Comparative studies of saxitoxin (STX) -induced cytotoxicity in Neuro-2a and RTG-2 cell lines: An explanation with respect to changes in ROS

Zhou, Zhongyuan; Tang, Xuexi; Chen, Hongmei; Wang, You

doi:10.1016/j.chemosphere.2017.10.083

Cited by 20 publications

(7 citation statements)

References 41 publications

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“…Previous studies involving 17 of the 62 strains tested here (CENA21, 67,69,135,152,153,154,159,160,185,216,217,247,271,281,283, and 510) reported anticancer and antimicrobial activity on other cell lines and microorganisms [41,42,47]. Thus, our analysis expands the number of strains producing known bioactive compounds, especially from the Nostoc and Fischerella genera [32,43,52].…”

Section: Discussionmentioning

confidence: 68%

“…Aeruginosin and anabaenopeptin are protease inhibitors with no reported cytotoxic effects. Lastly, saxitoxin is a neurotoxin that is associated with cytotoxic effects on mammalian cells [66][67][68]. Further analysis using isolated compounds would be necessary to show which are the antileukemic compounds produced by the tested Brazilian cyanobacteria.…”

Section: Discussionmentioning

confidence: 99%

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Dereplication of Natural Products with Antimicrobial and Anticancer Activity from Brazilian Cyanobacteria

Shishido

Popin

Jokela

et al. 2019

Toxins

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Cyanobacteria are photosynthetic organisms that produce a large diversity of natural products with interesting bioactivities for biotechnological and pharmaceutical applications. Cyanobacterial extracts exhibit toxicity towards other microorganisms and cancer cells and, therefore, represent a source of potentially novel natural products for drug discovery. We tested 62 cyanobacterial strains isolated from various Brazilian biomes for antileukemic and antimicrobial activities. Extracts from 39 strains induced selective apoptosis in acute myeloid leukemia (AML) cancer cell lines. Five of these extracts also exhibited antifungal and antibacterial activities. Chemical and dereplication analyses revealed the production of nine known natural products. Natural products possibly responsible for the observed bioactivities and five unknown, chemically related chlorinated compounds present only in Brazilian cyanobacteria were illustrated in a molecular network. Our results provide new information on the vast biosynthetic potential of cyanobacteria isolated from Brazilian environments.

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Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Dereplication of Natural Products with Antimicrobial and Anticancer Activity from Brazilian Cyanobacteria

Shishido

Popin

Jokela

et al. 2019

Toxins

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“…All chemicals were reagent grade or better. Analytical standards of MC-LA, MC-LR, MC-LY, MC-RR, MC-YR, NOD, anatoxin-a (ANA-a), homoanatoxin-a (HATX), dihydroanatoxin-a (H2ATX), 13 C 4 -anatoxina, CYN, 15 N 5 -CYN, SAX, and 15 N 7 -SAX were obtained from Eurofins Abraxis (Warminster, PA, USA). Isotopically labeled internal standards 15 N 7 -MC-LA, 15 N 10 -MC-LR, 15 N 13 -MC-RR, and 15 N 10 -MC-YR were obtained from Cambridge Isotope Laboratories (Tewksbury, MA, USA).…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

What, How, When, and Where: Spatiotemporal Water Quality Hazards of Cyanotoxins in Subtropical Eutrophic Reservoirs

Stroski,

Roelke,

Kieley

et al. 2024

Environ. Sci. Technol.

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Though toxins produced during harmful blooms of cyanobacteria present diverse risks to public health and the environment, surface water quality surveillance of cyanobacterial toxins is inconsistent, spatiotemporally limited, and routinely relies on ELISA kits to estimate total microcystins (MCs) in surface waters. Here, we employed liquid chromatography tandem mass spectrometry to examine common cyanotoxins, including five microcystins, three anatoxins, nodularin, cylindrospermopsin, and saxitoxin in 20 subtropical reservoirs spatially distributed across a pronounced annual rainfall gradient. Probabilistic environmental hazard analyses identified whether water quality values for cyanotoxins were exceeded and if these exceedances varied spatiotemporally. MC-LR was the most common congener detected, but it was not consistently observed with other toxins, including MC-YR, which was detected at the highest concentrations during spring with many observations above the California human recreation guideline (800 ng/L). Cylindrospermopsin was also quantitated in 40% of eutrophic reservoirs; these detections did not exceed a US Environmental Protection Agency swimming/ advisory level (15,000 ng/L). Our observations have implications for routine water quality monitoring practices, which traditionally use ELISA kits to estimate MC levels and often limit collection of surface samples during summer months near reservoir impoundments, and further indicate that spatiotemporal surveillance efforts are necessary to understand cyanotoxins risks when harmful cyanobacteria blooms occur throughout the year.

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“…That LDH released into the cell culture medium indicates the damage of the cell membrane structure (Zhou et al, 2018) and MTT assay was used to measure the cell viability. To screen out the optimal concentration of LPA on RN-SC cells, cells were incubated with increasing doses of LPA from 0 to 10 μmol/L for Figure 1A).…”

Section: The Optimum Concentration For Lpa Applied To Rn-sc Cellsmentioning

confidence: 99%

High Expression of Lysophosphatidic Acid Induces Nerve Injury in LSS Patients via AKT Mediated NF-κB p65 Pathway

Zhai¹,

Liang

2021

Front. Pharmacol.

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Lumbar spinal stenosis (LSS) is a spinal degenerative disease, complicated with nerve injury. Lysophosphatidic acid (LPA), a kind of glycerophospholipid molecule is elevated in the initial stages of neural injury. This research aimed to investigate the patho-mechanism of nerve injury caused by LPA in LSS patients. Twenty-five LSS patients and fifteen idiopathic scoliosis patients (without neurological symptoms) were recruited from Xianyang Central Hospital of Shanxi Province. We measured the concentration of LPA in cerebrospinal fluid samples of all subjects. Different concentrations (0.1, 1, and 10 mol/L) of LPA were used to stimulate Rat Neurons-spinal cord (RN-SC) cells. The effects of LPA on cell injury was detected by MTT and LDH (lactate dehydrogenase) assay. Cell apoptosis was determined by FCM (flow cytometry) and TUNEL staining. The changes in the expression of key proteins involved in Akt mediated NF-κB p65 pathway intervened by LPA were determined by western blot. RN-SC cells were pretreated with JSH-23 (NF-κB inhibitor) before LPA exposure, followed by cell apoptosis measurement. The concentration of LPA in LSS patients was notably higher than that in control patients (p < 0.01). The level of LPA was positively correlated with the severity of LSS. LPA treatment induced RN-SC cells displaying oval or rounded cell body with degenerated protrusion dose dependently. In addition, LPA decreased RN-SC cell viability and promoted cell apoptosis in a dose-dependent manner. LPA initiated Akt phosphorylation, IKB phosphorylation, and NF-κB nuclear translocation in a dose-dependent manner. However, JSH-23 (NF-κB inhibitor) pre-treatment prevented effects of LPA. The high levels of LPA induced nerve injury by reducing the viability of RN-SC cells and promoted cell apoptosis through Akt mediated NF-κB p65 signaling pathway. LPA might be a new therapeutic target for relieving nerve injury in LSS patients.

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Comparative studies of saxitoxin (STX) -induced cytotoxicity in Neuro-2a and RTG-2 cell lines: An explanation with respect to changes in ROS

Cited by 20 publications

References 41 publications

Dereplication of Natural Products with Antimicrobial and Anticancer Activity from Brazilian Cyanobacteria

Dereplication of Natural Products with Antimicrobial and Anticancer Activity from Brazilian Cyanobacteria

What, How, When, and Where: Spatiotemporal Water Quality Hazards of Cyanotoxins in Subtropical Eutrophic Reservoirs

High Expression of Lysophosphatidic Acid Induces Nerve Injury in LSS Patients via AKT Mediated NF-κB p65 Pathway

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