBMSCs

Advanced Regenerative Techniques Based on Dental Pulp Stem Cells for the Treatment of Periodontal Disease

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