Comparative Studies of Transglutaminase Activity and Substrates in Different Organs of Helianthus tuberosus

Falcone, P.; Serafini-Fracassini, Donatella; Duca, Stefano Del

doi:10.1016/s0176-1617(11)80421-9

Cited by 40 publications

(34 citation statements)

References 25 publications

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“…Both the large subunit of Rubisco (Margosiak et al, 1990) and chlorophyll a/b binding proteins (Del Duca et al, 1994) have been identified as major TGase substrates. A potential role for TGase in cross-linking plant extracellular proteins has been suggested in two studies (Falcone et al, 1993;Serafini-Fracassini et al, 1995). The present study demonstrates that C. reinhardtii secretes an extracellular TGase immunologically related to guinea pig tissue TGase whose substrates include cell wall HRGPs, and that inhibition of this activity disrupts normal wall assembly.…”

supporting

confidence: 55%

A Transglutaminase Immunologically Related to Tissue Transglutaminase Catalyzes Cross-Linking of Cell Wall Proteins inChlamydomonas reinhardtii

Waffenschmidt¹,

Kusch

Woessner

1999

Plant Physiology

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The addition of primary amines to the growth medium of the unicellular green alga Chlamydomonas reinhardtii disrupts cell wall assembly in both vegetative and zygotic cells. Primary amines are competitive inhibitors of the protein-cross-linking activity of transglutaminases. Two independent assays for transglutaminase confirmed a burst of extracellular activity during the early stages of cell wall formation in both vegetative cells and zygotes. When noninhibiting levels of a radioactive primary amine ( 14 C-putrescine) were added to the growth medium, both cell types were labeled in a reaction catalyzed by extracellular transglutaminase. The radioactive label was found specifically in the cell wall proteins of both cell types, and acid hydrolysis of the labeled material released unmodified 14 C-putrescine. Western blots of the proteins secreted at the times of maximal transglutaminase activity in both cell types revealed a single highly cross-reactive 72-kD band when screened with antibodies to guinea pig tissue transglutaminase. Furthermore, the proteins immunoprecipitated by this antiserum in vivo exhibited transglutaminase activity. We propose that this transglutaminase is responsible for an early cell wall protein cross-linking event that temporally precedes the oxidative cross-linking mediated by extracellular peroxidases.

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supporting

confidence: 55%

A Transglutaminase Immunologically Related to Tissue Transglutaminase Catalyzes Cross-Linking of Cell Wall Proteins inChlamydomonas reinhardtii

Waffenschmidt¹,

Kusch

Woessner

1999

Plant Physiology

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“…cytoskeleton, cell walls, nuclei, chloroplasts and vacuoles. Subcellular compartmentation of plant TGases has been observed in Helianthus leaves and maize calli; 27,50,51 in some compartments TGase could play a specific role. As suggested by the present data, the highest production of bis-derivatives, especially bis-PU, occurs in the phases of development and anthesis (stages 3 ± 5) when the corolla changes shape in order to favour the pollination process.…”

Section: Corolla Of Undetached¯owersmentioning

confidence: 99%

Transglutaminase activity during senescence and programmed cell death in the corolla of tobacco (Nicotiana tabacum) flowers

et al. 2002

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Corolla life span of undetached flowers of Nicotiana tabacum was divided into stages from the closed corolla (stage 1) through anthesis (stage 5) to death (stage 9). Senescence began around stage 6 in the proximal part, concomitantly with DNA laddering. Nuclear blebbing, DNA laddering, cell wall modification, decline in protein, water, pigment content and membrane integrity were observed during senescence and PCD. Transglutaminase activity was measured as mono-and bis-derivatives of putrescine (mono-PU; bis-PU) and bis-derivatives of spermidine (bis-SD). Bis-derivatives decreased with the progression of senescence, while mono-PU increased during early senescence; derivatives were present in different amounts in the proximal and distal parts of the corolla. In excised flowers, exogenous spermine delayed senescence and PCD, and caused an increase in free and acid-soluble conjugated PA levels. Bis-PU was the most abundant PA-derivative before DNA laddering stage; thereafter, bis-PU generally decreased and mono-PU became the most abundant derivative.

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“…TGases have been found in animal tissues and body fluids, fish, plants, and microorganisms (Ando et al, 1989;Falcone, Serafini-Fracassini, & del Duca, 1993;Folk, 1980;Lilley, Skill, Griffin, & Bonner, 1998;Yasueda et al, 1995). In food processing, this enzyme has been used to promote polymerization of proteins, texture improvement, meat restructuring, gel formation, and to increase nutritional value of proteins as well (Alves-Macedo, Duraes, & Hurami, 2007;Griffin, Casadio, & Bergamini, 2002;Motoki & Seguro, 1998).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of sorghum grain hydrolysates and dried distillers grains with solubles for the production of microbial transglutaminase

Rodríguez-Castillejos

Luis

Vázquez

et al. 2013

CyTA - Journal of Food

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Sorghum grains can be used as a source of fermentable sugars to reduce the culture media cost for microbial growth of Streptomyces and the production of microbial transglutaminase (MTGase). In the production of ethanol from corn, dried distillers grains with solubles (DDGSs) are generated as waste. The aim of this work is to elucidate the biotechnological production of transglutaminase by Streptomyces mobaraensis NRRL-3191 grown in a medium containing sorghum grains hydrolysates and DDGS is feasible. The results showed that casein has a great effect on the production of MTGase while DDGS did not show any significant effect on the range of study. The model obtained predicts 0.66 U/mL of MTGase activity using enzymatic hydrolysates of sorghum grains supplemented with yeast extract (2 g/L), DDGS (2 g/L), and casein (19.5 g/L).Keywords: transglutaminase; Streptomyces mobaraensis; sorghum; dried distillers grains with solubles; casein; hydrolysates Los granos de sorgo se pueden utilizar como fuente de azúcares fermentables para reducir los costes de medios de cultivo para el crecimiento microbiano de Streptomyces y la producción de transglutaminasa microbiana (MTGasa). En la producción de etanol a partir de maíz, se generan como residuos granos de destilería secos con solubles (DDGS). El objetivo de este trabajo fue determinar si la producción biotecnológica de transglutaminasa por Streptomyces mobaraensis NRRL-3191 cultivados en un medio que contiene hidrolizados de granos de sorgo y DDGS es factible. Los resultados mostraron que la caseína tiene un gran efecto en la producción de MTGasa mientras que DDGS no mostraron un efecto significativo en el intervalo de estudio. El modelo obtenido predice 0.66 U/mL de actividad MTGase usando hidrolizados enzimáticos de granos de sorgo suplementados con extracto de levadura (2 g/L), DDGS (2 g/L) y caseína (19,5 g/L).

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Comparative Studies of Transglutaminase Activity and Substrates in Different Organs of Helianthus tuberosus

Cited by 40 publications

References 25 publications

A Transglutaminase Immunologically Related to Tissue Transglutaminase Catalyzes Cross-Linking of Cell Wall Proteins inChlamydomonas reinhardtii

A Transglutaminase Immunologically Related to Tissue Transglutaminase Catalyzes Cross-Linking of Cell Wall Proteins inChlamydomonas reinhardtii

Transglutaminase activity during senescence and programmed cell death in the corolla of tobacco (Nicotiana tabacum) flowers

Evaluation of sorghum grain hydrolysates and dried distillers grains with solubles for the production of microbial transglutaminase

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