Comparative Studies of Ovarian Steroids in Blood, and Specific Proteolytic Enzymes in the Cervical Mucus, in Four Sheep Breeds after Oestrus Synchronization (Progesterone and PMSG). 2. Breed Variation of PAA, PAI and PI in the Cervical Mucus during Natural Oestrus, Synchronized Oestrus, and the First Oestrus after Synchronized Oestrus

Abstract

ContentsPlasminogen-activator activity (PAA), plasminogen-activator inhibition (PAI), and plasmin inhibition (PI) were studied in the cervical mucus of four sheep breeds (Friesland, Chios, Serres, Karagouniki) during natural oestrus, synchronized oestrus, and the first oestrus after synchronized oestrus.PAA had increased by the middle of natural oestrus, but with some differences among the breeds studied. Oestrous synchronization disturbed PAA in the cervical mucus, depending again on the breed. This disturbance persisted during the first oestrus after the synchronized oestrus in all breeds studied except Friesland. PAA in the cervical mucus of the ewe was due to both types of plasminogen activator (t-PA and PA1 in the cervical mucus had increased by the middle and end of natural oestrus, depending on the breed. Oestrous synchronization disturbed PAI, which was still the case even during the first oestrus after synchronized oestrus.PI had increased by the end of natural oestrus with some differences among the breeds studied. In all breeds studied, PI in the cervical mucus was disturbed during the synchronized oestrus. This disturbance of PI persisted, more or less, during the first oestrus after synchronized oestrus in all breeds studied except Friesland.Blood oestradiol-17/3, in a quite limited range of concentrations dependent on the breed and the type of the oestrus, could positively affect PAA or PA1 and negatively affect PI in the cervical mucus. U-PA) . InhaltVergleichende Untersuchungen ovarieller Steroide im Blut sowie spezifische proteolytische Enzyme im Zervikalmukus bei vier Schafrassen nach Ostrussynchronisation (Progesteron und PMSG). 2. Rassebedingte Unterschiede fur PAA, PA1 und PI im Zervikalmukus wahrend des normalen und des synchronisierten Ostrus sowie des ersten Ostrus nach der Synchronisation Plasminogen-Aktivator-Aktivitat (PAA), Plasminogen-Aktivator-Inhibition (PAI) und Plasmin-Inhibition (PI) wurden im Zervikalmukus von vier verschiedenen Schafrassen (Friesland, Chios, Serres, Karagouniki) wahrend desnormalen und synchronisierten Ostrus sowie des ersten Ostrus nach der Synchronisation untersucht. PAA-Konzentrationen stiegen in der Mitte des normalen Ostrus an. Dabei traten rassespezifische Unterschiede auf. Ebenfalls rasseabhangig war die Veranderung von PAA im Zervikalmukus bei synchronisierten Tieren. Diese Ver-anderung bestand auch im ersten Ostrus nach der Synchronisation bei allen Rassen auBer bei Friesland-Schafen. Es traten beide Typen des des Plasminogen-Aktivator (t-PA und u-PA) auf. Rasseabhangig stieg PA1 zur Mitte und am Ende des normalen Ostrus an. Ostrus-Synchronisation veranderte PAL Diese Veranderung hielt auch wahrend des auf die Synchronisation folgenden Ostrus an. PI stieg zum Ende des naturlichen Ostrus an, wobei einige Rasseunterschiede beobachtet wurden. Im synchronisierten Ostrus veranderten sich die PI-Werte im Zervikalmukus. AuBer bei Friesland-Schafen hielt diese Veranderung des PI wahrend des ersten Zyklus nach de!, Synchronisation an. Die Blutkonzentrationen vo...

Effect of Norgestomet Treatment on Plasminogen Activator Activity in the Cervical Mucus and the Endometrium in Dairy Cows

“…It is the first time that the above negative influence of progestogens on cervical mucus PAA in cow, has been estimated. In ewe, estrous synchronization with progesterone implants induced, also, a reduction in cervical mucus PAA, compared to PAA during natural oestrus (Kouskoura et al, 1995).…”

“…The synthesis and/or release of plasminogen activators in the cervical mucus could be influenced by progestogen treatment, as was found in sheep, where synchronization of oestrus using progestogens had a negative effect on cervical mucus PAA during induced oestrus (Kouskoura et al, 1995). Also, chronic progestogen treatment with oral contraceptives in women, reduce endometrial PAA (Casslen and Astedt, 1983a).…”

“…These enzymatic parameters are released during acrosome reaction (Taitzoglou et al, 1996), while PAA seems to be more potent in the ampulla of the oviduct, a region where fertilization takes place (Tsantarliotou et al, 2005). Plasminogen activators activity has been detected in the cervical mucus of the ewe, (Kouskoura et al, 1995), while an increased endometrial PAA and vascularization have been detected during implantation in various species (Fazleabas et al, 1983;Hofmann et al, 1994;Wang et al, 1996;Yamada et al, 1996).…”