Comparative studies of thermotolerance: different modes of heat acclimation between tolerant and intolerant aquatic plants of the genus Potamogeton

Amano, Momoe; Iida, Satoko; Kosuge, Keiko

doi:10.1093/aob/mcr300

Cited by 33 publications

(38 citation statements)

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“…Increasing evidence shows that alternative splicing (AS) is a critical posttranscriptional event and plays an important role in plant stress responses (Mazzucotelli et al, 2008;Mastrangelo et al, 2012). However, the AS of Hsfs in plants is largely unknown, although AS events have been experimentally identified in a few plant genes, including alfalfa (Medicago sativa) Hsf1 (He et al, 2007), Arabidopsis HsfA2 (Sugio et al, 2009), and genus Potamogeton HsfA2a2 (Amano et al, 2012). These Hsf splice variants contain premature termination codons and are degraded through the nonsense-mediated mRNA decay (NMD; He et al, 2007;Sugio et al, 2009;Amano et al, 2012).…”

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confidence: 99%

“…However, the AS of Hsfs in plants is largely unknown, although AS events have been experimentally identified in a few plant genes, including alfalfa (Medicago sativa) Hsf1 (He et al, 2007), Arabidopsis HsfA2 (Sugio et al, 2009), and genus Potamogeton HsfA2a2 (Amano et al, 2012). These Hsf splice variants contain premature termination codons and are degraded through the nonsense-mediated mRNA decay (NMD; He et al, 2007;Sugio et al, 2009;Amano et al, 2012). Thus, AS-NMD may be involved in regulating the level of full-length mRNAs of these three plant Hsfs.…”

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confidence: 99%

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An Autoregulatory Loop Controlling Arabidopsis HsfA2 Expression: Role of Heat Shock-Induced Alternative Splicing

et al. 2013

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Heat shock transcription factorA2 (HsfA2) is a key regulator in response to heat stress in Arabidopsis (Arabidopsis thaliana), and its heat shock (HS)-induced transcription regulation has been extensively studied. Recently, alternative splicing, a critical posttranscriptional event, has been shown to regulate HS-inducible expression of HsfA2; however, the molecular mechanism remains largely unknown. Here, we demonstrate a new heat stress-induced splice variant, HsfA2-III, is involved in the self-regulation of HsfA2 transcription in Arabidopsis. HsfA2-III is generated through a cryptic 59 splice site in the intron, which is activated by severe heat (42°C-45°C). We confirmed that HsfA2-III encodes a small truncated HsfA2 isoform (S-HsfA2) by an immunoblot assay with anti-S-HsfA2 antiserum. S-HsfA2 has an extra leucine-rich motif next to its carboxyl-terminal truncated DNA-binding domain. The biological significance of S-HsfA2 was further demonstrated by its nuclear localization and heat shock element (HSE)-binding ability. In yeast (Saccharomyces cerevisiae), the leucine-rich motif can inhibit the transcriptional activation activity of S-HsfA2, while it appears not to be required for the truncated DNA-binding domain-mediated binding ability of S-HsfA2-HSE. Further results reveal that S-HsfA2 could bind to the TATA box-proximal clusters of HSE in the HsfA2 promoter to activate its own transcription. This S-HsfA2-modulated HsfA2 transcription is not mediated through homodimer or heterodimer formation with HsfA1d or HsfA1e, which are known transcriptional activators of HsfA2. Altogether, our findings provide new insights into how HS posttranscriptionally regulates HsfA2 expression. Severe HS-induced alternative splicing also occurs in four other HS-inducible Arabidopsis Hsf genes, suggesting that it is a common feature among Arabidopsis Hsfs.

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An Autoregulatory Loop Controlling Arabidopsis HsfA2 Expression: Role of Heat Shock-Induced Alternative Splicing

et al. 2013

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“…Previous studies on animals (Sorensen and Loeschcke 2001;Buckley et al 2001;Tomanek 2010) and plants (Knight and Ackerly 2003;Barua et al 2008;Knight 2010;Amano et al 2012) have shown variability in the threshold temperature needed to induce the HS response, and differences exist among species in the levels of HSPs expressed during stress. In a study of the plant Encelia (E. formosa from the Mojave desert and E. californica from the coast), it was reported that the desert species, E. formosa, produced more sHSPs above 45°C than the coastal species (E. californica) did.…”

Section: Relationship Of Thermotolerance Patterns To Species Habitatmentioning

confidence: 97%

“…Studies of the chloroplast sHSPs (Knight andAckerly 2001, 2003;Barua et al 2003;Wang and Luthe 2003;Knight 2010) have found varying levels of HSP gene expression and differences in organismal thermotolerance. More recently Amano et al (2012) reported differences in HSF and HSP (specifically CP-sHSP) gene expression and significant differences in thermotolerance in two Potamogeton species. Other studies in plants have shown the some HSP genes have undergone positive selection (Wu et al 2007) and that differences in expression levels can have fitness consequences (Tonsor et al 2008).…”

Section: Introductionmentioning

confidence: 98%

Patterns of thermotolerance, chlorophyll fluorescence, and heat shock gene expression vary among fourBoecheraspecies andArabidopsis thaliana

Halter

Simonetti

Suguitan

et al. 2017

Botany

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Thermotolerance is a property of all organisms, but owing to their sessile nature, this trait is particularly important in plants. Basal thermotolerance is based on inherent tolerance to heat stress. Acquired thermotolerance is attained through stress-induced gene expression, often of those genes encoding heat shock proteins (HSPs). Both basal and acquired thermotolerance have been extensively studied in model species such as Arabidopsis thaliana (L.) Heynh., but much less is known about thermotolerance in wild plant species. The aims of this study were to examine the basal and acquired thermotolerance of four species of Boechera, and of A. thaliana. Mots-clés : Arabidopsis, Boechera, fluorescence de la chlorophylle, expression génique induite par la chaleur, stress thermique, thermotolérance.

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“…Warming may decrease photosynthesis and increase respiration (Ryan 1991), thereby impacting the distribution, modes of reproduction, germination, growth, and dormancy of freshwater SAV (Welch 1952;Barko and Smart 1981;Lacoul and Freedman 2006). However, the response of freshwater aquatic plants to climate warming is species-specific, and varies even for locally adapted "biotypes" (e.g., Barko and Smart 1981;Pip 1989;Svensson and Wigren-Svensson 1992;Santamaría and Van Vierssen 1997;Rooney and Kalff 2000;Sala et al 2000;Amano, Iida, and Kosuge 2012). Some species exhibit earlier germination and increased productivity, while others do not (Mckee et al 2002;Lacoul and Freedman 2006).…”

Section: A Warming Estuarymentioning

confidence: 99%

Twenty-first century climate change and submerged aquatic vegetation in a temperate estuary: the case of Chesapeake Bay

Arnold

Zimmerman

Engelhardt

et al. 2017

Ecosyst Health Sustain

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Introduction: The Chesapeake Bay was once renowned for expansive meadows of submerged aquatic vegetation (SAV). However, only 10% of the original meadows survive. Future restoration efforts will be complicated by accelerating climate change, including physiological stressors such as a predicted mean temperature increase of 2-6°C and a 50-160% increase in CO 2 concentrations. Outcomes: As the Chesapeake Bay begins to exhibit characteristics of a subtropical estuary, summer heat waves will become more frequent and severe. Warming alone would eventually eliminate eelgrass (Zostera marina) from the region. It will favor native heat-tolerant species such as widgeon grass (Ruppia maritima) while facilitating colonization by non-native seagrasses (e.g., Halodule spp.). Intensifying human activity will also fuel coastal zone acidification and the resulting high CO 2 /low pH conditions may benefit SAV via a "CO 2 fertilization effect." Discussion: Acidification is known to offset the effects of thermal stress and may have similar effects in estuaries, assuming water clarity is sufficient to support CO 2 -stimulated photosynthesis and plants are not overgrown by epiphytes. However, coastal zone acidification is variable, driven mostly by local biological processes that may or may not always counterbalance the effects of regional warming. This precarious equipoise between two forcesthermal stress and acidification -will be critically important because it may ultimately determine the fate of cool-water plants such as Zostera marina in the Chesapeake Bay. Conclusion: The combined impacts of warming, coastal zone acidification, water clarity, and overgrowth of competing algae will determine the fate of SAV communities in rapidly changing temperate estuaries.

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Comparative studies of thermotolerance: different modes of heat acclimation between tolerant and intolerant aquatic plants of the genus Potamogeton

Cited by 33 publications

References 59 publications

An Autoregulatory Loop Controlling Arabidopsis HsfA2 Expression: Role of Heat Shock-Induced Alternative Splicing

An Autoregulatory Loop Controlling Arabidopsis HsfA2 Expression: Role of Heat Shock-Induced Alternative Splicing

Patterns of thermotolerance, chlorophyll fluorescence, and heat shock gene expression vary among fourBoecheraspecies andArabidopsis thaliana

Twenty-first century climate change and submerged aquatic vegetation in a temperate estuary: the case of Chesapeake Bay

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