Comparative Studies of Selected Criteria Enabling Optimization of the Extraction of Polar Biologically Active Compounds from Alfalfa with Supercritical Carbon Dioxide

Wrona, Olga; Rafińska, Katarzyna; Krakowska-Sieprawska, Aneta; Buszewski, Bogusław

doi:10.3390/molecules26102994

Cited by 5 publications

(5 citation statements)

References 30 publications

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“…The highest MYR, as the most abundant flavonoid in bound extract, had foreign cultivar Florida 66 (502.2 μg/g dm), which is 4.3 times more than the lowest value in OS 101 (117.1 μg/g dm) ( Figure 3 b; Table S4 ). In alfalfa samples, in addition to the flavonoids found in our research, some authors also mentioned glabridin, naringin, and luteolin as major flavonoids [ 13 , 36 , 40 , 41 , 42 ].…”

Section: Resultssupporting

confidence: 65%

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Characterization of Forage Quality, Phenolic Profiles, and Antioxidant Activity in Alfalfa (Medicago sativa L.)

Horvat

Vuletić

Andrić

et al. 2022

Plants

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Alfalfa (Medicago sativa L.) is one of the most important forage species and is recently more in focus for human consumption mainly due to its content of bioactive phenolics. Samples of the seventeen alfalfa cultivars/populations were collected at the Agricultural Institute Osijek with the aim to evaluate their forage quality, phenolic profiles, and antioxidant potential. Significant differences (p < 0.05) existed among studied alfalfa in all analyzed traits. The cultivar OS 99 and populations L7 and L20 were characterized by high crude protein content (22.5–24.7%) and the lowest neutral (40.2–42.9%) and acid detergent fibres (33–35.5%). The soluble-free phenolics from alfalfa leaves were extracted by methanol while insoluble cell-wall bound phenolics were released by alkaline hydrolysis. The bound phenolic extract showed a stronger DPPH scavenging capacity (20.8 mg TE/g dm) than the soluble (11.4 mg TE/g dm). The HPLC data revealed that more phenolics were found in the bound (3638.0 μg/g dm) than in the soluble form (912.3 μg/g dm). In the soluble extract of the alfalfa leaves, the major compound was catechin (338.3 μg/g dm), while rutin, epicatechin, and ferulic acid were minor ones. In the bound phenolic extract, the most abundant was ferulic (2198.2 μg/g dm) and p-coumaric acid (983.7 μg/g dm), followed by myricetin, apigenin, and quercetin. The principal component analysis revealed that alfalfa cultivars/populations were better discriminated based on the data on phenolics, rather than on forage quality. The cultivars/populations Florida 66, OS 66, L 40, L 42, Seed Force 4, and Torlesse were the most interesting in terms of phenolic health-promoting characteristics.

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Section: Resultssupporting

confidence: 65%

“…), used analytical technique (HPLC, HPLC-MS, HPLC-MS/MS, etc. ), and alfalfa part (aerial or roots) [ 16 , 35 , 36 ]. Newby et al [ 37 ] determined the dominance of alkali-labile bound phenolic acids in relation to soluble ones (less than 2%).…”

Section: Resultsmentioning

confidence: 99%

Characterization of Forage Quality, Phenolic Profiles, and Antioxidant Activity in Alfalfa (Medicago sativa L.)

Horvat

Vuletić

Andrić

et al. 2022

Plants

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“…The high-pressure treatment in the SFE process could better destroy the camellia seed cells, rendering the extractant to enter the cells to extract the oil. Moreover, the high oil yield of SFE was also connected with the excellent extraction performance of CO2 (Wrona et al, 2021). Figure 3B shows the microstructure of camellia seed cake after AE.…”

Section: Microstructure Analysis Of Camellia Seed Cakes With Differen...mentioning

confidence: 86%

Quality comparison of camellia (Camellia oleifera C.Abel) seed oil with different extraction methods

He¹,

Ding²,

Lin³

et al. 2023

IFRJ

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The quality of camellia seed oil (CSO) varies with the oil extraction methods. In the present work, the oil yield, physicochemical properties, bioactive compounds, fatty acid composition, and Fourier transform infrared spectra of CSOs prepared by supercritical fluid, aqueous, pressing, and solvent extraction were explored systematically. Additionally, the microstructure of camellia seed cake after oil extraction was observed by scanning electron microscopy. Results showed that supercritical fluid extraction had the highest oil yield (92.42%), and the extracted oil was also superior to the other methods in the contents of polyphenol, β-sitosterol, and squalene, which were 89.34, 3173.23, and 6.20 mg/kg, respectively. Moreover, CSO extracted by supercritical fluid extraction had lower peroxide value and better colour indexes. In terms of fatty acid composition, CSOs extracted by supercritical fluid, pressing, and solvent extraction were similar, while CSO extracted by aqueous extraction had higher saturated fatty acid contents and lower unsaturated fatty acid contents than the other samples. Fourier transform infrared spectra analysis showed that the extraction methods had no significant effect on the chemical functional groups of CSOs. Scanning electron microscopy revealed that supercritical fluid extraction and solvent extraction could more effectively promote the release of oil from camellia seeds. In general, the quality of CSOs extracted by different methods had significant differences, and supercritical fluid extraction could be a promising extraction method for CSO.

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“…However, it is difficult to implement this technology at a large commercial scale due to the large amounts of co-solvents required. Consequently, it was recently proposed that CO 2 should be used as the only solvent to extract polyphenols from produce using SFAE [ 53 ]. These authors optimized the temperature, pressure, and solvent flow required to isolate phytochemicals from alfalfa and showed that SFAE could be used to efficiently isolate both phenolic acids and flavonoids.…”

Section: Isolation Of Bioactive Agents From Botanical Sourcesmentioning

confidence: 99%

Encapsulation of Bioactive Phytochemicals in Plant-Based Matrices and Application as Additives in Meat and Meat Products

Domínguez¹,

Pateiro²,

Munekata³

et al. 2021

Molecules

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The development of plant-based functional food ingredients has become a major focus of the modern food industry as a response to changes in consumer attitudes. In particular, many consumers are switching to a plant-based diet because of their concerns about animal-derived foods on the environment, human health, and animal welfare. There has therefore been great interest in identifying, isolating, and characterizing functional ingredients from botanical sources, especially waste streams from food and agricultural production. However, many of these functional ingredients cannot simply be incorporated into foods because of their poor solubility, stability, or activity characteristics. In this article, we begin by reviewing conventional and emerging methods of extracting plant-based bioactive agents from natural resources including ultrasound-, microwave-, pulsed electric field- and supercritical fluid-based methods. We then provide a brief overview of different methods to characterize these plant-derived ingredients, including conventional, chromatographic, spectroscopic, and mass spectrometry methods. Finally, we discuss the design of plant-based delivery systems to encapsulate, protect, and deliver these functional ingredients, including micelles, liposomes, emulsions, solid lipid nanoparticles, and microgels. The potential benefits of these plant-based delivery systems are highlighted by discussing their use for incorporating functional ingredients into traditional meat products. However, the same technologies could also be employed to introduce functional ingredients into plant-based meat analogs.

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Comparative Studies of Selected Criteria Enabling Optimization of the Extraction of Polar Biologically Active Compounds from Alfalfa with Supercritical Carbon Dioxide

Cited by 5 publications

References 30 publications

Characterization of Forage Quality, Phenolic Profiles, and Antioxidant Activity in Alfalfa (Medicago sativa L.)

Characterization of Forage Quality, Phenolic Profiles, and Antioxidant Activity in Alfalfa (Medicago sativa L.)

Quality comparison of camellia (Camellia oleifera C.Abel) seed oil with different extraction methods

Encapsulation of Bioactive Phytochemicals in Plant-Based Matrices and Application as Additives in Meat and Meat Products

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